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    METHOD FOR PRODUCING ARTIFICIAL PIRNAS AND USES THEREOF FOR SILENCING GENE EXPRESSION
    4.
    发明公开
    METHOD FOR PRODUCING ARTIFICIAL PIRNAS AND USES THEREOF FOR SILENCING GENE EXPRESSION 审中-公开
    用于生产人工皮尔纳及其用途用于抑制基因表达

    公开(公告)号：EP3175705A1

    公开(公告)日：2017-06-07

    申请号：EP15197903.6

    申请日：2015-12-03

    申请人： European Molecular Biology Laboratory

    发明人： Pillai, Ramesh ,  Homolka, David ,  Pandey, Radha Raman

    IPC分类号： A01K67/00 ,  C12Q1/68 ,  C12N15/113 ,  C12N15/85

    CPC分类号： C12Q1/6806 ,  C12N15/113 ,  C12N15/8509 ,  C12N2310/10 ,  C12N2330/00 ,  C12N2330/31 ,  C12Q1/6883 ,  C12Q2600/178 ,  C12Q2521/313 ,  C12Q2525/207

    摘要： The present invention relates to a method for producing at least one artificial piRNA, comprising determining an endogenous piRNA population in a cell of an organism of interest; selecting a sub-population of said endogenous piRNA population based on binding of a MILI protein and/or an equivalent PIWI protein to said sub-population; identifying the sequence of at least one piRNA as selected, attaching said at least one piRNA sequence as identified 5' to the sequence of the transcript of a gene of interest, in order to generate at least one piRNA template, and slicing said piRNA template through binding of a MILI protein to said template and subsequent slicing of said bound piRNA template following a binding of MILI protein and/or MIWI2 protein in order to generate at least one artificial piRNA. The method can be used to produce pharmaceutical compositions comprising at least one artificial piRNA as produced according to the invention. Other aspects relates to a method for silencing the expression of a gene of interest in an organism of interest, comprising administering to said organism an effective amount of a pharmaceutical composition according to the invention, and a method for produing a non-genetically modified organism having an artificially silenced expression of at least one gene of interest based on the method of the invention.

    摘要翻译： 本发明涉及用于生产至少一种人造piRNA基因，包括内源性piRNA基因人口的确定性采矿在感兴趣的生物体的细胞的方法; 选择基于蛋白质MILI和/或等价的蛋白质到PIWI所述子种群的结合所述内源piRNA基因群的子群; 作为鉴定5“至感兴趣的基因的转录物的序列，以产生至少一个piRNA基因模板，和切片。所述piRNA基因模板通过标识至少一个piRNA基因的序列作为选择，连接所述至少一个piRNA基因序列 的蛋白质MILI所述模板和Said的后续切片的结合结合piRNA基因模板之后的结合MILI蛋白和/或蛋白MIWI2，以便产生至少一个人造piRNA基因。 该方法可用于生产药物组合物，包括至少一个人造piRNA基因作为产生雅丁于本发明。 其它方面涉及一种方法，用于在感兴趣的有机体中沉默中的感兴趣的基因的表达，包括给予所述生物体中有效量的雅丁发明的药物组合物，以及用于produing具有非遗传修饰的生物体的方法 人为地沉默基于本发明的方法的至少一个感兴趣的基因的表达。

    Aptamers for use against autoantibody-associated diseases
    6.
    发明公开
    Aptamers for use against autoantibody-associated diseases 审中-公开
    Aptamere zur Verwendung gegen Erkrankungen im Zusammenhang mitAutoantikörpern

    公开(公告)号：EP2982756A1

    公开(公告)日：2016-02-10

    申请号：EP14179715.9

    申请日：2014-08-04

    申请人： Berlin Cures Holding AG

    发明人： Müller, Johannes

    IPC分类号： C12N15/115 ,  G06F19/00 ,  A61K31/713

    CPC分类号： C12N15/115 ,  C12N2310/16 ,  C12N2330/00 ,  G01N33/6854 ,  G06F19/10 ,  Y02A90/26

    摘要： The present invention relates to new aptamer molecules for use in the treatment and/or diagnosis of autoimmune diseases associated with autoantibodies against G-protein coupled receptors, a pharmaceutical composition comprising such aptamer molecules, an apheresis column comprising such aptamer molecules and a method for the determination of nucleotide sequences for use as sequences of aptamer molecules.

    摘要翻译： 本发明涉及用于治疗和/或诊断与G蛋白偶联受体的自身抗体相关的自身免疫性疾病的新适体分子，包含这种适体分子的药物组合物，包含这种适体分子的单采血液柱以及用于 确定用作适体分子序列的核苷酸序列。

    APTAMERS FOR USE AGAINST AUTOANTIBODY-ASSOCIATED DISEASES
    8.
    发明公开
    APTAMERS FOR USE AGAINST AUTOANTIBODY-ASSOCIATED DISEASES 有权
    APTAMERS用于抗自体抗体相关的疾病

    公开(公告)号：EP3177723A1

    公开(公告)日：2017-06-14

    申请号：EP15744602.2

    申请日：2015-08-04

    申请人： Berlin Cures Holding AG

    发明人： MÜLLER, Johannes

    IPC分类号： C12N15/115 ,  G06F19/00 ,  A61K31/713

    CPC分类号： C12N15/115 ,  C12N2310/16 ,  C12N2330/00 ,  G01N33/6854 ,  G06F19/10 ,  Y02A90/26

    摘要： The present invention relates to new aptamer molecules for use in the treatment and/or diagnosis of autoimmune diseases associated with autoantibodies against G-protein coupled receptors, a pharmaceutical composition comprising such aptamer molecules, an apheresis column comprising such aptamer molecules and a method for the determination of nucleotide sequences for use as sequences of aptamer molecules.

    摘要翻译： 本发明涉及用于治疗和/或诊断与抗G蛋白偶联受体的自身抗体有关的自身免疫疾病的新型适体分子，包含此类适体分子的药物组合物，包含此类适体分子的apheresis柱和用于 确定用作适体分子序列的核苷酸序列。

    A method for production of single-stranded nucleic acids
    9.
    发明授权
    A method for production of single-stranded nucleic acids 有权
    恩法列宁

    公开(公告)号：EP2774996B1

    公开(公告)日：2016-08-10

    申请号：EP13001069.7

    申请日：2013-03-04

    申请人： Technische Universität Kaiserslautern

    发明人： Stadtmüller, Ralf, Dipl.-Biol. ,  Tippkötter, Nils, Dr.-Ing. ,  Ulber, Roland, Prof. Dr. rer. nat.

    IPC分类号： C12Q1/68

    CPC分类号： C12P19/34 ,  C12N15/115 ,  C12N2310/16 ,  C12N2330/00 ,  C12Q1/6844 ,  C12Q2525/131 ,  C12Q2525/191 ,  C12Q2525/205 ,  C12Q2531/125

    摘要： The invention relates to a method for production of single-stranded macronucleotides by amplifying and ligating an extended monomeric single-stranded target nucleic acid sequence (target ss ) into a repetitive cluster of double-stranded target nucleic acid sequences (target ds ), and subsequently cloning the construct into a vector (aptagene vector). The aptagene vector is transformed into host cells for replication of the aptagene and isolated in order to optain single-stranded target sequences (target ss ). The invention also relates to single-stranded nucleic acids, produced by a method of the invention.

    摘要翻译： 本发明涉及通过将扩展的单体单链靶核酸序列（靶标ss）扩增并连接到双链靶核酸序列（靶标ds）的重复簇中并随后将其连接到生产单链大分子核苷酸的方法 将构建体克隆到载体（aptagene载体）中。 将接头载体转化到宿主细胞中用于复制转基因并分离，以便选择单链靶序列（目标ss）。 本发明还涉及通过本发明的方法生产的单链核酸。