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    METHOD FOR AMPLIFYING NUCLEIC ACID SEQUENCE
    3.
    发明公开
    METHOD FOR AMPLIFYING NUCLEIC ACID SEQUENCE 有权
    Verfahren zur Amplifizierung einerNukleinsäuresequenzunter Verwendung eineschimärenPrimers

    公开(公告)号：EP1167524A1

    公开(公告)日：2002-01-02

    申请号：EP00908056.5

    申请日：2000-03-14

    申请人： Takara Shuzo Co, Ltd.

    发明人： MUKAI, Hiroyuki ,  YAMAMOTO, Junko ,  TAKEDA, Osamu ,  MIYAKE, Kazue ,  UEMORI, Takashi ,  SATO, Yoshimi ,  MORIYAMA, Mariko ,  SAWARAGI, Haruhisa ,  HAGIYA, Michio ,  ASADA, Kiyozo ,  KATO, Ikunoshin

    IPC分类号： C12N15/10 ,  C12Q1/68

    CPC分类号： C12Q1/6844 ,  C12Q2531/119 ,  C12Q2525/121 ,  C12Q2521/319

    摘要： A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.

    摘要翻译： 一种用于扩增核酸序列的方法和方法，其特征在于在嵌合寡核苷酸引物存在下进行DNA合成反应; 提供大量DNA扩增片段的方法; 通过将上述方法与另一种核酸序列扩增方法结合来​​扩增核酸序列的有效方法; 用于检测用于检测或定量微生物如病毒，细菌，真菌或酵母的核酸序列的方法; 以及通过上述方法原位检测DNA扩增片段的方法。

    REMEDIES OR PREVENTIVES CONTAINING CYCLOPENTENONE COMPOUNDS AS THE ACTIVE INGREDIENT
    7.
    发明公开
    REMEDIES OR PREVENTIVES CONTAINING CYCLOPENTENONE COMPOUNDS AS THE ACTIVE INGREDIENT 有权
    HEILMITTEL UND VORBEUGENDE MITTEL DIE CYCLOPENTENONVERBINDUNGEN ALS WIRKSAMEN STOFF ENTHALTEN

    公开(公告)号：EP1112742A1

    公开(公告)日：2001-07-04

    申请号：EP99937010.9

    申请日：1999-08-10

    申请人： Takara Shuzo Co, Ltd.

    发明人： KOBAYASHI, Eiji ,  OHNOGI, Hiromu, A-406, Takarashuzoshataku ,  SAGAWA, Hiroaki, 503, Hamoparesu-Kusatsu ,  TOMINAGA, Takanari ,  NISHIYAMA, Eiji ,  KOYAMA, Nobuto ,  IKAI, Katsushige ,  KATO, Ikunoshin

    IPC分类号： A61K31/215 ,  A61K31/22

    CPC分类号： A61K31/215 ,  A61K31/22 ,  C07C69/013 ,  C07C2601/10 ,  Y02A50/411

    摘要： Remedies or preventives for diseases with a need for immunoregulation, diseases with a need for inhibition of inflammation, diseases with a need for regulation of tumor necrosis factor production, diseases with a need for regulation of fungal growth, diseases with a need for regulation of cell adhesion or disease with a deed for induction of heat-shock protein, which contain as the active ingredient at least one compound selected from among cyclopentenone derivatives represented by general formula [I], optically active isomers and salts thereof, wherein R 1 and R 2 are the same or different and each represents hydrogen, an aliphatic group, an aromatic group or an aromatic aliphatic group.

    摘要翻译： 需要免疫调节的疾病的治疗或预防措施，需要抑制炎症的疾病，需要调节肿瘤坏死因子产生的疾病，需要调节真菌生长的疾病，需要的疾病 含有诱导热休克蛋白的细胞的细胞粘附或疾病的调节，其含有作为活性成分的至少一种选自通式Ⅳ所示的环戊烯酮衍生物的化合物，其旋光异构体及其盐，其中R 1和R 2为 相同或不同，各自表示氢，脂族基，芳基或芳族脂族基。

    METHOD FOR SYNTHESIZING DNA
    8.
    发明公开
    METHOD FOR SYNTHESIZING DNA 审中-公开
    PCR-METHODE ZUR DNA-SYNTHESE UNTER VERWENDUNG VON SPERGUALIN

    公开(公告)号：EP1111044A1

    公开(公告)日：2001-06-27

    申请号：EP99940673.9

    申请日：1999-09-06

    申请人： Takara Shuzo Co, Ltd.

    发明人： UEMORI, Takashi ,  SATO, Yoshimi ,  OKAWA, Mariko ,  FUJITA, Tomoko ,  MIYAKE, Kazue ,  TAKEDA, Osamu ,  SAGAWA, Hiroaki ,  HAGIYA, Michio ,  MUKAI, Hiroyuki ,  ASADA, Kiyozo ,  KATO, Ikunoshin

    IPC分类号： C12N15/11 ,  C12Q1/68 ,  G01N33/53

    CPC分类号： C12N15/1003 ,  C12Q1/686 ,  C12Q2527/125

    摘要： A DNA synthesis method with a shortened time period required for DNA synthesis by polymerase chain reaction (PCR), characterized in that a DNA polymerase is used in an amount effective for providing more than 10 ng of amplified DNA fragments of about 2 kb per 50 µl of a reaction mixture, when PCR is carried out under the following conditions (A) and (B): (A) reaction mixture: 50 µl volume of a reaction mixture comprising DNA polymerase, 1 ng of genomic DNA from Escherichia coli, and 10 pmol each of primers Eco-1 and Eco-2 (nucleotide sequences of the primers Eco-1 and Eco-2 being shown in SEQ ID NOs: 10 and 11 of Sequence Listing, respectively); and having a composition suitable for the DNA polymerase; and (B) reaction conditions: 35 cycles of PCR, wherein one cycle consists of 99°C, 1 second - 66°C, 7 seconds; a kit for DNA synthesis usable for the DNA synthesis method; and an article of manufacture of a PCR agent. According to the present invention, the procedures in the genetic engineering studies and industries involved with PCR can be speeded up.

    摘要翻译： 一种通过聚合酶链式反应（PCR）进行DNA合成所需的时间缩短的DNA合成方法，其特征在于使用DNA聚合酶，其用量可提供超过10ng的扩增DNA片段，每50ul约2kb （A）和（B）：（A）反应混合物：50μl体积的包含DNA聚合酶的反应混合物，1ng来自大肠杆菌的基因组DNA， 和引物Eco-1和Eco-2（引物Eco-1和Eco-2的核苷酸序列分别示于序列表的SEQ ID NO：10和11中）的10pmol; 并具有适合于DNA聚合酶的组合物; 和（B）反应条件：35个循环的PCR，其中一个循环由99℃，1秒-66℃，7秒; 用于DNA合成方法的DNA合成试剂盒; 和PCR试剂的制造。 根据本发明，可以加快基因工程研究中涉及PCR的工艺步骤。