利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

30 条记录 (耗时 0.06 秒)

    Methods for packaging oligonucleotides into virus-like particles of rna bacteriophages
    3.
    发明专利
    Methods for packaging oligonucleotides into virus-like particles of rna bacteriophages 有权
    将寡核苷酸包裹在病毒类病毒颗粒中的方法

    公开(公告)号：JP2014097057A

    公开(公告)日：2014-05-29

    申请号：JP2013255691

    申请日：2013-12-11

    申请人： Cytos Biotechnology Ag ,  サイトス バイオテクノロジー アーゲー

    发明人： MATTHIAS KINZLER ,  KARL PROBA

    IPC分类号： C12N7/00 ,  C12N15/09 ,  C12N15/117

    CPC分类号： C12N15/86 ,  A61K2039/5258 ,  C12N7/00 ,  C12N15/117 ,  C12N2310/17 ,  C12N2320/32 ,  C12N2795/00034 ,  C12N2795/18122 ,  C12N2795/18123

    摘要： PROBLEM TO BE SOLVED: To provide methods of "reassembling" virus-like particles packaged with oligonucleotides whose efficiency to generate packaging products with extremely high purity is significantly improved.SOLUTION: Provided are methods for producing compositions comprising (i) a virus-like particle that is a virus-like particle of an RNA bacteriophage, and (ii) an oligonucleotide that is packaged into the virus-like particle. Provided are methods for producing nucleotide compositions comprising oligonucleotides suitable to be used in the methods. Provided are nucleotide compositions obtainable by the methods and uses thereof. Provided are compositions comprising said (i) virus-like particle of an RNA bacteriophage and said (ii) oligonucleotide that is packaged into the virus-like particle, obtainable by the methods and having a purity of at least 98%, most preferably of at least 99%.

    摘要翻译： 要解决的问题：提供“重新组装”与寡核苷酸一起包装的病毒样颗粒的方法，其显着提高了产生具有极高纯度的包装产品的效率。解决方案：提供了用于生产组合物的方法，其包含（i）病毒样颗粒 即RNA噬菌体的病毒样颗粒，和（ii）包装在病毒样颗粒中的寡核苷酸。 提供了用于产生包含适合用于该方法的寡核苷酸的核苷酸组合物的方法。 提供的是通过其方法和用途可获得的核苷酸组合物。 提供了包含所述（i）RNA噬菌体的病毒样颗粒和所述（ii）包装在病毒样颗粒中的寡核苷酸的组合物，其可通过方法获得并且纯度为至少98％，最优选为 至少99％。