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公开(公告)号：US09469848B2
公开(公告)日：2016-10-18
申请号：US13806398
申请日：2011-06-30
申请人： Peter Edvard Degn , Richard R. Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle , Elin Petersen
发明人： Peter Edvard Degn , Richard R. Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle , Elin Petersen
IPC分类号： C12P19/20 , C12N9/34 , C07H21/04 , C07K1/00 , C12N9/26 , A23L1/09 , C12C5/00 , C12C7/04 , C12P19/02 , C13K1/06
CPC分类号： C12N9/2408 , A23L29/35 , C12C5/004 , C12C7/04 , C12N9/2428 , C12P19/02 , C12P19/20 , C12Y302/01003 , C13K1/06 , Y02P20/52
摘要： The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly, the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
摘要翻译： 本发明涉及母体葡糖淀粉酶的组合变体，其在淀粉水解期间具有降低缩合产物合成的性质。 因此，母体葡糖淀粉酶的变体是适合的，例如用于酿造和葡萄糖浆生产中。 还公开了编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。
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公开(公告)号：US20130309726A9
公开(公告)日：2013-11-21
申请号：US13806398
申请日：2011-06-30
申请人： Peter Edvard Degn , Richard R. Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle , Elin Petersen
发明人： Peter Edvard Degn , Richard R. Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle , Elin Petersen
CPC分类号： C12N9/2408 , A23L29/35 , C12C5/004 , C12C7/04 , C12N9/2428 , C12P19/02 , C12P19/20 , C12Y302/01003 , C13K1/06 , Y02P20/52
摘要： The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
摘要翻译： 本发明涉及母体葡糖淀粉酶的组合变体，其在淀粉水解期间具有降低缩合产物合成的性质。 因此，母体葡糖淀粉酶的变体是适合的，例如用于酿造和葡萄糖浆生产中。 还公开了编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。
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公开(公告)号：US20120149070A1
公开(公告)日：2012-06-14
申请号：US13390769
申请日：2010-08-18
申请人： Peter Edvard Degn , Richard Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle
发明人： Peter Edvard Degn , Richard Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle
CPC分类号： C12N9/2408 , A23L29/35 , C12C5/004 , C12C7/04 , C12N9/2428 , C12P19/02 , C12P19/20 , C12Y302/01003 , C13K1/06 , Y02P20/52
摘要： The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
摘要翻译： 本发明涉及母体葡糖淀粉酶的组合变体，其在淀粉水解期间具有降低缩合产物合成的性质。 因此，母体葡糖淀粉酶的变体是适合的，例如用于酿造和葡萄糖浆生产中。 还公开了编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。
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公开(公告)号：US08809023B2
公开(公告)日：2014-08-19
申请号：US13390769
申请日：2010-08-18
申请人： Peter Edvard Degn , Richard Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle
发明人： Peter Edvard Degn , Richard Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle
CPC分类号： C12N9/2408 , A23L29/35 , C12C5/004 , C12C7/04 , C12N9/2428 , C12P19/02 , C12P19/20 , C12Y302/01003 , C13K1/06 , Y02P20/52
摘要： The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
摘要翻译： 本发明涉及母体葡糖淀粉酶的组合变体，其在淀粉水解期间具有降低缩合产物合成的性质。 因此，母体葡糖淀粉酶的变体是适合的，例如用于酿造和葡萄糖浆生产中。 还公开了编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。
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公开(公告)号：US20130102035A1
公开(公告)日：2013-04-25
申请号：US13806398
申请日：2011-06-30
申请人： Peter Edvard Degn , Richard R. Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle , Elin Petersen
发明人： Peter Edvard Degn , Richard R. Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle , Elin Petersen
CPC分类号： C12N9/2408 , A23L29/35 , C12C5/004 , C12C7/04 , C12N9/2428 , C12P19/02 , C12P19/20 , C12Y302/01003 , C13K1/06 , Y02P20/52
摘要： The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
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6.发明申请ENZYMATIC TREATMENT OF A PROTEINACEOUS SUBSTRATE BY ENZYMATIC REMOVAL OF FREE THIOLS 审中-公开通过酶联免疫测定法对蛋白质底物进行酶处理公开(公告)号：US20110123676A1
公开(公告)日：2011-05-26
申请号：US12875206
申请日：2010-09-03
CPC分类号： C12Y110/03002 , A23B4/22 , A23J3/04 , A23L13/74 , C12Y108/03002 , C12Y108/03003 , C12Y110/03001
摘要： The invention relates to the enzymatic treatment of a proteinaceous substrate with a first enzyme, such as a sulfhydryl oxidase. The first enzyme removes enzyme inhibitors, such as free thiols, present in the proteinaceous substrate. The removal of the inhibitory compounds in the substrate allows for an effective enzymatic action of a second enzyme such as protein cross-linking of the protein present in the substrate by tyrosinase enzymes.
摘要翻译： 本发明涉及用第一种酶如巯基氧化酶酶处理蛋白质底物。 第一种酶除去蛋白质底物中存在的酶抑制剂，如游离硫醇。 在底物中去除抑制性化合物允许第二酶的有效酶作用，例如酪氨酸酶在底物中存在的蛋白质的蛋白质交联。
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