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    BIOSYNTHESIS OF POLYKETIDES
    2.
    发明公开
    BIOSYNTHESIS OF POLYKETIDES 审中-公开

    公开(公告)号：EP3328991A2

    公开(公告)日：2018-06-06

    申请号：EP16831469.8

    申请日：2016-08-01

    Applicant: William Marsh Rice University

    Inventor： GONZALEZ, Ramon ,  CHEONG, Seokjung ,  CLOMBURG, James, M.

    IPC: C12N1/21 ,  C12N9/10 ,  C12N15/82

    CPC classification number: C12P7/26 ,  C12N9/1029

    Abstract: This disclosure generally relates to the use of microorganisms to make various functionalized polyketides through polyketoacyl-CoA thiolase-catalyzed non-decarboxylative condensation reactions instead of decarboxylative reactions catalyzed by polyketide synthases. Native or engineered polyketoacyl-CoA thiolases catalyze the non-decarboxylative Claisen condensation in an iterative manner (i.e. multiple rounds) between two either unsubstituted or functionalized ketoacyl-CoAs (and polyketoacyl-CoAs) serving as the primers and acyl- CoAs serving as the extender unit to generate (and elongate) polyketoacyl-CoAs. Before the next round of polyketoacyl-CoA thiolase reaction, the β-keto group of the polyketide chain of polyketoacyl-CoA can be reduced and modified step-wise by 3-OH-polyketoacyl-CoA dehydrogenase or polyketoenoyl-CoA hydratase or polyketoenoyl-CoA reductase. Dehydrogenase converts the β-keto group to β-hydroxy group. Hydratase converts the β- hydroxy group to α-β-double-bond. Reductase converts the α-β-double-bond to single bond. Spontaneous or thioesterase catalyzed termination reaction terminates the elongation of polyketide chain of polyketoacyl-CoA at any point through CoA removal and spontaneous reactions rearrange the structure, generating the final functional polyketide products.

    BIOSYNTHESIS OF PRODUCTS FROM 1-CARBON COMPOUNDS
    4.
    发明公开
    BIOSYNTHESIS OF PRODUCTS FROM 1-CARBON COMPOUNDS 审中-公开
    1-碳化合物产物的生物合成

    公开(公告)号：EP3212797A1

    公开(公告)日：2017-09-06

    申请号：EP15854300.9

    申请日：2015-10-29

    Applicant: William Marsh Rice University

    Inventor： GONZALEZ, Ramon ,  CHOU, Alexander ,  CLOMBURG, James

    IPC: C12P7/24 ,  C12P11/00 ,  C12N9/88

    CPC classification number: C12N9/88 ,  C12N15/52 ,  C12P1/04 ,  C12P5/02 ,  C12P7/04 ,  C12P7/24 ,  C12P7/40 ,  C12P11/00 ,  C12P13/001 ,  C12Y401/00 ,  Y02P20/142

    Abstract: An engineered microbe that contains a designed platform for the conversion of one-carbon substrates to chemical products is described. The designed platform embodies a new metabolic architecture that consolidates carbon fixation, central metabolism, and product synthesis into a single pathway. This is made possible by the key finding that 2-hydroxyacyl-CoA lyase, an enzyme in the α-oxidation pathway, is capable of catalyzing the C-C bond formation between formyl-CoA and aldehydes of different chain lengths, allowing for the elongation of the carbon backbone of said aldehyde by one-carbon units. These novel microbes present an opportunity for the production of chemicals from single-carbon feedstocks such as carbon dioxide, carbon monoxide, formate, formaldehyde, methanol or methane.

    Abstract translation: 描述了包含用于将单碳底物转化为化学产品的设计平台的工程微生物。 设计的平台体现了一种新的代谢结构，将碳固定，中心代谢和产物合成整合到单一途径中。 这可以通过以下关键发现实现：α-氧化途径中的酶2-羟基酰基-CoA裂解酶能够催化甲酰-CoA和不同链长度的醛之间的CC键形成，从而允许 所述醛的碳骨架以一个碳单位。 这些新型微生物为来自单一碳原料如二氧化碳，一氧化碳，甲酸盐，甲醛，甲醇或甲烷的化学品生产提供了机会。

    TYPE II FATTY ACID SYSTHESIS ENZYMES IN REVERSE B-OXIDATION
    10.
    发明公开
    TYPE II FATTY ACID SYSTHESIS ENZYMES IN REVERSE B-OXIDATION 审中-公开
    TYP-II-FETsÄYSYNTHESEENZYMEIN DER REVERSE-B-OXIDATION

    公开(公告)号：EP3099763A1

    公开(公告)日：2016-12-07

    申请号：EP15740946.7

    申请日：2015-01-26

    Applicant: William Marsh Rice University

    Inventor： GONZALEZ, Ramon ,  CLOMBURG, James, M. ,  VICK, Jacob, E.

    IPC: C10L1/06

    CPC classification number: C12P7/64 ,  C10L1/02 ,  C10L1/04 ,  C12N9/00 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12Y101/011 ,  C12Y101/03013 ,  C12Y102/01 ,  C12Y102/01003 ,  C12Y103/01009 ,  C12Y114/00 ,  C12Y203/01009 ,  C12Y206/00 ,  C12Y401/99005 ,  C12Y402/01059 ,  Y02E50/343

    Abstract: This disclosure describes enzymes from the type II (a discrete set of enzymes) fatty acid synthesis ("FAS") pathway that can be used in combination with thiolases to operate a functional reversal of the β-oxidation cycle. A combination of thiolases with one or more of 3-oxoacyl-[acyl-carrier-protein] reductase (FabG, others), 3-hydroxyacyl-[acp] dehydratase (FabA, FabZ, others), and enoyl-[acyl-carrier-protein] reductase (FabI, FabK, FabL, FabV, others) yields a functional reversal of the β-oxidation cycle. If only one or two enzymes are used, the remaining enzymes will be traditional beta oxidation enzymes. Once this cycle is coupled with the appropriate priming and termination pathways, the production of carboxylic acids, alcohols, hydrocarbons, amines and their α-, β-, and ω-functionalized derivatives from renewable carbon sources can be achieved.

    Abstract translation: 本公开描述了可以与硫醇酶组合使用以操作β-氧化循环的功能性逆转的来自II型（一组离散酶）脂肪酸合成（“FAS”）途径的酶。 硫氧酶与一个或多个3-氧基酰基 - [酰基 - 载体 - 蛋白]还原酶（FabG，其他），3-羟基酰基 - [acp]脱水酶（FabA，FabZ等）和烯酰基 - - 蛋白]还原酶（FabI，FabK，FabL，FabV等）产生β-氧化循环的功能逆转。 如果只使用一种或两种酶，剩余的酶将是传统的β氧化酶。 一旦这个循环与适当的引发和终止途径相结合，就可以实现羧酸，醇，烃，胺及其可再生碳源的α-，β-和ω官能化衍生物的生产。

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