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1.发明授权Glucanases, nucleic acids encoding them and methods for making and using them 有权葡聚糖酶的核酸，编码这些，以及它们的制备和使用过程
公开(公告)号：EP2404930B1
公开(公告)日：2013-12-18
申请号：EP11176109.4
申请日：2004-07-02
发明人： Steer, Brian , Callen, Walter , Healey, Shaun , Pulliam, Derrick
CPC分类号： C12N9/2405 , A23C9/1322 , A23C19/04 , A23K10/14 , A23K20/189 , A23K40/10 , A23K50/10 , A23L2/52 , A23L29/10 , A23L33/18 , A23V2002/00 , A61K9/0056 , A61K38/47 , C09K8/62 , C11D3/38636 , C12N9/2437 , C12N11/02 , C12N11/14 , C12N11/16 , C12P7/10 , C12P19/02 , C12P19/18 , C12Y302/01004 , D21C1/00 , D21C5/005 , G01N33/53 , G01N33/573 , G01N2333/924 , G01N2500/00 , Y02E50/16 , Y02E50/17
摘要： The invention relates to polypeptides having glucanase, eg endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (eg, endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (eg carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4-bonds in mixed beta-1,3-glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases eg, endoglucanases, mannanases, xylanases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及的具有多肽葡聚糖酶，内切葡聚糖酶例如，甘露聚糖酶，木聚糖酶活性或合成活性的组合，以及多核苷酸编码它们。 在一个方面，葡聚糖酶活性是内切葡聚糖酶活性（例如，内切-1,4-β-D-葡聚糖4-葡聚糖水解酶活性）和在纤维素，纤维素衍生物1,4-β-D-糖苷键的水解包括 如谷物β-D-葡聚糖或含有纤维素部分的木葡聚糖和其它植物材料：在混合的β-1,3-葡聚糖（例如羧甲基纤维素和羟乙基纤维素）地衣淀粉，β-1,4-键。 此外，它们因此，提供设计新的酶和使用方法的方法。 在可选择的方面，新的葡聚糖酶例如内切葡聚糖酶，甘露的NAS，木聚糖酶已经在升高的pH和温度的增加的活性和稳定性。
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2.发明授权Glucanases, nucleic acids encoding them and methods for making and using them 有权葡聚糖酶的核酸，编码这些，以及它们的制备和使用过程
公开(公告)号：EP2404929B1
公开(公告)日：2014-06-18
申请号：EP11176104.5
申请日：2004-07-02
发明人： Steer, Brian , Callen, Walter , Healey, Shaun , Pulliam, Derrick
CPC分类号： C12N9/2405 , A23C9/1322 , A23C19/04 , A23K10/14 , A23K20/189 , A23K40/10 , A23K50/10 , A23L2/52 , A23L29/10 , A23L33/18 , A23V2002/00 , A61K9/0056 , A61K38/47 , C09K8/62 , C11D3/38636 , C12N9/2437 , C12N11/02 , C12N11/14 , C12N11/16 , C12P7/10 , C12P19/02 , C12P19/18 , C12Y302/01004 , D21C1/00 , D21C5/005 , G01N33/53 , G01N33/573 , G01N2333/924 , G01N2500/00 , Y02E50/16 , Y02E50/17
摘要： The invention relates to polypeptides having glucanase, eg endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (eg, endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (eg carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4-bonds in mixed beta-1,3-glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases eg, endoglucanases, mannanases, xylanases have increased activity and stability at increased pH and temperature.
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公开(公告)号：EP2404930A1
公开(公告)日：2012-01-11
申请号：EP11176109.4
申请日：2004-07-02
发明人： Steer, Brian , Callen, Walter , Healey, Shaun , Pulliam, Derrick
CPC分类号： C12N9/2405 , A23C9/1322 , A23C19/04 , A23K10/14 , A23K20/189 , A23K40/10 , A23K50/10 , A23L2/52 , A23L29/10 , A23L33/18 , A23V2002/00 , A61K9/0056 , A61K38/47 , C09K8/62 , C11D3/38636 , C12N9/2437 , C12N11/02 , C12N11/14 , C12N11/16 , C12P7/10 , C12P19/02 , C12P19/18 , C12Y302/01004 , D21C1/00 , D21C5/005 , G01N33/53 , G01N33/573 , G01N2333/924 , G01N2500/00 , Y02E50/16 , Y02E50/17
摘要： The invention relates to polypeptides having glucanase, eg endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (eg, endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (eg carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4-bonds in mixed beta-1,3-glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases eg, endoglucanases, mannanases, xylanases have increased activity and stability at increased pH and temperature.
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4.发明公开Glucanases, nucleic acids encoding them and methods for making and using them 审中-公开葡聚糖酶的核酸，编码这些，以及它们的制备和使用过程
公开(公告)号：EP2404928A1
公开(公告)日：2012-01-11
申请号：EP11176097.1
申请日：2004-07-02
发明人： Steer, Brian , Callen, Walter , Healey, Shaun , Pulliam, Derrick
CPC分类号： C12N9/2405 , A23C9/1322 , A23C19/04 , A23K10/14 , A23K20/189 , A23K40/10 , A23K50/10 , A23L2/52 , A23L29/10 , A23L33/18 , A23V2002/00 , A61K9/0056 , A61K38/47 , C09K8/62 , C11D3/38636 , C12N9/2437 , C12N11/02 , C12N11/14 , C12N11/16 , C12P7/10 , C12P19/02 , C12P19/18 , C12Y302/01004 , D21C1/00 , D21C5/005 , G01N33/53 , G01N33/573 , G01N2333/924 , G01N2500/00 , Y02E50/16 , Y02E50/17
摘要： The invention relates to polypeptides having glucanase, eg endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (eg, endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (eg carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4-bonds in mixed beta-1,3-glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases eg, endoglucanases, mannanases, xylanases have increased activity and stability at increased pH and temperature.
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5.发明公开Glucanases, nucleic acids encoding them and methods for making and using them 审中-公开葡聚糖酶，编码它们的核酸以及制备和使用它们的方法
公开(公告)号：EP2409981A1
公开(公告)日：2012-01-25
申请号：EP11176116.9
申请日：2004-07-02
发明人： Steer, Brian , Callen, Walter , Healey, Shaun , Pulliam, Derrick
CPC分类号： C12N9/2405 , A23C9/1322 , A23C19/04 , A23K10/14 , A23K20/189 , A23K40/10 , A23K50/10 , A23L2/52 , A23L29/10 , A23L33/18 , A23V2002/00 , A61K9/0056 , A61K38/47 , C09K8/62 , C11D3/38636 , C12N9/2437 , C12N11/02 , C12N11/14 , C12N11/16 , C12P7/10 , C12P19/02 , C12P19/18 , C12Y302/01004 , D21C1/00 , D21C5/005 , G01N33/53 , G01N33/573 , G01N2333/924 , G01N2500/00 , Y02E50/16 , Y02E50/17
摘要： The invention relates to polypeptides having glucanase, eg endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (eg, endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (eg carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4-bonds in mixed beta-1,3-glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases eg, endoglucanases, mannanases, xylanases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及具有葡聚糖酶（例如内切葡聚糖酶，甘露聚糖酶，木聚糖酶活性或这些活性的组合）的多肽以及编码它们的多核苷酸。 在一个方面，葡聚糖酶活性是内切葡聚糖酶活性（例如，内-1,4-β-D-葡聚糖4-葡聚糖水解酶活性）并且包括纤维素中的1,4-β-D-糖苷键的水解，纤维素衍生物 （例如羧甲基纤维素和羟乙基纤维素）地衣蛋白，混合β-1,3-葡聚糖（例如谷类β-D-葡聚糖或木葡聚糖）和其他含有纤维素部分的植物材料中的β-1,4-键。 此外，还提供了设计新酶的方法及其使用方法。 在替代方面，新葡聚糖酶例如内切葡聚糖酶，甘露聚糖酶，木聚糖酶在增加的pH和温度下具有增加的活性和稳定性。
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公开(公告)号：EP2404929A1
公开(公告)日：2012-01-11
申请号：EP11176104.5
申请日：2004-07-02
发明人： Steer, Brian , Callen, Walter , Healey, Shaun , Pulliam, Derrick
CPC分类号： C12N9/2405 , A23C9/1322 , A23C19/04 , A23K10/14 , A23K20/189 , A23K40/10 , A23K50/10 , A23L2/52 , A23L29/10 , A23L33/18 , A23V2002/00 , A61K9/0056 , A61K38/47 , C09K8/62 , C11D3/38636 , C12N9/2437 , C12N11/02 , C12N11/14 , C12N11/16 , C12P7/10 , C12P19/02 , C12P19/18 , C12Y302/01004 , D21C1/00 , D21C5/005 , G01N33/53 , G01N33/573 , G01N2333/924 , G01N2500/00 , Y02E50/16 , Y02E50/17
摘要： The invention relates to polypeptides having glucanase, eg endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (eg, endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (eg carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4-bonds in mixed beta-1,3-glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases eg, endoglucanases, mannanases, xylanases have increased activity and stability at increased pH and temperature.
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公开(公告)号：EP2404931A1
公开(公告)日：2012-01-11
申请号：EP11176111.0
申请日：2004-07-02
发明人： Steer, Brian , Callen, Walter , Healey, Shaun , Pulliam, Derrick
CPC分类号： C12N9/2405 , A23C9/1322 , A23C19/04 , A23K10/14 , A23K20/189 , A23K40/10 , A23K50/10 , A23L2/52 , A23L29/10 , A23L33/18 , A23V2002/00 , A61K9/0056 , A61K38/47 , C09K8/62 , C11D3/38636 , C12N9/2437 , C12N11/02 , C12N11/14 , C12N11/16 , C12P7/10 , C12P19/02 , C12P19/18 , C12Y302/01004 , D21C1/00 , D21C5/005 , G01N33/53 , G01N33/573 , G01N2333/924 , G01N2500/00 , Y02E50/16 , Y02E50/17
摘要： The invention relates to polypeptides having glucanase, eg endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (eg, endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (eg carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4-bonds in mixed beta-1,3-glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases eg, endoglucanases, mannanases, xylanases have increased activity and stability at increased pH and temperature.
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8.发明授权Xylanases, nucleic acids encoding them and methods for making and using them 有权木聚糖酶，编码它们的核酸以及制备和使用它们的方法
公开(公告)号：EP2298904B1
公开(公告)日：2017-05-24
申请号：EP10179325.5
申请日：2003-06-16
发明人： Steer, Brian , Callen, Walter , Healey, Shaun , Hazlewood, Geoff , Wu, Di , Blum, David , Esteghlalian, Alireza
CPC分类号： C12N9/248 , A01K2217/05 , C07K2319/00 , C07K2319/02 , C12N9/2434 , C12N9/2482 , C12P19/14 , C12Y302/01008 , D21C5/005 , D21H17/005 , Y02E50/16 , Y02E50/17
摘要： Xylanases, Nucleic Acids Encoding Them and Methods for Making and Using Them The invention relates to xylanases and to polynucleotides encoding the xylanases. In addition, methods of designing new xylanases and methods of use are also provided. The xylanases have increased activity and stability at increased pH and temperature.
摘要翻译： 木聚糖酶，编码它们的核酸以及制备和使用它们的方法本发明涉及木聚糖酶和编码木聚糖酶的多核苷酸。 此外，还提供了设计新型木聚糖酶的方法和使用方法。 木聚糖酶在增加的pH和温度下具有增加的活性和稳定性。
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9.发明公开Xylanases, nucleic acids encoding them and methods for making and using them 有权木聚糖酶的核酸，编码这些，以及它们的制备和使用过程
公开(公告)号：EP2298904A1
公开(公告)日：2011-03-23
申请号：EP10179325.5
申请日：2003-06-16
申请人： Verenium Corporation
发明人： Steer, Brian , Callen, Walter , Healey, Shaun , Hazlewood, Geoff , Wu, Di , Blum, David , Esteghlalian, Alireza
CPC分类号： C12N9/248 , A01K2217/05 , C07K2319/00 , C07K2319/02 , C12N9/2434 , C12N9/2482 , C12P19/14 , C12Y302/01008 , D21C5/005 , D21H17/005 , Y02E50/16 , Y02E50/17
摘要： The invention relates to xylanases and to polynucleotides encoding the xylanases. In addition, methods of designing new xylanases and methods of use are also provided. The xylanases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及木聚糖酶和多核苷酸编码木聚糖酶。 此外，因此，提供了设计新的木聚糖酶和使用方法的方法。 木聚糖酶已经在升高的pH和温度的增加的活性和稳定性。
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10.发明公开Xylanases, nucleic acids encoding them and methods for making and using them 审中-公开木聚糖酶的核酸，编码这些，以及它们的制备和使用过程
公开(公告)号：EP2548956A1
公开(公告)日：2013-01-23
申请号：EP12168130.8
申请日：2007-02-14
申请人： Verenium Corporation
发明人： Weiner, David , Hansen, Connie Jo , Hancock, Charles , Woodward, Thomas , Beaver, Scott W , Blum, David , Healey, Shaun , Varvak, Alexander , Todaro, Thomas , Hazlewood, Geoff , Chang, Kristine , Chang, Hwai , Desantis, Grace
IPC分类号： C12N9/24
CPC分类号： C12N9/248 , A23K10/12 , A23K20/163 , A23L5/00 , A23L7/00 , A23L29/30 , A23L31/00 , A23L31/10 , A23L33/125 , A23V2002/00 , C12N9/2405 , C12N9/2437 , C12N9/2482 , C12N9/2488 , C12N9/2494 , C12P19/02 , C12P19/14 , C12Y302/01008 , D06L4/40 , D06L4/70 , D21C3/003 , D21C9/10 , D21H17/005 , Y02E50/16
摘要： The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal β-1,4-xylosidic linkages or endo- β-1,4-glucanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.
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