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    Fluorometer
    4.
    发明公开
    Fluorometer 失效
    荧光计荧光计

    公开(公告)号：EP0207485A3

    公开(公告)日：1988-12-21

    申请号：EP86108894

    申请日：1986-06-30

    Applicant: DIATRON CORPORATION

    Inventor： Dandliker, Walter B. ,  Barr, Howard S. ,  Katzenstein, Henry S. ,  Watson, W. Keith R.

    IPC: G01N21/64 ,  H04N05/30 ,  G02F01/03 ,  G02B21/16

    CPC classification number: G01N21/6408 ,  G01N21/6456 ,  G01N21/6458 ,  G01N2201/1087 ,  G02B21/16

    Abstract: A fluorometer for measuring a particular fluorescence emanating from a specimen, including producing a burst of concentrated light energy and directing the concentrated light energy toward the specimen to produce a fluorescence from the specimen including the particular fluorescence. Preferably producing an image of the fluoresence. Detecting the fluorescence and producing a signal in accordance with the fluorescence. Controlling the passage of the image of the fluorescence for detecting within a particular time period so as to optimize the detection of the particular fluorescence. Timing the operation to sequence the detection of the fluorescence within the particular time period after the production of the burst of concentrated light energy. Scanning the fluorescence from the specimen for forming signals representative of the fluorescence from the specimen. Analyzing the signals to enhance the portion of the signal representing the particular fluorescence relative to the portion of the signal.

    A method for microphotometering microscope specimens, and apparatus for carrying out the method
    5.
    发明公开
    A method for microphotometering microscope specimens, and apparatus for carrying out the method 无效
    用于微观测定微量荧光标本的方法和实施方法的装置

    公开(公告)号：EP0155247A3

    公开(公告)日：1988-06-08

    申请号：EP85850055

    申请日：1985-02-19

    Applicant: SARASTRO AB

    Inventor： Carlsson, Kjell Sture

    IPC: G02B21/00

    CPC classification number: G02B21/002 ,  G01N21/474 ,  G01N21/5911 ,  G01N21/6456 ,  G01N2201/1087 ,  G02B21/0096

    Abstract: A method for microphotometering individual volume elements of a microscope specimen, comprising generating a luminous dot or cursor and progressively illuminating a plurality of part elements in the focal plane of the microscope (30) through the specimen. The mutual position between the specimen and the focal plane is then changed and a plurality of part elements in the focal plane are illuminated. Reflected and/or fluorescent light and transmitted light respectively created by the illumination is collected, detected and stored for generating a three-dimensional image of that part of the specimen composed of the volume elements. Illumination of multiples of part elements is deflected by deflecting the luminous cursor or by moving the specimen or by both deflecting the cursor and also displacing the specimen. The change in the relative mutual position between the specimen and the focal plane of the microscope (30) is effected either by displacing the specimen or the objective. Apparatus for carrying out the method include a specimen table (301), a microscope objective and light source (31, 32, 33). The table (301) or the objective are arranged for stepwise movement along the main axis of the microscope synchronously with punctilinear light scanning of the specimen. The table (301) is arranged for stepwise movement at right angles to the main axis and/or the light source (31, 32, 33) is arranged for deflection over the focal plane through the specimen.

    Method of and apparatus for real-time crystallographic axis orientation determination
    9.
    发明公开
    Method of and apparatus for real-time crystallographic axis orientation determination 失效
    实时晶体轴方位测定方法与装置

    公开(公告)号：EP0263621A3

    公开(公告)日：1990-01-10

    申请号：EP87308443.8

    申请日：1987-09-24

    Applicant: Weiser, Sidney

    Inventor： Weiser, Sidney

    IPC: G01N21/55

    CPC classification number: G01N21/55 ,  G01N2201/1045 ,  G01N2201/1087

    Abstract: A specific small area of a crystal sample (11) is scanned by a laser beam in a spiral pattern (2a, 2b). The laser beam is reflected different amounts for different beam positions to produce a reflectance pattern indicative of crystallographic orientation. The reflected beam radiation may be determined with a photodetector (12) and the reflectance pattern may be interpreted with circuitry (13) which also controls steering of the beam.

    Appareil et procédé de détection et de numération de particules fluorescentes, portées par un support solide
    10.
    发明公开
    Appareil et procédé de détection et de numération de particules fluorescentes, portées par un support solide 失效
    装置和方法，用于检测和荧光的计数，在固体载体颗粒承载。

    公开(公告)号：EP0333561A1

    公开(公告)日：1989-09-20

    申请号：EP89400656.8

    申请日：1989-03-08

    Applicant: CHEMUNEX

    Inventor： van Hoegaerden, Michel

    IPC: G01N21/64

    CPC classification number: G01N21/6456 ,  G01N15/1468 ,  G01N15/1475 ,  G01N21/6428 ,  G01N2021/0162 ,  G01N2201/1053 ,  G01N2201/1087

    Abstract: La présente invention est relative à un appa­reil de détection et de numération de particules fluores­centes ou rendues fluorescentes, portées par un support so­lide et à un procédé de détection desdites particules, à l'aide dudit appareil.
    L'appareil de détection et de numération de particules, présentes normalement, ou éventuellement conte­nues en tant que contaminants, dans un fluide liquide ou gazeux, ou dans un produit notamment alimentaire ou d'hygiène, par fluorimétrie, comprend une source lumineuse (10), des moyens de focalisation (12) du faisceau issu de ladite source lumineuse et au moins un moyen de détection (40) de la lumière fluorescente émise par les particules (60) présentes, et comprend en outre :
    - un support (50) approprié à la collecte des particules naturellement fluorescentes ou rendues fluo­rescentes à l'aide d'au moins un colorant approprié choisi dans le groupe qui comprend les colorants vitaux, les colo­rants de viabilité positive et des substances fluorescentes portées par des anticorps et/ou des sondes nucléiques,
    - des moyens de balayage (21, 31, 35) de la totalité de la surface du support à analyser, par ledit faisceau lumineux,
    - et, un microprocesseur (45) de traitement pourvu d'au moins un moyen d'enregistrement et de comptage simultané des signaux électriques transmis par le/les dispositifs de détection (40) et le système de balayage (21, 31, 35).

    Abstract translation: 设备，用于检测和计数颗粒，其荧光或荧光制成，由固体载体携带，并用于通过所述设备的方式检测所述颗粒的方法。 用于检测和计数粒子通常存在或可以包含在液体或气体流体中的污染物的形式的装置，或在尤其是产物的食品或卫生型产品，通过荧光，包括：光源（10） 装置，用于聚焦（12）从所述光源和用于检测至少一个装置（40）的荧光由存在的颗粒（60）所发射的，因此，一个梁包括：一个支撑件（50）适合于收集颗粒，其自然 荧光或通过从包括重要的污渍，以积极的活力和通过抗体和/或核酸探针携带荧光物质上的污渍的组中选择至少一种合适的染色剂的手段已经进行了荧光; 装置，用于扫描（21，31，35），所述支撑表面的整体由所述光束来分析; 并设置有至少一个微处理器（45）装置，用于记录和计数同时电信号反式由检测装置（一个或多个）（40）和扫描系统（21，31，35）mitted。

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