公开(公告)号：US20120077181A1

公开(公告)日：2012-03-29

申请号：US13201016

申请日：2010-02-18

Applicant: Timo Schmidt ,  Lothar Just ,  Franz Maser ,  Holger Becker

Inventor： Timo Schmidt ,  Lothar Just ,  Franz Maser ,  Holger Becker

IPC: C12N5/071 ,  C12N5/0775 ,  C12N5/076 ,  C12N5/0793 ,  C12N5/0781 ,  C12N5/0783 ,  C12N5/04 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N5/10 ,  C12N1/21 ,  C12N1/15 ,  C12N1/19 ,  C12Q1/02 ,  C12N5/0735

CPC classification number: A01N1/0231 ,  C12N5/0657 ,  C12N2533/54

Abstract: The cryopreservation of cells and tissue cultures for long term storage of cells, cell constructs or three-dimensional complex tissues assemblies is based on the use of a collagen cell carrier (CCC) having a specific composition and thickness and appropriate mechanical properties which are maintained after thawing. The collagen cell carrier provides a suitable support for the cryopreservation resulting in high survival rates after thawing and providing cells and tissue assemblies already adhered to a mechanically stable and biocompatible support. Frozen collagen carrier-cells assemblies, the frozen artificial cell constructs or three-dimensional complex tissue assemblies obtainable by the method and the use thereof after thawing are also disclosed.

Abstract translation: 细胞和组织培养物的冷冻保存用于细胞，细胞构建体或三维复合组织组装的长期储存是基于使用具有特定组成和厚度以及适当机械性质的胶原细胞载体（CCC），其保持在 解冻 胶原细胞载体为冷冻保存提供合适的支持，导致解冻后的高存活率，并提供已经粘附于机械稳定和生物相容性支持物的细胞和组织组件。 还公开了冷冻胶原载体细胞组合物，通过其解冻后的方法和其使用可获得的冷冻人造细胞构建体或三维复合组织组装体。

公开(公告)号：US20090023194A1

公开(公告)日：2009-01-22

申请号：US12138258

申请日：2008-06-12

Applicant: Timo Schmidt ,  Lothar Just

Inventor： Timo Schmidt ,  Lothar Just

IPC: C12N13/00 ,  C12M3/00

CPC classification number: C12M35/04 ,  C12M23/04 ,  C12M29/06 ,  C12M35/02

Abstract: The present invention relates to a device and a method for the cultivation and generation of biological material.

Abstract translation: 本发明涉及用于培养和产生生物材料的装置和方法。

公开(公告)号：US09267101B2

公开(公告)日：2016-02-23

申请号：US13878143

申请日：2010-10-08

Applicant: Timo Schmidt ,  Lothar Just ,  Holger Becker

Inventor： Timo Schmidt ,  Lothar Just ,  Holger Becker

IPC: C12M1/12 ,  C12M1/32 ,  C12M3/00 ,  C12M1/42

CPC classification number: C12M25/04 ,  C12M23/12 ,  C12M23/44 ,  C12M25/14 ,  C12M35/08

Abstract: A cell culture device allows co-cultivation on both sides of a membrane and has an outer tubular body with upper end having an opening and lower end closed by the membrane, an inner surface and an outer surface, which can be colonized with biological material, a complementary tubular body whose inner diameter fits onto the outer diameter of the lower end and a hanger component with upper end and a lower end. A flange extends from the upper end and the outer diameter component fits the inner diameter of the outer tubular body. The outer tubular body in a first step is connected to the complementary tubular body to form a standing insert for a first cell cultivation, and in a second step wherein the outer tubular body is disengaged from the complementary tubular body and is attached to the hanger component for a second cell cultivation.

Abstract translation: 细胞培养装置允许在膜的两侧共同培养，并且具有外管状体，其上端具有通过膜封闭的开口和下端，内表面和外表面，其可以被生物材料定殖， 其内径配合到下端的外径上的互补管状体和具有上端和下端的衣架部件。 凸缘从上端延伸并且外径部件配合外管体的内径。 第一步骤中的外部管状体连接到互补管状体以形成用于第一细胞培养的站立插入物，并且在第二步骤中，外部管状体与互补管状体脱离并附接到衣架部件 用于第二细胞培养。

公开(公告)号：US20100233140A1

公开(公告)日：2010-09-16

申请号：US12705682

申请日：2010-02-15

Applicant: LOTHAR JUST ,  TIMO SCHMIDT ,  FRANZ MASER

Inventor： LOTHAR JUST ,  TIMO SCHMIDT ,  FRANZ MASER

IPC: A61K35/12 ,  C12N5/07 ,  C12N5/0789 ,  A61P43/00

CPC classification number: A61K47/42 ,  C12N5/0068 ,  C12N2533/54

Abstract: The present invention relates to the use of a collagen-containing composition for the cultivation of biological cells, a method for the cultivation of biological cells, a method for the implantation of biological material into an organism and a method for the improvement of a composition in its suitability for the cultivation of biological cells.

Abstract translation: 本发明涉及用于培养生物细胞的含胶原蛋白的组合物的用途，培养生物细胞的方法，将生物材料植入生物体中的方法以及改进组合物的方法 其适用于培养生物细胞。

公开(公告)号：US20130267019A1

公开(公告)日：2013-10-10

申请号：US13878143

申请日：2010-10-08

Applicant: Timo Schmidt ,  Lothar Just ,  Holger Becker

Inventor： Timo Schmidt ,  Lothar Just ,  Holger Becker

IPC: C12M1/12

CPC classification number: C12M25/04 ,  C12M23/12 ,  C12M23/44 ,  C12M25/14 ,  C12M35/08

Abstract: A cell culture device allows co-cultivation on both sides of a membrane and has an outer tubular body with upper end having an opening and lower end closed by the membrane, an inner surface and an outer surface, which can be colonized with biological material, a complementary tubular body whose inner diameter fits onto the outer diameter of the lower end and a hanger component with upper end and a lower end. A flange extends from the upper end and the outer diameter component fits the inner diameter of the outer tubular body. The outer tubular body in a first step is connected to the complementary tubular body to form a standing insert for a first cell cultivation, and in a second step wherein the outer tubular body is disengaged from the complementary tubular body and is attached to the hanger component for a second cell cultivation.

Abstract translation: 细胞培养装置允许在膜的两侧共同培养，并且具有外管状体，其上端具有通过膜封闭的开口和下端，内表面和外表面，其可以被生物材料定殖， 其内径配合到下端的外径上的互补管状体和具有上端和下端的衣架部件。 凸缘从上端延伸并且外径部件配合外管体的内径。 第一步骤中的外部管状体连接到互补管状体以形成用于第一细胞培养的站立插入物，并且在第二步骤中，外部管状体与互补管状体脱离并附接到衣架部件 用于第二细胞培养。