公开(公告)号：EP3099763A1

公开(公告)日：2016-12-07

申请号：EP15740946.7

申请日：2015-01-26

Applicant: William Marsh Rice University

Inventor： GONZALEZ, Ramon ,  CLOMBURG, James, M. ,  VICK, Jacob, E.

IPC: C10L1/06

CPC classification number: C12P7/64 ,  C10L1/02 ,  C10L1/04 ,  C12N9/00 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12Y101/011 ,  C12Y101/03013 ,  C12Y102/01 ,  C12Y102/01003 ,  C12Y103/01009 ,  C12Y114/00 ,  C12Y203/01009 ,  C12Y206/00 ,  C12Y401/99005 ,  C12Y402/01059 ,  Y02E50/343

Abstract: This disclosure describes enzymes from the type II (a discrete set of enzymes) fatty acid synthesis ("FAS") pathway that can be used in combination with thiolases to operate a functional reversal of the β-oxidation cycle. A combination of thiolases with one or more of 3-oxoacyl-[acyl-carrier-protein] reductase (FabG, others), 3-hydroxyacyl-[acp] dehydratase (FabA, FabZ, others), and enoyl-[acyl-carrier-protein] reductase (FabI, FabK, FabL, FabV, others) yields a functional reversal of the β-oxidation cycle. If only one or two enzymes are used, the remaining enzymes will be traditional beta oxidation enzymes. Once this cycle is coupled with the appropriate priming and termination pathways, the production of carboxylic acids, alcohols, hydrocarbons, amines and their α-, β-, and ω-functionalized derivatives from renewable carbon sources can be achieved.

Abstract translation: 本公开描述了可以与硫醇酶组合使用以操作β-氧化循环的功能性逆转的来自II型（一组离散酶）脂肪酸合成（“FAS”）途径的酶。 硫氧酶与一个或多个3-氧基酰基 - [酰基 - 载体 - 蛋白]还原酶（FabG，其他），3-羟基酰基 - [acp]脱水酶（FabA，FabZ等）和烯酰基 - - 蛋白]还原酶（FabI，FabK，FabL，FabV等）产生β-氧化循环的功能逆转。 如果只使用一种或两种酶，剩余的酶将是传统的β氧化酶。 一旦这个循环与适当的引发和终止途径相结合，就可以实现羧酸，醇，烃，胺及其可再生碳源的α-，β-和ω官能化衍生物的生产。

公开(公告)号：EP3008199A1

公开(公告)日：2016-04-20

申请号：EP14810750.1

申请日：2014-06-12

Applicant: Commonwealth Scientific and Industrial Research Organisation

Inventor： SCOTT, Colin ,  WILDING, Matthew ,  JERMIIN, Lars ,  PEAT, Thomas ,  NEWMAN, Janet

IPC: C12P7/44 ,  C07K14/21 ,  C12N9/10 ,  C12N15/54 ,  C12P13/00

CPC classification number: C12N9/1096 ,  C07K2299/00 ,  C12P7/64 ,  C12P13/00 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P13/04 ,  C12P17/10 ,  C12P21/02 ,  C12Y206/00 ,  C12Y206/01

Abstract: The present invention relates to pyruvate : ω-amino acid transaminases isolated from a
Pseudomonas species. The transaminases act on long chain amino acids and are capable of accepting substrates comprising 8 to 12 carbon atoms. The enzymes are suitable as biocatalysts for the manufacture of nylon.

公开(公告)号：EP3099763A4

公开(公告)日：2017-08-02

申请号：EP15740946

申请日：2015-01-26

Applicant: UNIV RICE WILLIAM M

Inventor： GONZALEZ RAMON ,  CLOMBURG JAMES M ,  VICK JACOB E

IPC: C10L1/06

CPC classification number: C12P7/64 ,  C10L1/02 ,  C10L1/04 ,  C12N9/00 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12Y101/011 ,  C12Y101/03013 ,  C12Y102/01 ,  C12Y102/01003 ,  C12Y103/01009 ,  C12Y114/00 ,  C12Y203/01009 ,  C12Y206/00 ,  C12Y401/99005 ,  C12Y402/01059 ,  Y02E50/343

Abstract: This disclosure describes enzymes from the type II (a discrete set of enzymes) fatty acid synthesis (“FAS”) pathway that can be used in combination with thiolases to operate a functional reversal of the β-oxidation cycle. A combination of thiolases with one or more of 3-oxoacyl-[acyl-carrier-protein] reductase (FabG, others), 3-hydroxyacyl-[acp] dehydratase (FabA, FabZ, others), and enoyl-[acyl-carrier-protein] reductase (FabI, FabK, FabL, FabV, others) yields a functional reversal of the β-oxidation cycle. If only one or two enzymes are used, the remaining enzymes will be traditional beta oxidation enzymes. Once this cycle is coupled with the appropriate priming and termination pathways, the production of carboxylic acids, alcohols, hydrocarbons, amines and their α-, β-, and ω-functionalized derivatives from renewable carbon sources can be achieved.

Abstract translation: 本公开描述了可以与硫解酶组合用于操作β-氧化循环的功能性反转的II型（一组离散的酶）脂肪酸合成（“FAS”）途径的酶。 硫解酶与一种或多种3-氧酰基 - [酰基 - 载体 - 蛋白]还原酶（FabG等），3-羟基酰基 - [acp]脱水酶（FabA，FabZ等）和烯酰基 - [酰基载体 蛋白]还原酶（FabI，FabK，FabL，FabV等）产生β-氧化循环的功能性反转。 如果只使用一种或两种酶，剩余的酶将是传统的β氧化酶。 一旦该循环与适当的引发和终止途径相结合，可以实现从可再生碳源生产羧酸，醇，烃，胺和它们的α-，β-和ω-官能化衍生物。

公开(公告)号：EP3008199A4

公开(公告)日：2017-01-18

申请号：EP14810750

申请日：2014-06-12

Applicant: COMMW SCIENT IND RES ORG

Inventor： SCOTT COLIN ,  WILDING MATTHEW ,  JERMIIN LARS ,  PEAT THOMAS ,  NEWMAN JANET

IPC: C12P7/44 ,  C07K14/21 ,  C12N9/10 ,  C12N15/54 ,  C12P7/64 ,  C12P13/00 ,  C12P13/02 ,  C12P13/04 ,  C12P17/10 ,  C12P21/02

CPC classification number: C12N9/1096 ,  C07K2299/00 ,  C12P7/64 ,  C12P13/00 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P13/04 ,  C12P17/10 ,  C12P21/02 ,  C12Y206/00 ,  C12Y206/01