MALATE SYNTHASE REGULATORY SEQUENCES FOR HETEROLOGOUS GENE EXPRESSION IN PICHIA
    5.
    发明公开
    MALATE SYNTHASE REGULATORY SEQUENCES FOR HETEROLOGOUS GENE EXPRESSION IN PICHIA 审中-公开
    MALATSYNTHASEREGULATORSEQUENZEN FOR在毕赤异源基因表达

    公开(公告)号:EP1773861A2

    公开(公告)日:2007-04-18

    申请号:EP05805033.7

    申请日:2005-07-22

    申请人: GlycoFi, Inc.

    发明人: CHOI, Byung-Kwon

    摘要: The present invention relates to the isolation of DNA regulatory regions of the malate synthase 1 gene (MLS1) from the yeast, Pichia pastoris. The present invention further relates to the expression of heterologous proteins which have been introduced into a P. pastoris host cell and can be expressed under the regulation of the MLS1 5' regulatory promoter and MLS1 3'regulatory terminator regions. The MLS1 promoter is repressed in media containing glucose and derepressed under glucose starvation conditions or when acetate is present.