EP0790991A4 -
    7.
    发明公开
    EP0790991A4 - 失效
    EP0790991A4 - Google专利

    公开(公告)号:EP0790991A4

    公开(公告)日:1997-09-03

    申请号:EP95940524

    申请日:1995-11-07

    摘要: A chemiluminescent assay method and compositions are described which use a haloalkoxy group-substituted dioxetane which is deprotected by a hydrolytic enzyme to undergo a chemiluminescent reaction. Chemiluminescent 1,2-dioxetane compounds substituted on the dioxetane ring with a haloalkoxy group which can be triggered by a reagent to generate light are disclosed. Haloalkoxy group-substituted dioxetanes are useful for the detection of triggering agents including enzymes. The enzyme may be present alone or linked to a member of a specific binding pair in an immunoassay, DNA probe assay or other assay where the enzyme is bound to a reporter molecule.

    摘要翻译: 描述了使用被水解酶脱保护的卤代烷氧基取代的二氧杂环丁烷进行化学发光反应的化学发光测定方法和组合物。 披露了在二恶丁环环上被卤代烷氧基取代的化学发光1,2-二氧杂环丁烷化合物,其可以被试剂触发以产生光。 卤代烷氧基取代的二氧杂环丁烷可用于检测引发剂,包括酶。 所述酶可以单独存在或在免疫测定,DNA探针测定或其他测定中与酶结合于报道分子的其他测定中连接至特异性结合对的成员。