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1.发明公开METHODS OF PREPARING FEEDER CELLS-FREE, XENO-FREE HUMAN EMBRYONIC STEM CELLS AND STEM CELL CULTURES PREPARED USING SAME 有权用于生产无滋养层细胞，XENOFREIER胚胎干细胞和人类所产生干细胞培养
公开(公告)号：EP1572984A4
公开(公告)日：2007-02-28
申请号：EP03813286
申请日：2003-12-07
IPC分类号： C12N5/02 , C12N5/0735 , C12N5/00
CPC分类号： C12N5/0606 , C12N5/0031 , C12N5/0037 , C12N5/0043 , C12N5/0056 , C12N5/0607 , C12N5/0611 , C12N5/0623 , C12N5/0647 , C12N5/0696 , C12N2500/25 , C12N2500/84 , C12N2500/90 , C12N2500/98 , C12N2501/10 , C12N2501/115 , C12N2501/119 , C12N2501/125 , C12N2501/15 , C12N2501/155 , C12N2501/235 , C12N2501/33 , C12N2501/998 , C12N2533/52
摘要： The present invention is of methods of establishing and p ropagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells.
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公开(公告)号：EP1554373A4
公开(公告)日：2006-07-12
申请号：EP03751238
申请日：2003-10-07
IPC分类号： C12N5/0735 , C12N5/074 , C12N5/00 , C12N15/00
CPC分类号： C12N5/0606 , C12N5/0031 , C12N5/0043 , C12N5/0056 , C12N5/0603 , C12N5/0611 , C12N5/0665 , C12N5/0695 , C12N5/0696 , C12N2500/00 , C12N2500/99 , C12N2502/13 , C12N2502/1323 , C12N2506/02 , C12N2533/50 , C12N2533/90
摘要： A cell culture comprising human foreskin cells, the human foreskin cells being capable of maintaining stem cells in an undifferentiated state when co-cultured therewith.
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3.发明公开
公开(公告)号：EP2646543A4
公开(公告)日：2014-09-03
申请号：EP11844229
申请日：2011-12-01
CPC分类号： A61K35/30 , C12N5/0062 , C12N5/0621 , C12N2501/11 , C12N2501/155 , C12N2501/33 , C12N2502/085 , C12N2502/1323 , C12N2506/02 , C12N2506/45 , C12N2533/52 , C12N2533/54 , C12N2533/90 , G01N33/5058 , G01N33/53
摘要： A method of generating a population of corneal epithelial cells is disclosed. The method comprises culturing human pluripotent stem cells in corneal fibroblast-conditioned medium on a solid surface comprising an extracellular matrix component thereby generating the population of corneal epithelial cells. Isolated cell populations and corneal tissues are also disclosed, as well as uses thereof.
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公开(公告)号：EP1567639A4
公开(公告)日：2005-12-21
申请号：EP03812259
申请日：2003-12-03
CPC分类号： C12N5/0677 , C12N2500/38 , C12N2500/90 , C12N2501/58 , C12N2506/02
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5.发明公开
公开(公告)号：EP2139989A4
公开(公告)日：2010-07-07
申请号：EP08720058
申请日：2008-03-27
CPC分类号： C12N5/0603 , C12N2501/155 , C12N2501/40 , C12N2501/60 , C12N2502/1394 , C12N2506/02 , C12N2510/00
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6.发明公开CULTURE MEDIA, CELL CULTURES AND METHODS OF CULTURING PLURIPOTENT STEM CELLS IN AN UNDIFFERENTIATED STATE 有权科学家，ZELLKULTUREN UND VERFAHREN ZUR KULTIVIERUNG PLURIPOTENTER STAMMZELLEN IN UNDIFFERENZIERTEM ZUSTAND
公开(公告)号：EP2499236A4
公开(公告)日：2013-05-22
申请号：EP10829622
申请日：2010-11-11
IPC分类号： C12N5/02 , C12N5/071 , C12N5/0735
CPC分类号： C12N5/0602 , C12N5/0606 , C12N5/0696 , C12N2500/36 , C12N2500/90 , C12N2500/92 , C12N2500/98 , C12N2500/99 , C12N2501/115 , C12N2501/15 , C12N2501/23 , C12N2506/45
摘要： Provided are serum-free culture media which comprise basic fibroblast growth factor (bFGF), transforming growth factor beta-3, ascorbic acid, xeno-free serum replacement and a lipid mixture. The media may also comprise an IL6R/IL6 chimera, or leukemia inhibitory factor (LIF); wherein the culture medium is capable of maintaining pluripotent stem cells in an undifferentiated state in the absence of feeder cell support. Also provided are cell cultures comprising pluripotent stem cells such as human embryonic stem cells and induced pluripotent stem (iPS) cells, and methods of using the same for expanding pluripotent stem cells in an undifferentiated state using two-dimensional or three-dimensional culture systems. Methods of expanding iPS cells in a suspension culture devoid of substrate adherence and cell encapsulation are also provided.
摘要翻译： 提供了包含碱性成纤维细胞生长因子（bFGF），转化生长因子β-3，抗坏血酸，不含异种血清的血清替代物和脂质混合物的无血清培养基。 培养基还可以包含IL6R / IL6嵌合体或白血病抑制因子（LIF）; 其中所述培养基能够在不存在饲养细胞支持的情况下将多能干细胞保持在未分化状态。 还提供了包含多能干细胞如人胚胎干细胞和诱导的多能干细胞（iPS）细胞的细胞培养物，以及使用该多能干细胞用于使用二维或三维培养系统在未分化状态下扩增多能干细胞的方法。 还提供了在没有底物粘附和细胞包封的悬浮培养物中扩增iPS细胞的方法。
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7.发明公开
公开(公告)号：EP1981971A4
公开(公告)日：2009-08-12
申请号：EP07700739
申请日：2007-01-11
IPC分类号： C12N5/077
CPC分类号： C12N5/0662 , A61K35/12 , C12N5/0652 , C12N2500/38 , C12N2501/39 , C12N2506/02 , C12N2533/40
摘要： Methods of generating and expanding proliferative, multipotent connective tissue progenitor cells from adult stem cells are provided. Also provided are methods of generating functional tendon grafts in vitro and bone, cartilage and connective tissues in vivo using the isolated cell preparation of connective tissue progenitor cells.
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8.发明公开METHODS OF GENERATING STEM CELLS AND EMBRYONIC BODIES CARRYING DISEASE-CAUSING MUTATIONS AND METHODS OF USING SAME FOR STUDYING GENETIC DISORDERS 审中-公开程序于产生干细胞和胚胎机构，致病突变的磨损和方法及其使用中的遗传性疾病的研究
公开(公告)号：EP1694825A4
公开(公告)日：2008-06-18
申请号：EP04799354
申请日：2004-11-15
IPC分类号： C12N5/00 , A61K20060101 , C12N5/02 , C12N5/0735 , C12N5/10 , C12N15/63 , C12Q1/68
CPC分类号： C12N5/0606 , C12N2510/00 , C12Q1/6883 , C12Q2600/156 , C12Q2600/158
摘要： Stem cells, stem cell lines and differentiated cells, tissues and organs which carry disease-causing mutations are provided. There is also provided a method of identifying agents suitable for treating disorders associated with at least one disease-causing mutations such as myotonic dystrophy and van Waardenburg syndrome.
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9.发明公开HUMAN EMBRYONIC STEM CELL-DERIVED CONNECTIVE TISSUE PROGENITORS FOR TISSUE ENGINEERING 审中-公开作者人胚胎干细胞衍生的结缔组织预组织重建
公开(公告)号：EP1981970A4
公开(公告)日：2009-10-21
申请号：EP07700738
申请日：2007-01-11
IPC分类号： C12N5/077
CPC分类号： C12N5/0662 , A61K35/12 , C12N5/0652 , C12N2500/38 , C12N2501/39 , C12N2506/02 , C12N2533/40
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公开(公告)号：EP1490476A4
公开(公告)日：2007-01-17
申请号：EP03745639
申请日：2003-03-26
CPC分类号： C12N5/069 , C12N2506/02
摘要： The invention is a population of embryonic endothelial cells produced in vitro from human embryonic stem cells. The cells produce platelet endothelial cell adhesion molecule-1 and are vasculogenic. The cells may be combined with a cell support substrate, seeded on a polymer matrix, or combined with a cell-support substrate that is infused into a polymer matrix. The cells may also be injected directly into a tissue site.
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