公开(公告)号：EP2840140A1

公开(公告)日：2015-02-25

申请号：EP14188820.6

申请日：2013-12-12

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College ,  The Rockefeller University

发明人： Zhang, Feng ,  Bikard, David Olivier ,  Cox, David Benjamin Turitz ,  Jiang, Wenyan ,  Marraffini, Luciano

IPC分类号： C12N15/63

CPC分类号： C12N15/113 ,  C12N9/22 ,  C12N15/102 ,  C12N15/1082 ,  C12N15/63 ,  C12N15/79 ,  C12N15/907 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2320/11 ,  C12N2320/30 ,  C12N2750/14143 ,  G06F19/18 ,  G06F19/22

摘要： The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.

摘要翻译： 本发明提供了用于操纵靶序列的序列和/或活性的系统，方法和组合物。 提供了载体和载体系统，其中一些编码CRISPR复合物的一个或多个组分，以及用于设计和使用这些载体的方法。 还提供了在真核细胞中引导CRISPR复合物形成的方法以及通过引入使用CRISPR / Cas系统的精确突变来选择特异性细胞的方法。

公开(公告)号：EP2848690B1

公开(公告)日：2020-08-19

申请号：EP14190376.5

申请日：2013-12-12

申请人： The Broad Institute, Inc. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  President and Fellows of Harvard College ,  The Rockefeller University

发明人： Zhang, Feng ,  Bikard, David Olivier ,  Cox, David Benjamin Turitz ,  Jiang, Wenyan ,  Marraffini, Luciano ,  Habib, Naomi ,  Cong, Le

IPC分类号： C12N15/63 ,  C12N9/22 ,  C12N15/70 ,  C12N15/74 ,  C12N15/85 ,  C12N15/10

公开(公告)号：EP2840140B1

公开(公告)日：2016-11-16

申请号：EP14188820.6

申请日：2013-12-12

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College ,  The Rockefeller University

发明人： Zhang, Feng ,  Bikard, David Olivier ,  Cox, David Benjamin Turitz ,  Jiang, Wenyan ,  Marraffini, Luciano

IPC分类号： C12N15/63

CPC分类号： C12N15/113 ,  C12N9/22 ,  C12N15/102 ,  C12N15/1082 ,  C12N15/63 ,  C12N15/79 ,  C12N15/907 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2320/11 ,  C12N2320/30 ,  C12N2750/14143 ,  G06F19/18 ,  G06F19/22

公开(公告)号：EP2848690A1

公开(公告)日：2015-03-18

申请号：EP14190376.5

申请日：2013-12-12

申请人： The Broad Institute, Inc. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  President and Fellows of Harvard College ,  The Rockefeller University

发明人： Zhang, Feng ,  Bikard, David Olivier ,  Cox, David Benjamin Turitz ,  Jiang, Wenyan ,  Marraffini, Luciano ,  Habib, Naomi ,  Cong, Le

IPC分类号： C12N15/63 ,  C12N9/22 ,  C12N15/70 ,  C12N15/74 ,  C12N15/85 ,  C12N15/10

摘要： The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.

摘要翻译： 本发明提供了用于操纵靶序列的序列和/或活性的系统，方法和组合物。 提供了载体和载体系统，其中一些编码CRISPR复合物的一个或多个组分，以及设计和使用这些载体的方法。 还提供了指导真核细胞中CRISPR复合物形成的方法和通过利用CRISPR / Cas系统引入精确突变来选择特定细胞的方法。

公开(公告)号：EP2821413B2

公开(公告)日：2019-10-02

申请号：EP14179189.7

申请日：2012-11-30

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College

发明人： Zhang, Feng ,  Cong, Le

IPC分类号： C07K14/03 ,  C12Q1/68 ,  C07K14/195 ,  C07K14/47 ,  C12N5/07 ,  C12N5/071 ,  C12N9/12 ,  C12N9/22 ,  C12N15/63

公开(公告)号：EP3327127A1

公开(公告)日：2018-05-30

申请号：EP17199166.4

申请日：2013-12-12

申请人： The Broad Institute, Inc. ,  Massachusetts Institute Of Technology ,  President and Fellows of Harvard College

发明人： Zhang, Feng ,  Heidenreich, Matthias ,  Ran, Fei ,  Swiech, Lukasz

IPC分类号： C12N15/63 ,  C12N15/10

CPC分类号： C12N15/907 ,  A01K67/0275 ,  A01K67/0278 ,  A01K2217/05 ,  A01K2217/052 ,  A01K2217/07 ,  A01K2217/072 ,  A01K2227/105 ,  A01K2267/03 ,  C12N9/22 ,  C12N9/96 ,  C12N15/01 ,  C12N15/102 ,  C12N15/63 ,  C12N15/8213 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/86 ,  C12N15/90 ,  C12N2800/22

摘要： The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are delivery systems and tissues or organ which are targeted as sites for delivery. Also provided are vectors and vector systems some of which encode one or more components of a CRISPR complex, as well as methods for the design, and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity and to edit or modify a, target site in a genomic locus of interest to alter or improve the status of a disease or a condition.

公开(公告)号：EP2821413B1

公开(公告)日：2016-09-07

申请号：EP14179189.7

申请日：2012-11-30

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College

发明人： Zhang, Feng ,  Cong, Le

IPC分类号： C07K14/03 ,  C12Q1/68 ,  C07K14/195 ,  C07K14/47 ,  C12N5/07 ,  C12N5/071 ,  C12N9/12 ,  C12N9/22 ,  C12N15/63

摘要： The invention relates to methods of altering expression of a genomic locus of interest or specifically targeting a genomic locus of interest in an animal cell, which may involve contacting the genomic locus with a non-naturally occurring or engineered composition that includes a deoxyribonucleic acid (DNA) binding polypeptide having a N-terminal capping region, a DNA binding domain comprising at least five or more Transcription activator-like effector (TALE) monomers and at least one or more half-monomers specifically ordered to target the genomic locus of interest, and a C-terminal capping region, wherein the polypeptide includes at least one or more effector domains, and wherein the polypeptide is encoded by and translated from a codon optimized nucleic acid molecule so that the polypeptide preferentially binds to the DNA of the genomic locus.

公开(公告)号：EP2821413A1

公开(公告)日：2015-01-07

申请号：EP14179189.7

申请日：2012-11-30

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College

发明人： Zhang, Feng ,  Cong, Le

IPC分类号： C07K14/03 ,  C12Q1/68 ,  C07K14/195 ,  C07K14/47 ,  C12N5/07 ,  C12N5/071 ,  C12N9/12 ,  C12N9/22 ,  C12N15/63

摘要： The invention relates to methods of altering expression of a genomic locus of interest or specifically targeting a genomic locus of interest in an animal cell, which may involve contacting the genomic locus with a non-naturally occurring or engineered composition that includes a deoxyribonucleic acid (DNA) binding polypeptide having a N-terminal capping region, a DNA binding domain comprising at least five or more Transcription activator-like effector (TALE) monomers and at least one or more half-monomers specifically ordered to target the genomic locus of interest, and a C-terminal capping region, wherein the polypeptide includes at least one or more effector domains, and wherein the polypeptide is encoded by and translated from a codon optimized nucleic acid molecule so that the polypeptide preferentially binds to the DNA of the genomic locus.

摘要翻译： 本发明涉及改变感兴趣的基因组座位的表达或特异性靶向动物细胞中的目的基因座的方法，其可能涉及使基因组座位与包含脱氧核糖核酸（DNA）的非天然存在或工程化组合物接触 ）结合多肽，DNA结合结构域包含至少五个或更多个转录激活子样效应子（TALE）单体和至少一种或多种特异性命令靶向感兴趣的基因组座位的半单体，以及 C端封端区，其中所述多肽包括至少一个或多个效应结构域，并且其中所述多肽由密码子优化的核酸分子编码和翻译，使得所述多肽优先结合所述基因组基因座的DNA。

公开(公告)号：EP3470519B1

公开(公告)日：2020-02-12

申请号：EP18186003.2

申请日：2016-06-17

申请人： The Broad Institute, Inc. ,  Massachusetts Institute Of Technology ,  President and Fellows of Harvard College

发明人： Zhang, Feng ,  Zetsche, Bernd ,  Gootenberg, Jonathan, S. ,  Abudayyeh, Omar, O. ,  Slaymaker, Ian

IPC分类号： C12N15/113 ,  C12N15/10 ,  C12N9/22 ,  C12N15/82

公开(公告)号：EP2921557B1

公开(公告)日：2016-07-13

申请号：EP15154566.2

申请日：2013-12-12

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College

发明人： Zhang, Feng ,  Cong, Le ,  Hsu, Patrick ,  Ran, Fei

IPC分类号： C12N15/63

CPC分类号： C12N15/113 ,  C12N9/22 ,  C12N15/102 ,  C12N15/1082 ,  C12N15/63 ,  C12N15/79 ,  C12N15/907 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2320/11 ,  C12N2320/30 ,  C12N2750/14143 ,  G06F19/18 ,  G06F19/22