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    COMPOSITION AND METHOD FOR EFFICIENT DELIVERY OF NUCLEIC ACIDS TO CELLS USING CHITOSAN
    1.
    发明申请
    COMPOSITION AND METHOD FOR EFFICIENT DELIVERY OF NUCLEIC ACIDS TO CELLS USING CHITOSAN 审中-公开
    使用CHITOSAN将核酸向细胞有效输送的组合物和方法

    公开(公告)号：US20090075383A1

    公开(公告)日：2009-03-19

    申请号：US12092498

    申请日：2006-11-06

    申请人： Michael D. Buschmann ,  Marc Lavertu ,  Stephane Methot

    发明人： Michael D. Buschmann ,  Marc Lavertu ,  Stephane Methot

    IPC分类号： C12N15/85

    CPC分类号： C12N15/87 ,  A61K31/70 ,  A61K31/715

    摘要： There is disclosed a composition and a method for the efficient non-viral delivery of nucleic acids to cells using chitosan. In order to achieve high efficiency of transfection, the composition contains a nucleic acid and a chitosan that has the following physico-chemical properties: a combination of a number-average molecular weight between 8 kDa and 185 kDa and a degree of deacetylation between 72% and 92%. The chitosan molecule can also present additional physiochemical properties such as a block distribution of acetyl groups obtained by a heterogeneous treatment of chitin, and/or a polydispersity index between 1.4 and 7.0. By correctly controlling these parameters, efficient delivery systems may be produced that are effective when optimized for different conditions such as the pH of transfection media and amine-to-phosphate ratio.

    摘要翻译： 公开了使用壳聚糖将核酸有效非病毒递送至细胞的组合物和方法。 为了实现高转染效率，组合物含有具有以下物理化学性质的核酸和壳聚糖：数均分子量在8kDa至185kDa之间，脱乙酰度为72％ 和92％。 壳聚糖分子还可以呈现另外的物理化学性质，例如通过几丁质的多相处理获得的乙酰基的嵌段分布和/或1.4-7.0之间的多分散性指数。 通过正确控制这些参数，可以产生有效的递送系统，其在针对不同条件如转染培养基的pH和胺与磷酸酯比例进行优化时是有效的。

    Universal mechanical testing device
    2.
    发明授权
    Universal mechanical testing device 失效
    通用机械试验装置

    公开(公告)号：US06332364B1

    公开(公告)日：2001-12-25

    申请号：US09553063

    申请日：2000-04-20

    申请人： Michael D. Buschmann ,  Martin Garon ,  Matthieu Ouellet ,  Marc Lavertu

    发明人： Michael D. Buschmann ,  Martin Garon ,  Matthieu Ouellet ,  Marc Lavertu

    IPC分类号： G01N300

    CPC分类号： G01N3/08 ,  G01N3/20 ,  G01N33/15 ,  G01N2203/0071

    摘要： A universal material testing device which includes a frame; an actuator mounted on the frame for controlling a displacement of a sample to be tested; a load cell movably mounted on the frame and adapted to abut against the sample for detecting a force applied thereon by the actuator, the load cell producing a signal corresponding to the force detected; a signal conditioning unit for reducing input noise and for processing of the signal and executing specific tests by coordination of displacement control and load signals received for processing from the load cell; and a detachable chamber with humidifying media for humidification of the sample environment confined within the chamber and separated frm the sample so as to avoid potential damaging effects of humidity on the sample or on the device. The device is useful for determining certain material properties of a sample.

    摘要翻译： 一种通用材料测试装置，包括一个框架; 安装在框架上的致动器，用于控制待测样品的位移; 可移动地安装在所述框架上并且适于抵靠所述样本以用于检测由所述致动器施加在其上的力的测力传感器，所述测力传感器产生对应于所检测的力的信号; 信号调节单元，用于减少输入噪声和处理信号，并通过协调位移控制和从负载传感器处理接收的负载信号执行特定测试; 以及具有加湿介质的可拆卸室，用于加湿限定在室内的样品环境并分离出样品，以避免湿气对样品或装置的潜在的破坏性影响。 该装置对于确定样品的某些材料性质是有用的。

    GEL FORMATION OF POLYELECTROLYTE AQUEOUS SOLUTIONS BY THERMALLY INDUCED CHANGES IN IONIZATION STATE
    3.
    发明申请
    GEL FORMATION OF POLYELECTROLYTE AQUEOUS SOLUTIONS BY THERMALLY INDUCED CHANGES IN IONIZATION STATE 审中-公开
    通过热离子化引起的热变化，凝胶形成聚电解质水溶液

    公开(公告)号：US20110313056A1

    公开(公告)日：2011-12-22

    申请号：US13184953

    申请日：2011-07-18

    申请人： Michael D. Buschmann ,  Dominic Filion ,  Marc Lavertu

    发明人： Michael D. Buschmann ,  Dominic Filion ,  Marc Lavertu

    IPC分类号： A61K47/36

    CPC分类号： C08L5/08 ,  A61K8/19 ,  A61K8/736 ,  A61K41/0028 ,  A61K47/61 ,  A61K47/6903 ,  A61Q19/00 ,  C08B37/003

    摘要： There is disclosed aqueous solutions of polyelectrolytes that can be neutralized by increasing temperature, under suitable polyelectrolyte charge state conditions, in order to obtain a homogeneous gel. This can be achieved by adding an appropriate weak electrolyte to the polyelectrolyte so that an increase of temperature will lead to a spatially homogeneous neutralization of the polyelectrolyte via proton transfer between the polyelectrolyte and the weak electrolyte. The ability of such a system to be thermally sensitive and to gel upon heating relies on the temperature dependence of the ionization equilibrium for the two components. This thermally induced neutralization reduces electrostatic repulsion between polyelectrolyte molecules allowing the manifestation of attractive polyelectrolyte-polyelectrolyte hydrophobic interactions and hydrogen bonding that result in gel formation. These new kinds of thermo sensitive gels can be used for biomedical applications.

    摘要翻译： 公开了聚合电解质的水溶液，其可以在合适的聚电解质电荷状态条件下通过升高的温度中和，以获得均匀的凝胶。 这可以通过向聚电解质中添加适当的弱电解质来实现，使得温度的升高将导致聚电解质通过聚电解质和弱电解质之间的质子转移而空间均匀地中和。 这种系统在加热时热敏和凝胶的能力取决于两种组分的电离平衡的温度依赖性。 这种热诱导的中和降低了聚电解质分子之间的静电斥力，从而表现出导致凝胶形成的有吸引力的聚电解质 - 聚电解质疏水相互作用和氢键。 这些新型热敏凝胶可用于生物医学应用。

    GEL FORMATION OF POLYELECTROLYTE AQUEOUS SOLUTIONS BY THERMALLY INDUCED CHANGES IN IONIZATION STATE
    4.
    发明申请
    GEL FORMATION OF POLYELECTROLYTE AQUEOUS SOLUTIONS BY THERMALLY INDUCED CHANGES IN IONIZATION STATE 审中-公开
    通过热离子化引起的热变化，凝胶形成聚电解质水溶液

    公开(公告)号：US20090149421A1

    公开(公告)日：2009-06-11

    申请号：US12092484

    申请日：2006-11-06

    申请人： Michael D. Buschmann ,  Dominic Filion ,  Marc Lavertu

    发明人： Michael D. Buschmann ,  Dominic Filion ,  Marc Lavertu

    IPC分类号： A61K31/722

    CPC分类号： C08L5/08 ,  A61K8/19 ,  A61K8/736 ,  A61K41/0028 ,  A61K47/61 ,  A61K47/6903 ,  A61Q19/00 ,  C08B37/003

    摘要： There is disclosed aqueous solutions of polyelectrolytes that can be neutralized by increasing temperature, under suitable polyelectrolyte charge state conditions, in order to obtain a homogeneous gel. This can be achieved by adding an appropriate weak electrolyte to the polyelectrolyte so that an increase of temperature will lead to a spatially homogeneous neutralization of the polyelectrolyte via proton transfer between the polyelectrolyte and the weak electrolyte. The ability of such a system to be thermally sensitive and to gel upon heating relies on the temperature dependence of the ionization equilibrium for the two components. This thermally induced neutralization reduces electrostatic repulsion between polyelectrolyte molecules allowing the manifestation of attractive polyelectrolyte-polyelectrolyte hydrophobic interactions and hydrogen bonding that result in gel formation. These new kinds of thermo sensitive gels can be used for biomedical applications.

    摘要翻译： 公开了聚合电解质的水溶液，其可以在合适的聚电解质电荷状态条件下通过升高的温度中和，以获得均匀的凝胶。 这可以通过向聚电解质中添加适当的弱电解质来实现，使得温度的升高将导致聚电解质通过聚电解质和弱电解质之间的质子转移而空间均匀地中和。 这种系统在加热时热敏和凝胶的能力取决于两种组分的电离平衡的温度依赖性。 这种热诱导的中和降低了聚电解质分子之间的静电斥力，从而表现出导致凝胶形成的有吸引力的聚电解质 - 聚电解质疏水相互作用和氢键。 这些新型热敏凝胶可用于生物医学应用。

    GENE THERAPY FOR DIABETES WITH CHITOSAN-DELIVERED PLASMID ENCODING GLUCAGON-LIKE PEPTIDE 1
    7.
    发明申请
    GENE THERAPY FOR DIABETES WITH CHITOSAN-DELIVERED PLASMID ENCODING GLUCAGON-LIKE PEPTIDE 1 审中-公开
    基因治疗糖尿病患者用CHITOSAN提供的PLASMID编码葡萄糖样肽1

    公开(公告)号：US20130210717A1

    公开(公告)日：2013-08-15

    申请号：US13697082

    申请日：2011-05-10

    申请人： Abderrazzak Merzouki ,  Michael D. Buschmann

    发明人： Abderrazzak Merzouki ,  Michael D. Buschmann

    IPC分类号： A61K48/00 ,  A61K31/713 ,  A61K38/28

    CPC分类号： A61K48/005 ,  A61K9/0019 ,  A61K9/5161 ,  A61K31/713 ,  A61K38/00 ,  A61K38/28 ,  A61K48/0041 ,  C12N15/87

    摘要： Chitosan delivers a plasmid encoding Glucagon-Like Peptide 1 (GLP-1) to cells in a patient for gene therapy of diabetes. Chitosan is optimized for plasmid transfection by modulating three of its physico-chemical properties: degree of deacetylation (DDA), molecular weight (MW), and ratio of amines on chitosan to phosphates on DNA (N:P ratio), Chitosan 92-10-5 (DDA-MW-N:P) is more efficient than chitosans 80-10-10 and 80-80-5 in delivering a plasmid encoding luciferase or GLP-1(7-37) to cells. In the Zucker Diabetic Fatty (ZDF) rat model of diabetes, chitosan-delivered pVax plasmid encoding GLP-1 lowers glucose levels, increases insulin production and reduces weight gain.

    摘要翻译： 壳聚糖将编码胰高血糖素样肽1（GLP-1）的质粒递送到患者中用于糖尿病基因治疗的细胞中。 壳聚糖通过调节三种物理化学性质：脱乙酰度（DDA），分子量（MW）以及胺对壳聚糖与磷酸盐在DNA上的比例（N:P比），壳聚糖92-10 -5（DDA-MW-N：P）在将编码荧光素酶或GLP-1（7-37）的质粒递送至细胞时，比壳多糖80-10-10和80-80-5更有效。 在Zucker糖尿病脂肪（ZDF）大鼠糖尿病模型中，编码GLP-1的壳聚糖递送的pVax质粒降低葡萄糖水平，增加胰岛素产生并减轻体重增加。

    DRILL BURR AND METHOD FOR PERFORMING HOLES IN SUBCHONDRAL BONE TO PROMOTE CARTILAGE REPAIR
    10.
    发明申请
    DRILL BURR AND METHOD FOR PERFORMING HOLES IN SUBCHONDRAL BONE TO PROMOTE CARTILAGE REPAIR 审中-公开
    用于在骨髓中执行牙齿以促进修复的钻头和方法

    公开(公告)号：US20110034930A1

    公开(公告)日：2011-02-10

    申请号：US12919889

    申请日：2009-02-27

    申请人： Michael D. Buschmann ,  Hongmei Chen

    发明人： Michael D. Buschmann ,  Hongmei Chen

    IPC分类号： A61B17/16

    CPC分类号： A61B17/1615 ,  A61B2090/036

    摘要： A method for performing holes in subchondral bone to promote cartilage repair comprises selecting a drill burr having a drilling head and an axial stop, as a function of the distance between the tip of the drilling head and the axial stop and of a desired depth to reach a desired subchondral bone marrow compartment of a patient; drilling a hole through a base of a cartilage lesion with the drill burr to reach the desired subchondral bone marrow compartment of the patient; abutting the base of the cartilage lesion defining a periphery of the hole with the axial stop while drilling; and withdrawing the drill burr from the hole; whereby the hole has the desired depth and reaches the desired subchondral bone marrow compartment to promote cartilage repair.

    摘要翻译： 用于在软骨下骨中执行孔以促进软骨修复的方法包括选择具有钻头和轴向止动件的钻孔毛刺作为钻头头部与轴向止挡件之间的距离以及达到期望深度的函数 患者的期望的软骨下骨髓腔室; 用钻头钻孔穿过软骨损伤的基部，到达病人所需的软骨下骨髓腔; 在钻孔的同时，用轴向止动件邻接软骨损伤的基部来限定孔的周边; 并将钻头从孔中取出; 由此孔具有期望的深度并到达所需的软骨下骨髓间隔区以促进软骨修复。