公开(公告)号：EP3004128A1

公开(公告)日：2016-04-13

申请号：EP14804387.0

申请日：2014-05-28

申请人： The Coca-Cola Company ,  PureCircle SDN BHD

发明人： PRAKASH, Indra ,  BUNDERS, Cynthia ,  SONI, Pankaj ,  MARKOSYAN, Avetik ,  CYRILLE, Jarrin ,  BADIE, Aurelien ,  HALLE, Rober Ter

IPC分类号： C07H1/06 ,  C12N9/10 ,  C12P19/56

CPC分类号： C12P19/56 ,  A23L2/60 ,  A23L27/33 ,  A23V2002/00 ,  A24B15/10 ,  A24B15/302 ,  C07H15/256 ,  Y02P20/582 ,  A23V2250/258

摘要： Methods of preparing highly purified steviol glycosides, particularly rebaudiosides
A, D and
M are described. The methods include utilizing recombinant microorganisms for converting various staring compositions to target steviol glycosides. In addition, novel steviol glycosides reb
D2 and reb
M2 are disclosed, as are methods of preparing the same. The highly purified rebaudiosides are useful as non-caloric sweetener in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums.

公开(公告)号：EP3004127A1

公开(公告)日：2016-04-13

申请号：EP14804243.5

申请日：2014-05-28

申请人： PureCircle SDN BHD ,  The Coca-Cola Company

发明人： MARKOSYAN, Avetik ,  PRAKASH, Indra ,  BUNDERS, Cynthia ,  SONI, Pankaj ,  CYRILLE, Jarrin ,  BADIE, Aurélien ,  TER HALLE, Robert

IPC分类号： C07H1/06 ,  C12N9/10 ,  C12P19/56

CPC分类号： C12P19/56 ,  A23L2/60 ,  A23L27/33 ,  A23V2002/00 ,  A24B15/10 ,  A24B15/302 ,  C07H15/256 ,  Y02P20/582 ,  A23V2250/258

摘要： Methods of preparing highly purified steviol glycosides, particularly rebaudiosides A, D and M are described. The methods include utilizing recombinant microorganisms for converting various staring compositions to target steviol glycosides. In addition, novel steviol glycosides reb D2 and reb M2 are disclosed, as are methods of preparing the same. The highly purified rebaudiosides are useful as non-caloric sweetener in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums.

摘要翻译： 描述了制备高度纯化的甜菊醇糖苷，特别是瑞鲍迪苷A，D和M的方法。 该方法包括利用重组微生物将各种起始组合物转化成靶甜菊醇糖苷。 此外，公开了新颖的甜菊醇糖苷rebD2和rebM2，以及制备它们的方法。 高度纯化的莱鲍迪甙在食用和咀嚼型组合物如任何饮料，糖果，烘焙产品，饼干和口香糖中用作无热量甜味剂。

公开(公告)号：EP3223621A1

公开(公告)日：2017-10-04

申请号：EP15862653.1

申请日：2015-11-24

申请人： Codexis, Inc. ,  The Coca-Cola Company

发明人： VROOM, Jonathan ,  ALVIZO, Oscar ,  SONI, Pankaj ,  BROWN, Michael D. ,  ENTWISTLE, David

IPC分类号： A23F5/00

CPC分类号： C12P17/182 ,  C07D473/12 ,  C12N9/1007 ,  C12N9/78 ,  C12Y305/04003

摘要： The present invention provides an enzymatic means for the biosynthetic production of caffeine. The present invention provides biosynthetic methods for production of caffeine comprising: providing guanine, a guanine deaminase, at least one methyl transferase, and a methyl donor; contacting the guanine with the gtheuanine deaminase to produce xanthine; contacting the xanthine with the methyl transferase and a methyl donor, under conditions wherein the xanthine is methylated, to produce a monomethylxanthine; contacting the monomethylxanthine with the methyl transferase and a methyl donor, under conditions wherein the monomethylxanthine is methylated, to produce a dimethylxanthine; and contacting the dimethylxanthine with the methyl transferase and a methyl donor, under conditions wherein the dimethylxanthine is methylated, to produce caffeine (i.e., 1,3,7-trimethylxanthine).

摘要翻译： 本发明提供了用于生物合成咖啡因生产的酶手段。 本发明提供了生产咖啡因的生物合成方法，包括：提供鸟嘌呤，鸟嘌呤脱氨酶，至少一种甲基转移酶和甲基供体; 使鸟嘌呤与鸟嘌呤脱氨酶接触以产生黄嘌呤; 在黄嘌呤被甲基化的条件下使黄嘌呤与甲基转移酶和甲基供体接触以产生单甲基黄嘌呤; 在单甲基黄嘌呤被甲基化的条件下，使单甲基黄嘌呤与甲基转移酶和甲基供体接触以产生二甲基黄嘌呤; 并在二甲基黄嘌呤被甲基化的条件下使二甲基黄嘌呤与甲基转移酶和甲基供体接触以产生咖啡因（即1,3,7-三甲基黄嘌呤）。