公开(公告)号：EP0558632B1

公开(公告)日：2000-08-16

申请号：EP92901079.1

申请日：1991-11-22

申请人： COULTER CORPORATION

发明人： RUSSELL, Thomas ,  HUDSON, James, Carey ,  COULTER, Wallace, H. ,  RODRIGUEZ, Carlos, M. ,  HAJEK, Constance, Mary

IPC分类号： C12M1/34 ,  G01N33/49 ,  G01N33/50 ,  G01N33/569

CPC分类号： G01N33/56972 ,  Y10S435/808 ,  Y10S435/962 ,  Y10S436/805 ,  Y10S436/824 ,  Y10S436/825

摘要： A method and apparatus for performing screening of one or more cell groups of interest obscured by a cell population utilizing at least one light sensing parameter. The cell group of interest such as a subset of a white blood cell population is enumerated by utilizing microspheres having monoclonal antibodies bound thereto to modify the sensed characteristics of specified cells to differentiate the cell group of interest from the obscuring cell population. A whole blood sample or portion thereof also can be measured first, then have specified cells deleted therefrom to enable the cell group of interest to be sensed in an area in which it would otherwise have been obscured by the deleted cells. The sample portion is again measured and compared to the first measurement to differentiate the cell group of interest.

公开(公告)号：EP0627080B1

公开(公告)日：2000-04-05

申请号：EP93905022.5

申请日：1993-02-11

申请人： COULTER CORPORATION

发明人： SHENKIN, Mark, L. ,  HAMELIK, Ronald, M. ,  WOOD, James, C., S.

IPC分类号： G01N33/48 ,  G01N33/50 ,  G01N33/577 ,  G01N33/58 ,  G01N15/14 ,  G01N1/30 ,  G01N33/569

CPC分类号： G01N33/5094 ,  G01N33/56972 ,  G01N2015/1477 ,  G01N2015/1486

摘要： A method is provided to ascertain the total number of cells per unit volume of a cell specimen in an apparatus which does not measure the volume of a suspension containing the specimen as it is being analyzed. The method comprising mixing a suspension of a known quantity of particles having a first light scatter signal with a known volume of a cell specimen having a second light scatter signal different from the first signal to obtain a suspension having a concentration of particles per specimen volume, passing each of said particles and each cell in said cell specimen, in turn, through a light beam, each of said particles and cells producing at least one forward light scattering pattern, counting the number of cells and the number of particles in a portion of said suspension, and determining the total number of cells per unit volume of specimen.

公开(公告)号：EP0760940B1

公开(公告)日：1998-06-24

申请号：EP95920373.8

申请日：1995-05-24

申请人： COULTER CORPORATION

发明人： CALVO, Manuel ,  PARKER, Nicholas ,  HOSKINSON, James ,  KETANT, Etzer ,  CHRISTOU, Kyriakos

IPC分类号： G01N1/00 ,  G01N35/10

CPC分类号： G01N35/1079 ,  Y10T436/114165 ,  Y10T436/114998 ,  Y10T436/2575

摘要： A universal stripper plate (40) for operating on a plurality of different types of collection containers or tubes (20). The universal stripper plate includes a universal button (42) for aligning and seating the various tube stoppers or caps (26) for piercing. The universal button is mounted onto a stripper plate, which can be driven into the tube seating/alignment, tube piercing and aspiration and tube release positions. The universal stripper plate also can include a bar code tab (76) to provide a bar code reference and a piercing needle alignment for the various tubes.

公开(公告)号：EP0756709A1

公开(公告)日：1997-02-05

申请号：EP95919112.0

申请日：1995-04-11

申请人： COULTER CORPORATION

发明人： COULTER, Wallace, H. ,  ZWERNER, Robert, K. ,  SCHMITTLING, Robert, J. ,  RUSSELL, Thomas, R.

IPC分类号： B01D21 ,  G01N33

CPC分类号： G01N33/54333 ,  G01N33/553 ,  G01N33/56972 ,  G01N33/574 ,  Y10S436/824

摘要： A separation procedure for separating a selected desired or undesired population from a biological sample utilizing relatively heavy, dense particles and gravity sedimentation. The particles have one or more reactants bound thereto which are specific to and will bind with the selected population. The particles preferably are mixed with the sample by repeatedly causing the particles to settle through a substantial portion of the sample to bind to the selected population. The particles with the bound selected population then are allowed to preferentially settle in the sample and the supernatant including the non-selected population is separated from the particles with the selected population bound thereto. The particles can be heated for sterilization and endotoxin removal.

公开(公告)号：EP0701707A1

公开(公告)日：1996-03-20

申请号：EP94918134.0

申请日：1994-05-26

申请人： COULTER CORPORATION

发明人： SCHMITZ, Brian, Donald ,  BOTT, Steven, Eric ,  HART, William, Horward

IPC分类号： G01B11 ,  G01N15 ,  G02B27

CPC分类号： G01N15/0211 ,  G02B27/46

摘要： A fiber optic spatial filter assembly for laser diffraction particle sizing apparatus utilizing a laser to generate a monochromatic light beam which is coupled to an optical fiber (36) operating substantially in a monomode and creating a beam of light (56) having a high degree of spatial coherence which is then passed through collimating lenses (64, 66) to interrogate and impinge upon the particles of matter (86) through which the laser diffracted light passes. The light scattered by the particles is focused onto a Fourier plane (96) and thereafter impinges upon a photooptical detector array (98), positioned coincident with the Fourier plane, for measuring the light intensities of the scattered light by scattering angle, thus enabling the computation of particle size.

公开(公告)号：EP0681606A1

公开(公告)日：1995-11-15

申请号：EP94907284.0

申请日：1994-01-19

申请人： COULTER CORPORATION

发明人： SARALEGUI, Francisco, J. ,  SCHLINKMANN, Alex, W.

IPC分类号： G01N33 ,  B01F11 ,  C12M1 ,  G01N1 ,  G01N15 ,  G01N35 ,  G01N21

CPC分类号： G01N35/025 ,  B01F11/0014 ,  B01F11/0037 ,  G01N21/253 ,  G01N2035/00524 ,  G01N2035/041

摘要： An apparatus (10) which provides an automatic, signal controlled, sample mixing/resuspending, aspiration and delivery system; wherein a demountable, rotatable carousel (20) temporarily holds a multiplicity of sample containers (22). Electromechanical means (86, 88, 90), including optical sensors (100, 106), is programmed to automatically move the carousel to a pre-selected position. A self centering vortexer/mixer (28) lifts a selected sample container from the carousel, sealingly engages the sample container within a sample container support (32) and thereafter orbitally mixes and resuspends the sample container contents. An aspiration probe (214) enters the sample container through the support to receive the sample, which is forced out of the sample container by means of compressed air introduced into the sample container via the sample container support. The sample is delivered to an operably associated flow cytometer (13) via the aspiration probe, after which the probe is washed and readied for the next cycle of operation.

公开(公告)号：EP0665955A1

公开(公告)日：1995-08-09

申请号：EP93923887.0

申请日：1993-10-14

申请人： COULTER CORPORATION

发明人： SIIMAN, Olavi ,  BURSHTEYN, Alexander ,  GUPTA, Ravinder, K.

IPC分类号： G01N33 ,  A61K39 ,  B01J13 ,  B03C1 ,  C07K14 ,  C07K16 ,  C07K17 ,  C07K19 ,  C12N11

CPC分类号： G01N33/54313 ,  B03C1/01 ,  G01N33/531 ,  G01N33/5434 ,  G01N33/54393 ,  G01N2446/20 ,  G01N2446/30 ,  G01N2446/84 ,  G01N2446/86

摘要： Gelatin and aminodextran coated particles useful in immunoassays and methods of making the same are disclosed. The preparation of aminodextrans having varying 3.3-16.5 % of starting dextran sugar groups substituted by amine groups is described, as is also a method of crosslinking gelatin and aminodextran without the use of a stabilizer. The core substance of the coated particles may be a magnetic substance having a gelatin/aminodextran coating or a polymeric substance, either magnetic or non-magnetic, having either a gelatin/aminodextran coating or an aminodextran coating.

公开(公告)号：EP0532684B1

公开(公告)日：1995-08-09

申请号：EP91912637.5

申请日：1991-05-31

申请人： COULTER CORPORATION

发明人： SIIMAN, Olavi ,  BURSHTEYN, Alexander

IPC分类号： H01F1/26 ,  B01F3/00 ,  B01J13/02 ,  B32B5/16 ,  H01F1/37 ,  B03C1/00

CPC分类号： B01J13/0013 ,  B03C1/01 ,  H01F1/061 ,  H01F1/445 ,  Y10T428/2991

摘要： A method is described for the preparation of uniform colloidal particles of ferrites, containing manganese(II), zinc(II), iron(II), barium(II), cobalt(II) or nickel(II), or a mixture of manganese(II) and zinc(II), at a relatively low temperature in the presence of a gelatin solution which acts as a support vehicle for the nucleation and growth of colloidal particles of metal oxide and for dispersion as separate single particles.

公开(公告)号：EP0386136B1

公开(公告)日：1995-03-22

申请号：EP89900430.3

申请日：1988-10-24

申请人： COULTER CORPORATION

发明人： KORTRIGHT, Kenneth, H. ,  HOFHEINZ, David, E. ,  FORMAN, Meryl, Ann ,  LEE, Song, Yea ,  SMARIGA, Paulette, Elizabeth ,  STONER, Candie, Sue

IPC分类号： G01N33/577 ,  C12Q1/70

CPC分类号： G01N33/54306 ,  C07K16/1054 ,  G01N33/56988 ,  Y10S435/974 ,  Y10S436/80 ,  Y10S436/805 ,  Y10S436/808 ,  Y10S436/809 ,  Y10S436/811 ,  Y10S436/825 ,  Y10S436/826

摘要： A solid-phase immunoassay is provided for determination of HIV antigens in human physiological fluid. The immunoassay is characterized by the coating of a solid substrate with a unique monoclonal antibody which recognizes a common antigenic determinant of a group of HIV core antigens and no HIV envelope antigens of HIV. The test sample preferably also is subjected to a lysing reagent prior to the incubation for uniformly dispersing antigens which may be present in the test sample.

公开(公告)号：EP0454691B1

公开(公告)日：1995-01-18

申请号：EP90901199.1

申请日：1989-11-17

申请人： COULTER CORPORATION

发明人： CABRERA, Pedro, Pablo ,  ESTOQUE, Daniel, A.

IPC分类号： G01N1/00 ,  G01N1/28

CPC分类号： G01N1/38 ,  G01N15/10 ,  G01N35/1097

摘要： A liquid metering and transfer valve assembly (10) for a diluting system which includes a pair of stationary outer valve disc elements (12, 16) sandwiching a central rotatable valve disc element (14). The elements (12, 14, 16) are coaxially aligned and mounted on a spindle (24). One (16) of the outer elements (12, 16) has a first external loop (28) secured thereto, said loop (28) having a precise interior volume. An aspirator probe (26) is secured to the central element (14). The central element (14) carries an axial segmenting passageway (72) having a precise interior volume. A second external loop (86) is secured to the central element (14). The second loop (86) extends outwardly, preferably radially, of said element (14) and has a precise interior volume. Appropriate interior passageways (36, 54, 56, 30) are provided in the outer elements (12, 16) for directing a liquid sample in a continuous path through the valve assembly, the path including the one segmenting passageway (72) and the first and second loops (28, 86). Additional interior passageways (58, 34, 62, 76, 74, 82, 84) are provided for coupling the valve assembly to sources of diluent P2 and to exterior destinations. The central element (14) is rotated to isolate the contents of the segmenting passageway (72) and of the first and the second loops (28, 86) and diluent is fed thereto to drive the isolated contents thereof to said destinations. Only a single loading step is required to obtain three precise volumes of liquid sample. The valve assembly can be rinsed and continuous internal channels (102, 104) are provided for preventing any liquid from reaching the circumferential surfaces 12'', 14'', 16'' of the valve assembly.