公开(公告)号：EP2401920B1

公开(公告)日：2013-02-20

申请号：EP11172382.1

申请日：2011-07-01

申请人： DR. SCHAER SPA

发明人： Cerne, Virna Lucia

IPC分类号： C07K14/425 ,  A21D13/06 ,  A21D2/26 ,  A23L1/16

CPC分类号： A21D13/066 ,  A21D2/265 ,  A23J1/12 ,  A23J1/14 ,  A23J3/14 ,  A23L7/109 ,  C07K14/415 ,  C07K14/425

公开(公告)号：EP2380986A1

公开(公告)日：2011-10-26

申请号：EP11163021.6

申请日：2007-01-04

申请人： BASF Plant Science GmbH

发明人： Frank, Markus ,  Schweizer, Patrick ,  Douchkov, Dimitar

IPC分类号： C12N15/82 ,  C07K14/425 ,  A01H5/00 ,  C07K14/415

CPC分类号： C07K14/415 ,  C12N15/8218 ,  C12N15/8282

摘要： The invention relates to a method of generating or increasing a pathogen resistance in plants by reducing the expression of at least one stomatin polypeptide or a functional equivalent thereof. The invention relates to novel nucleic acid sequences coding for a Hordeum vulgare stomatin (HvSTM1) polynucleotide and describes homologous sequences (STM1) thereof, and to their use in methods for obtaining a pathogen resistance in plants, and to nucleic acid constructs, expression cassettes and vectors which comprise these sequences and which are suitable for mediating a fungal resistance in plants. The invention furthermore relates to transgenic organisms, in particular plants, which are transformed with these expression cassettes or vectors, and to cultures, parts or transgenic propagation material derived therefrom.

摘要翻译： 本发明涉及通过减少至少一种口油素多肽或其功能等同物的表达来产生或增加植物中病原体抗性的方法。 本发明涉及编码大麦草（Hordonum vulgare stomatin）（HvSTM1）多核苷酸的新型核酸序列，并描述了它们的同源序列（STM1），以及它们在获得植物中病原体抗性的方法中的用途以及核酸构建体，表达盒和 包含这些序列并且适合介导植物真菌抗性的载体。 本发明还涉及用这些表达盒或载体转化的转基因生物，特别是植物，以及由其衍生的培养物，部分或转基因繁殖材料。

公开(公告)号：EP2080769A2

公开(公告)日：2009-07-22

申请号：EP09156897.2

申请日：2005-06-29

申请人： Metanomics GmbH

发明人： Plesch, Gunnar ,  Puzio, Piotr ,  Blau, Astrid ,  Looser, Ralf ,  Wendel, Birgit ,  Kamlage, Beate ,  Schmitz, Oliver

IPC分类号： C07K14/425 ,  C07K14/395 ,  C12P1/00 ,  C12P1/04

CPC分类号： C12P1/00 ,  C07K14/245 ,  C07K14/395 ,  C12N15/8243 ,  C12P13/06 ,  C12P13/08 ,  C12Q1/025 ,  C12Q1/68 ,  C12Q1/6813 ,  C12Y101/05002

摘要： The present invention relates to a process for the production leucine and/or isoleucine and/or valine in a microorganism, a plant cell, a plant, a plant tissue or in one or more parts thereof. The invention furthermore relates to nucleic acid molecules, polypeptides, nucleic acid constructs, vectors, antisense molecules, antibodies, host cells, plant tissue, propagation material, harvested material, plants, microorganisms as well as agricultural compositions and to their use.

摘要翻译： 本发明涉及在微生物，植物细胞，植物，植物组织或其一个或多个部分中生产亮氨酸和/或异亮氨酸和/或缬氨酸的方法。 本发明还涉及核酸分子，多肽，核酸构建体，载体，反义分子，抗体，宿主细胞，植物组织，繁殖材料，收获的材料，植物，微生物以及农业组合物及其用途。

公开(公告)号：EP1523558B1

公开(公告)日：2007-07-04

申请号：EP02807566.1

申请日：2002-08-05

申请人： Era Plantech S.L.

发明人： LUDEVID MUGICA, Maria, Dolores ,  TORRENT QUETGLAS, Margarita ,  LASSERRE-RAMASSAMY, Sabine

IPC分类号： C12N15/62 ,  C12N15/18 ,  C12N15/82 ,  C07K14/585 ,  C07K14/425 ,  A01H5/00 ,  A01H5/10

CPC分类号： C07K14/585 ,  C07K14/425 ,  C12N15/8221 ,  C12N15/8257

摘要： A nucleic acid molecule is disclosed as containing a first nucleic acid sequence comprising a nucleotide sequence that encodes γ-zein protein, or a fragment thereof capable of directing and retaining a protein towards the endoplasmic reticulum (ER) of a plant cell; a second nucleic acid sequence containing a nucleotide sequence that encodes an amino acid sequence that is specifically cleavable by enzymatic or chemical means; and a third nucleic acid sequence containing the nucleotide sequence that encodes a peptide or protein of interest. Methods of using this nucleic acid molecule for transforming host plant cells and producing the peptide or protein of interest are also disclosed.

公开(公告)号：EP1312611A4

公开(公告)日：2006-12-06

申请号：EP00977971

申请日：2000-11-28

申请人： NAT INST OF ADVANCED IND SCIEN

发明人： HATAKEDA KIYOTAKA ,  IKUSHIMA YUTAKA ,  MARUYAMA SUSUMU ,  TORII KAZUO

IPC分类号： C07C227/20 ,  C07K1/12 ,  A23J3/30 ,  A23L1/305 ,  A61K31/195 ,  C07C227/18 ,  C07C227/28 ,  C07C229/00 ,  C07C229/02 ,  C07C237/12 ,  C07C237/22 ,  C07K14/415 ,  C07K14/425 ,  G01N33/68

CPC分类号： C07K1/12 ,  A23J3/30 ,  A23L33/175 ,  A23L33/18 ,  C07C227/18 ,  C07C227/28 ,  Y02P20/544 ,  C07C229/02

摘要： A method of continuously and quickly degrading a protein into peptides and/or amino acids in supercritical water or high-pressure hot water at around the supercritical point. Namely, a method of continuously and quickly producing peptides and/or amino acids from a protein which comprises using a flow-through type reactor consisting of a substrate-introducing line for supplying a substrate, a pre-heating oven for supplying high-pressure hot water, a reaction oven for heating a liquid reaction mixture and reacting the same and a cooling unit for cooling the liquid reaction mixture, and continuously hydrolyzing the substrate protein supplied into the reaction oven in supercritical water or high-pressure hot water at around the supercritical point under a pressure of 22.05 MPa or above and at a temperature of 375?C or above; and a method of continuously and quickly hydrolyzing a protein by the above-described process. By using this method, it is possible to obtain preparations which are substantially different from the products obtained by the batch method and contaminated with little secondary and tertiary reaction products. Thus, this method is highly useful particularly in, for example, production of physiologically active peptides and pretreatment for the evaluation and analysis of amino acid composition.

公开(公告)号：EP1508805A1

公开(公告)日：2005-02-23

申请号：EP04019560.4

申请日：2004-08-18

申请人： Seikagaku Corporation

发明人： Ishimaru, Takeshi ,  Miyaura, Shuichi

IPC分类号： G01N33/50 ,  C12N9/96 ,  C07K14/415 ,  C07K14/425

CPC分类号： A61K39/395 ,  C12N9/96 ,  G01N33/5097 ,  G01N33/56961

摘要： The invention provides a novel stabilizing agent for enhancing stability of a specific-binding-pair-forming substance during storage and a novel blocking agent for preventing non-specific adsorption for use in an assay employing a specific-binding-pair-forming substance. Specifically, the invention provides a stabilizing agent for a specific-binding-pair-forming substance, the agent containing a plant-derived polypeptide as an active ingredient; a blocking agent for preventing non-specific adsorption in an assay employing a specific-binding-pair-forming substance; a composition for use in an assay employing a specific-binding-pair-forming substance, the composition containing the specific-binding-pair-forming substance and the stabilizing agent or the blocking agent; and a kit for use in an assay employing a specific-binding-pair-forming substance, the kit containing a stabilizing agent or a blocking agent for preventing non-specific adsorption.

摘要翻译： 本发明提供了一种用于增强贮藏期间特异性结合对形成物质的稳定性的新型稳定剂，以及用于防止非特异性吸附用于使用特异性结合对形成物质的检测中的新型阻断剂。 具体地，本发明提供了特异性结合对形成物质的稳定剂，所述物质含有植物来源的多肽作为活性成分; 用于在使用特异性结合对形成物质的测定中防止非特异性吸附的阻断剂; 用于使用特异性结合对形成物质的测定中使用的组合物，所述组合物含有特异性结合对形成物质和稳定剂或封闭剂; 以及用于使用特异性结合对形成物质的试验盒的试剂盒，该试剂盒含有稳定剂或阻止非特异性吸附的封闭剂。

公开(公告)号：EP1381625B1

公开(公告)日：2004-11-24

申请号：EP01972260.2

申请日：2001-09-28

申请人： Amersham Biosciences UK Limited

发明人： STUBBS, Simon L. J. Amersham Biosciences UK Ltd ,  JONES, Ann Elizabeth Amersham Biosciences UK Ltd. ,  MICHAEL, Nigel Paul Amersham Biosciences UK Ltd. ,  THOMAS, Nicholas Amersham Biosciences UK Ltd

IPC分类号： C07K14/425 ,  C12N15/00 ,  C12N15/62 ,  C12N15/10 ,  G01N33/00 ,  C12Q1/68

CPC分类号： C07K14/43595 ,  C07K2319/00

摘要： The present invention provides novel engineered derivatives of green fluorescent protein (GFP) which have an amino acid sequence which is modified by amino acid substitution compared with the amino acid sequence of wild type Green Fluorescent Protein. The modified GFPs exhibit enhanced fluorescence relative to wtGFP when expressed in non-homologous cells at temperatures above 30 DEG C, and when excited at about 490 nm compared to the parent proteins, i.e. wtGFP. An example of a preferred protein is F64L-S175G-E222G-GFP. The modified GFPs provide a means for detecting GFP reporters in mammalian cells at lower levels of expression and/or increased sensitivity relative to wtGFP. This greatly improves the usefulness of fluorescent proteins in studying cellular functions in living cells.

公开(公告)号：EP1319074A2

公开(公告)日：2003-06-18

申请号：EP01272802.8

申请日：2001-12-06

申请人： Post Genome Institute Co., Ltd

发明人： INOUE, Akio ,  SHIMIZU, Yoshihiro ,  UEDA, Takuya,4-204, Tokyo Univ. Syukusya

IPC分类号： C12N15/20 ,  C12N15/62 ,  C12P21/02 ,  C07K14/425 ,  C07K1/00 ,  C07K1/13 ,  C07K1/32

CPC分类号： C12P21/02

摘要： It is intended to construct a reaction system whereby a peptide produced in an in vitro peptide synthesis system can be efficiently isolated at a high purity from the reaction system, and, at the same time, to resolve the problem of the consumption of energy in the reaction system. A process for producing a peptide or a peptide derivative by using a reaction system of transcribing a DNA into an RNA and then translating the RNA produced or a reaction system of translating an RNA in vitro characterized in that a part or all of protein components constituting the transcription/ translation reaction system are labeled with one of a pair of substances adhering to each other and the other substance is used as an adsorbent for capturing said labeled protein components after translating.

公开(公告)号：EP4142523A1

公开(公告)日：2023-03-08

申请号：EP21731006.9

申请日：2021-04-29

申请人： Cargill, Incorporated

发明人： GAJADEERA, Chathurada Sugeeshvarie ,  PORTER, Michael A.

IPC分类号： A23L33/18 ,  A23J1/12 ,  A23J3/34 ,  C07K14/425

公开(公告)号：EP3775925A1

公开(公告)日：2021-02-17

申请号：EP19732392.6

申请日：2019-06-26

申请人： Unilever PLC ,  Unilever N.V.

发明人： AINGER, Nicholas, John ,  GALPIN, Annie, Jaye

IPC分类号： G01N33/68 ,  C07K14/425