利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

124 条记录 (耗时 0.023 秒)

    METHOD FOR SEPARATING BIOTINYLATED NUCLEIC ACID
    2.
    发明公开
    METHOD FOR SEPARATING BIOTINYLATED NUCLEIC ACID 审中-公开

    公开(公告)号：EP3239710A4

    公开(公告)日：2018-05-23

    申请号：EP15872907

    申请日：2015-12-17

    申请人： WAKO PURE CHEM IND LTD

    发明人： UKEKAWA RYO

    IPC分类号： G01N33/53 ,  C07K14/375 ,  C07K17/14 ,  C12Q1/6806

    CPC分类号： G01N33/53 ,  C07K14/375 ,  C07K17/14 ,  C12Q1/68 ,  C12Q1/6806

    摘要： An object of the present invention is to obtain a biotinylated nucleic acid efficiently, by enhancing dissociation efficiency of biotin in the biotinylated nucleic acid and tamavidin 2 in the tamavidin 2-immobilized insoluble carrier, and the present invention relates to "A method for separating a biotinylated nucleic acid, comprising the following steps: (1) a step for contacting a sample containing a biotinylated nucleic acid wherein the biotin is bound to the nucleic acid with a insoluble carrier on which tamavidin is immobilized (a tamavidin-immobilized insoluble carrier) to form a complex of the biotinylated nucleic acid and the tamavidin-immobilized insoluble carrier (step A-1), (2) a step for separating the biotinylated nucleic acid from the complex obtained in the step A-1, in a solution having pH of 7.8 to 9.5, and in the presence of free biotin (step A-2), a method for separating the biotinylated nucleic acid to which the nucleic acid-binding protein is bound, a method for separating the nucleic acid-binding protein, and a kit for separating the nucleic acid.

    METHOD FOR SEPARATING BIOTINYLATED NUCLEIC ACID
    4.
    发明公开
    METHOD FOR SEPARATING BIOTINYLATED NUCLEIC ACID 审中-公开
    分离生物碱化核酸的方法

    公开(公告)号：EP3239710A1

    公开(公告)日：2017-11-01

    申请号：EP15872907.9

    申请日：2015-12-17

    申请人： Wako Pure Chemical Industries, Ltd.

    发明人： UKEKAWA Ryo

    IPC分类号： G01N33/53 ,  C07K14/375 ,  C07K17/14 ,  C12Q1/68

    CPC分类号： G01N33/53 ,  C07K14/375 ,  C07K17/14 ,  C12Q1/68 ,  C12Q1/6806

    摘要： An object of the present invention is to obtain a biotinylated nucleic acid efficiently, by enhancing dissociation efficiency of biotin in the biotinylated nucleic acid and tamavidin 2 in the tamavidin 2-immobilized insoluble carrier, and the present invention relates to "A method for separating a biotinylated nucleic acid, comprising the following steps: (1) a step for contacting a sample containing a biotinylated nucleic acid wherein the biotin is bound to the nucleic acid with a insoluble carrier on which tamavidin is immobilized (a tamavidin-immobilized insoluble carrier) to form a complex of the biotinylated nucleic acid and the tamavidin-immobilized insoluble carrier (step A-1), (2) a step for separating the biotinylated nucleic acid from the complex obtained in the step A-1, in a solution having pH of 7.8 to 9.5, and in the presence of free biotin (step A-2), a method for separating the biotinylated nucleic acid to which the nucleic acid-binding protein is bound, a method for separating the nucleic acid-binding protein, and a kit for separating the nucleic acid.

    摘要翻译： 本发明的一个目的是通过增强生物素化核酸和tamavidin 2固定的不溶性载体中生物素化的核酸和tamavidin 2中的生物素的解离效率而有效地获得生物素化的核酸，并且本发明涉及“ （1）使含有生物素化核酸的生物素结合于核酸的样品与固定了tamavidin的不溶性载体（tamavidin固定化不溶性载体）的样品接触至生物素化核酸的步骤 形成生物素化核酸和tamavidin固定化不溶性载体的复合体（步骤A-1），（2）从步骤A-1获得的复合物中分离生物素化核酸的步骤， 7.8至9.5，并且在存在游离生物素的情况下（步骤A-2），分离核酸结合蛋白所结合的生物素化核酸的方法， 对核酸结合蛋白进行鉴定，以及用于分离核酸的试剂盒。

    HERICIUM ERINACEUM-DERIVED LECTIN SPECIFIC FOR SIALIC ACID LINKAGE
    5.
    发明公开
    HERICIUM ERINACEUM-DERIVED LECTIN SPECIFIC FOR SIALIC ACID LINKAGE 有权
    FÜRSIALINSÄUREBINDUNGSPEZIFISCHES LECTIN AUS HERICIUM ERINACEUM

    公开(公告)号：EP3072954A4

    公开(公告)日：2016-10-19

    申请号：EP14862325

    申请日：2014-11-14

    申请人： KOREA RES INST OF BIOSCIENCE

    发明人： KIM SEONGHUN

    IPC分类号： C12N1/14 ,  C07K14/37 ,  C07K14/42

    CPC分类号： C07K14/375 ,  C07K14/37 ,  G01N33/582 ,  G01N33/6848 ,  G01N2333/42

    摘要： The present invention concerns lectins isolated from the fruiting body of a novel Hericium erinaceum (deposit number: KCTC 12499BP) NEU-1 L strain which bind specifically to sialic acid. The invention further pertains to uses of such lectinsn abd to processes for their preparation thereby. The lectin of the present invention can be useful as an active ingredient of a composition or a kit for measuring or detecting glycoproteins, glycopeptides, glycolipids, sugar precursors or oligosaccharides containing sialic acid moieties, or further for measuring or detecting cell lines, bacteria and viruses containing sialoglycoconjugates.

    摘要翻译： 本发明涉及从与唾液酸特异性结合的新型赫氏酵母（保藏号：KCTC 12499BP）NEU-1L菌株的子实体分离的凝集素。 本发明进一步涉及这种凝集素abd的用途，因此其制备方法。 本发明的凝集素可用作用于测量或检测糖蛋白，糖肽，糖脂，糖前体或含有唾液酸部分的寡糖或进一步用于测量或检测细胞系，细菌和病毒的组合物或试剂盒的活性成分 含有sialoglycoconjugates。

    AGROCYBE AEGERITA LECTIN AAL-2, AND ENCODING GENE THEREOF, PREPARATION METHOD THEREFOR AND APPLICATION THEREOF
    6.
    发明公开
    AGROCYBE AEGERITA LECTIN AAL-2, AND ENCODING GENE THEREOF, PREPARATION METHOD THEREFOR AND APPLICATION THEREOF 有权
    AGROCYBE AEGERITA LEKTIN AAL-2，UND DAVON KODIERENDE GEN，VORBEREITUNG VERFAHRENDAFÜRUND ANWENDUNG DAVON

    公开(公告)号：EP2740739A4

    公开(公告)日：2015-03-04

    申请号：EP11870457

    申请日：2011-10-24

    申请人： WUHAN ALPHA OMEGA BIOLOGY TECHNOLOGY CO LTD

    发明人： SUN HUI ,  JIANG SHUAI ,  GU BIANLI ,  CHEN YIJIE ,  YU GUOJUN ,  YIN YALIN ,  ZHANG JINLIN

    IPC分类号： C07K14/375 ,  A61K38/16 ,  A61P35/00 ,  C07K1/22 ,  C12N1/00 ,  C12N15/31 ,  C12N15/63 ,  G01N33/50

    CPC分类号： C07K14/375 ,  A61K38/00 ,  G01N33/57438 ,  G01N2333/4724

    摘要： The present invention provides the Agrocybe aegerita lectin AAL-2, its encoding gene, the preparation methods and its uses. The invention uses the affinity chromatography separated the lectin protein AAL-2 from the Agrocybe aegerita total protein, of which the amino acid sequence is set forth in SEQ ID NO: 2 and the nucleotide sequence encoding the said protein is set forth in SEQ ID NO: 1. Cell tests indicate that the Agrocybe aegerita lectin AAL-2 isolated in the invention has excellent inhibition activity to the tumor cells, which can induce apoptosis of hepatoma cells, significantly. Animal experiments show that AAL-2 has a better therapeutic effect on tumor. The glycochip technology test results show that the AAL-2 preferentially binds to the carbohydrate or glycoproteins with the N-Acetylglucosamine as the terminal, which can be used as a reagent for testing the N-Acetylglucosamine relevant diseases or the N-Acetylglucosamine relevant carbohydrate structure.

    摘要翻译： 本发明提供了Agrocybe aegerita凝集素AAL-2，其编码基因，其制备方法及其用途。 本发明使用从Agrocybe aegerita总蛋白中分离凝集素蛋白AAL-2的亲和层析，其氨基酸序列如SEQ ID NO：2所示，编码所述蛋白的核苷酸序列列于SEQ ID NO 1.细胞试验表明，本发明中分离出的Agrocybe aegerita凝集素AAL-2对肿瘤细胞具有优异的抑制活性，可显着诱导肝癌细胞凋亡。 动物实验表明，AAL-2对肿瘤有较好的治疗作用。 糖芯片技术测试结果表明，AAL-2以N-乙酰基葡萄糖胺为末端优先与碳水化合物或糖蛋白结合，可用作测试N-乙酰葡糖胺相关疾病或N-乙酰葡糖胺相关碳水化合物结构的试剂 。

    MODIFIED BIOTIN-CONJUGATED PROTEIN
    8.
    发明公开
    MODIFIED BIOTIN-CONJUGATED PROTEIN 有权
    改良生物素康酮蛋白

    公开(公告)号：EP2447364A1

    公开(公告)日：2012-05-02

    申请号：EP09846508.1

    申请日：2009-06-24

    申请人： Japan Tobacco, Inc.

    发明人： TAKAKURA, Yoshimitsu ,  TSUNASHIMA, Masako ,  SOFUKU, Kozue

    IPC分类号： C12N15/09 ,  C07K14/37 ,  C07K17/00

    CPC分类号： C07K14/375 ,  C07K2319/22 ,  G01N2333/36

    摘要： The present invention provides a modified biotin-binding protein comprising an amino acid sequence represented by SEQ ID NO: 2 or its modified sequence and having a biotin-binding activity and replacement selected from the group consisting of:
    1) replacement of the 36th serine residue of SEQ ID NO: 2 with an amino acid residue that does not form a hydrogen bond;
    2) replacement of the 80th tryptophan residue of SEQ ID NO: 2 with a hydrophilic amino acid residue;
    3) replacement of the 116th aspartic acid residue of SEQ ID NO: 2 with an amino acid residue that does not form a hydrogen bond;
    4) replacement of the 46th proline residue of SEQ ID NO: 2 with a threonine, serine, or tyrosine residue and replacement of the 78th threonine residue of SEQ ID NO: 2 with an amino acid residue that does not form a hydrogen bond;
    5) replacement of the 46th proline residue of SEQ ID NO: 2 with a threonine, serine, or tyrosine residue and replacement of the 116th aspartic acid residue of SEQ ID NO: 2 with an amino acid that does not form a hydrogen bond; and
    6) replacement of the 46th proline residue of SEQ ID NO: 2 with a threonine, serine, or tyrosine residue, replacement of the 78th threonine residue of SEQ ID NO: 2 with an amino acid residue that does not form a hydrogen bond, and replacement of the 116th aspartic acid residue of SEQ ID NO: 2 with an amino acid that does not form a hydrogen bond.

    摘要翻译： 本发明提供一种修饰的生物素结合蛋白，其包含由SEQ ID NO：2或其修饰序列表示的氨基酸序列，并具有生物素结合活性和选自以下的置换：1）替换第36位丝氨酸残基 具有不形成氢键的氨基酸残基的SEQ ID NO：2; 2）用亲水氨基酸残基置换SEQ ID NO：2的第80个色氨酸残基; 3）用不形成氢键的氨基酸残基替换SEQ ID NO：2的第116位天冬氨酸残基; 4）用苏氨酸，丝氨酸或酪氨酸残基替换SEQ ID NO：2的第46个脯氨酸残基，并用不形成氢键的氨基酸残基替换SEQ ID NO：2的第78个苏氨酸残基; 5）用苏氨酸，丝氨酸或酪氨酸残基替换SEQ ID NO：2的第46个脯氨酸残基，用不形成氢键的氨基酸替换SEQ ID NO：2的第116位天冬氨酸残基; 和6）用苏氨酸，丝氨酸或酪氨酸残基替换SEQ ID NO：2的第46个脯氨酸残基，用不形成氢键的氨基酸残基替代SEQ ID NO：2的第78个苏氨酸残基， 并用不形成氢键的氨基酸替代SEQ ID NO：2的第116位天冬氨酸残基。