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公开(公告)号：US09469848B2
公开(公告)日：2016-10-18
申请号：US13806398
申请日：2011-06-30
申请人： Peter Edvard Degn , Richard R. Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle , Elin Petersen
发明人： Peter Edvard Degn , Richard R. Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle , Elin Petersen
IPC分类号： C12P19/20 , C12N9/34 , C07H21/04 , C07K1/00 , C12N9/26 , A23L1/09 , C12C5/00 , C12C7/04 , C12P19/02 , C13K1/06
CPC分类号： C12N9/2408 , A23L29/35 , C12C5/004 , C12C7/04 , C12N9/2428 , C12P19/02 , C12P19/20 , C12Y302/01003 , C13K1/06 , Y02P20/52
摘要： The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly, the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
摘要翻译： 本发明涉及母体葡糖淀粉酶的组合变体，其在淀粉水解期间具有降低缩合产物合成的性质。 因此，母体葡糖淀粉酶的变体是适合的，例如用于酿造和葡萄糖浆生产中。 还公开了编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。
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公开(公告)号：US20130102035A1
公开(公告)日：2013-04-25
申请号：US13806398
申请日：2011-06-30
申请人： Peter Edvard Degn , Richard R. Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle , Elin Petersen
发明人： Peter Edvard Degn , Richard R. Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle , Elin Petersen
CPC分类号： C12N9/2408 , A23L29/35 , C12C5/004 , C12C7/04 , C12N9/2428 , C12P19/02 , C12P19/20 , C12Y302/01003 , C13K1/06 , Y02P20/52
摘要： The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
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公开(公告)号：US20130309726A9
公开(公告)日：2013-11-21
申请号：US13806398
申请日：2011-06-30
申请人： Peter Edvard Degn , Richard R. Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle , Elin Petersen
发明人： Peter Edvard Degn , Richard R. Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle , Elin Petersen
CPC分类号： C12N9/2408 , A23L29/35 , C12C5/004 , C12C7/04 , C12N9/2428 , C12P19/02 , C12P19/20 , C12Y302/01003 , C13K1/06 , Y02P20/52
摘要： The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
摘要翻译： 本发明涉及母体葡糖淀粉酶的组合变体，其在淀粉水解期间具有降低缩合产物合成的性质。 因此，母体葡糖淀粉酶的变体是适合的，例如用于酿造和葡萄糖浆生产中。 还公开了编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。
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4.发明申请COMBINATORIAL VARIANTS OF GLUCOAMYLASE WITH IMPROVED SPECIFIC ACTIVITY AND/OR THERMOSTABILITY 审中-公开具有改善的特异活性和/或热稳定性的葡萄糖酸酶的组合变体公开(公告)号：US20120164695A1
公开(公告)日：2012-06-28
申请号：US13390381
申请日：2010-08-18
IPC分类号： C12N9/34 , C12N15/63 , C12P19/14 , C12N5/10 , C12N1/15 , C12N1/21 , C12N1/19 , C12N15/56 , C12P7/02
CPC分类号： C12P19/14 , A23L29/35 , C12N9/2428 , C12P7/06 , C12Y302/01003 , C13K1/06 , Y02E50/17
摘要： Presently provided are variant glucoamylases displaying altered properties, such as improved thermostability and/or specific activity. Also disclosed are DNA sequences coding for the variants, vectors and host cells incorporating the DNA sequence, enzyme compositions, and methods of using the variants in various applications.
摘要翻译： 目前提供的是显示改变的性质的变体葡糖淀粉酶，例如改善的热稳定性和/或比活性。 还公开了编码掺入DNA序列的变体，载体和宿主细胞的DNA序列，酶组合物和在各种应用中使用变体的方法。
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公开(公告)号：US20120149087A1
公开(公告)日：2012-06-14
申请号：US13251116
申请日：2011-09-30
CPC分类号： C12N9/2428 , C12P7/06 , C12P19/02 , C12P19/14 , Y02E50/17
摘要： The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
摘要翻译： 本公开涉及具有改变的性质（例如改善的热稳定性和/或比活性）的母体葡糖淀粉酶的变体。 特别地，本公开提供了包含变体葡糖淀粉酶的组合物，包括淀粉水解组合物和清洁组合物。 本公开还涉及编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。
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公开(公告)号：US20100267114A1
公开(公告)日：2010-10-21
申请号：US12443255
申请日：2007-10-09
CPC分类号： C12N9/2428 , C12N15/09 , C12Y302/01003 , Y02E50/17
摘要： The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
摘要翻译： 本发明涉及具有改变的性质（例如改善的热稳定性和/或比活性）的母体葡糖淀粉酶的变体。 特别地，本发明提供包含变体葡糖淀粉酶，包括淀粉水解组合物，动物饲料组合物和清洁组合物的组合物。 本发明还涉及编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。
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公开(公告)号：US08592194B2
公开(公告)日：2013-11-26
申请号：US12681612
申请日：2008-04-08
申请人： Wolfgang Aehle , Richard R. Bott , Igor Nikolaev , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
发明人： Wolfgang Aehle , Richard R. Bott , Igor Nikolaev , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
CPC分类号： C12N5/10
摘要： The present invention relates to variant glucoamylases wherein the variant has altered properties (e.g., improved thermostability and/or specific activity) compared to a corresponding parent glucoamylase. The present invention also relates to enzyme compositions comprising a variant glucoamylase (e.g., starch hydrolyzing compositions); DNA constructs comprising polynucleotides encoding the variants and methods of producing the variant glucoamylases in host cells.
摘要翻译： 本发明涉及变体葡糖淀粉酶，其中与相应的母体葡糖淀粉酶相比，变体具有改变的性质（例如改善的热稳定性和/或比活性）。 本发明还涉及包含变体葡糖淀粉酶（例如淀粉水解组合物）的酶组合物; 包含编码变体的多核苷酸的DNA构建体和在宿主细胞中产生变体葡糖淀粉酶的方法。
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公开(公告)号：US20110020899A1
公开(公告)日：2011-01-27
申请号：US12681612
申请日：2008-04-08
申请人： Wolfgang Aehle , Richard R. Bott , Igor Nikolaev , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
发明人： Wolfgang Aehle , Richard R. Bott , Igor Nikolaev , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
CPC分类号： C12N5/10
摘要： The present invention relates to variant glucoamylases wherein the variant has altered properties (e.g., improved thermostability and/or specific activity) compared to a corresponding parent glucoamylase. The present invention also relates to enzyme compositions comprising a variant glucoamylase (e.g., starch hydrolyzing compositions); DNA constructs comprising polynucleotides encoding the variants and methods of producing the variant glucoamylases in host cells
摘要翻译： 本发明涉及变体葡糖淀粉酶，其中与相应的母体葡糖淀粉酶相比，变体具有改变的性质（例如改善的热稳定性和/或比活性）。 本发明还涉及包含变体葡糖淀粉酶（例如淀粉水解组合物）的酶组合物; 包含编码变体的多核苷酸的DNA构建体和在宿主细胞中产生变体葡糖淀粉酶的方法
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公开(公告)号：US20090275080A1
公开(公告)日：2009-11-05
申请号：US12292563
申请日：2008-11-20
CPC分类号： C12N9/2428 , C12P7/06 , C12P19/02 , C12P19/14 , Y02E50/17
摘要： The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
摘要翻译： 本公开涉及具有改变的性质（例如改善的热稳定性和/或比活性）的母体葡糖淀粉酶的变体。 特别地，本公开提供了包含变体葡糖淀粉酶的组合物，包括淀粉水解组合物和清洁组合物。 本公开还涉及编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。
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公开(公告)号：US09447397B2
公开(公告)日：2016-09-20
申请号：US12443255
申请日：2007-10-09
CPC分类号： C12N9/2428 , C12N15/09 , C12Y302/01003 , Y02E50/17
摘要： The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
摘要翻译： 本发明涉及具有改变的性质（例如改善的热稳定性和/或比活性）的母体葡糖淀粉酶的变体。 特别地，本发明提供包含变体葡糖淀粉酶，包括淀粉水解组合物，动物饲料组合物和清洁组合物的组合物。 本发明还涉及编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。
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