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公开(公告)号：US20140051106A1
公开(公告)日：2014-02-20
申请号：US13985243
申请日：2012-02-10
申请人： Christopher Joseph Schofield , Alexander Wolf , Wei Ge , Armin Thalhammer , Christoph Loenarz , Peter John Ratcliffe , Matthew Edward Cockman
发明人： Christopher Joseph Schofield , Alexander Wolf , Wei Ge , Armin Thalhammer , Christoph Loenarz , Peter John Ratcliffe , Matthew Edward Cockman
IPC分类号： C12Q1/26
CPC分类号： C12Q1/26 , G01N2333/90245
摘要： The present invention relates to assays for monitoring activity of OGFOD1 activity, in particular, to assays for identifying modulators of OGFOD1 activity. The invention also relates to assays to monitor the prolyl hydroxylase activity of OGFOD1 on its substrate, the human ribosomal protein RPS23. The invention also enables the introduction of 3-hydroxyprolyl residues into peptides and proteins.
摘要翻译： 本发明涉及用于监测OGFOD1活性的活性的测定法，特别是用于鉴定OGFOD1活性调节剂的测定法。 本发明还涉及监测OGFOD1在其底物，人核糖体蛋白RPS23上的脯氨酰羟化酶活性的测定。 本发明还能够将3-羟基脯氨酸残基引入肽和蛋白质。
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2.发明授权公开(公告)号：US07778779B2
公开(公告)日：2010-08-17
申请号：US10531662
申请日：2003-10-16
申请人： Peter John Ratcliffe , Christopher William Pugh , Christopher Joseph Schofield , Kirsty Sarah Hewitson , Jonathan Mark Elkins
发明人： Peter John Ratcliffe , Christopher William Pugh , Christopher Joseph Schofield , Kirsty Sarah Hewitson , Jonathan Mark Elkins
CPC分类号： A61K31/16 , A61K31/395 , G06F19/16 , G06F19/706
摘要： A method of identifying, screening, characterising or designing a chemical entity, which mimics or binds to FIH, is described. The method comprises comparing a structural model of FIH with a structural model for said chemical entity, wherein said structural model of FIH is derived from structural factors or structural coordinates determined by subjecting to X-ray diffraction measurements a crystal comprising FIH. Such chemical entities may be used in the treatment of a condition associated with increased or decreased HIF levels or activity.
摘要翻译： 描述了识别，筛选，表征或设计模拟或结合FIH的化学实体的方法。 该方法包括将FIH的结构模型与所述化学实体的结构模型进行比较，其中FIH的结构模型来源于通过对包含FIH的晶体进行X射线衍射测量确定的结构因子或结构坐标。 这些化学实体可用于治疗与增加或降低的HIF水平或活性相关的病症。
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3.发明授权Assays for identifying modulators of the hydroxylation of ankyrin repeat proteins by 2-oxoglutarate dependent oxygenase and methods of using the same 失效通过2-氧戊二酸依赖性加氧酶鉴定锚蛋白重复蛋白质羟基化的调节剂及其使用方法公开(公告)号：US07638295B2
公开(公告)日：2009-12-29
申请号：US10594295
申请日：2005-03-24
申请人： Christopher Joseph Schofield , Kirsty Sarah Hewitson , Michael Arnold McDonough , Peter John Ratcliffe , Norma Masson , Matthew Edward Cockman
发明人： Christopher Joseph Schofield , Kirsty Sarah Hewitson , Michael Arnold McDonough , Peter John Ratcliffe , Norma Masson , Matthew Edward Cockman
CPC分类号： C12Q1/26 , G01N2333/90245 , G01N2500/04
摘要： A method of identifying an agent which modulates 2-oxoglutarate dependent oxygenase activity, the method comprising contacting a 2-oxoglutarate dependent oxygenase and a test agent in the presence of a substrate comprising one or more ankyrin repeat, or fragment thereof, in conditions under which the substrate is hydroxylated in the absence of the test agent; and determining hydroxylation of the substrate.
摘要翻译： 鉴定调节2-氧戊酸依赖性加氧酶活性的试剂的方法，所述方法包括在包含一种或多种锚蛋白重复序列或其片段的底物存在下使2-氧戊二酸依赖性加氧酶和测试试剂接触， 在没有试剂的情况下将底物羟基化; 并测定底物的羟基化。
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4.发明授权Methods for expressing nucleic acid sequences using nucleic acid constructs comprising hypoxia response elements 失效使用包含缺氧应答元件的核酸构建体表达核酸序列的方法公开(公告)号：US06265390B1
公开(公告)日：2001-07-24
申请号：US09253738
申请日：1999-02-22
申请人： Peter John Ratcliffe , John David Firth , Adrian Llewllyn Harris , Christopher William Pugh , Ian James Stratford
发明人： Peter John Ratcliffe , John David Firth , Adrian Llewllyn Harris , Christopher William Pugh , Ian James Stratford
IPC分类号： A01N4304
CPC分类号： C12N9/1217 , A61K38/45 , A61K38/50 , A61K48/00 , C12N15/63 , C12N15/85 , C12N2830/002 , C12N2830/85
摘要： Nucleic acid constructs comprising hypoxia response elements in operable linkage with a coding sequence of a gene of interest, and methods for expressing a nucleic acid sequence using the constructs, are disclosed. In particular, such nucleic acid constructs comprise genes encoding prodrug activation systems or cytokines.
摘要翻译： 公开了包含与感兴趣的基因的编码序列可操作连接的缺氧应答元件的核酸构建体，以及使用该构建体表达核酸序列的方法。 特别地，这样的核酸构建体包含编码前体药物活化系统或细胞因子的基因。
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公开(公告)号：US5942434A
公开(公告)日：1999-08-24
申请号：US693174
申请日：1996-12-12
申请人： Peter John Ratcliffe , John David Firth , Adrian Llewllyn Harris , Christopher William Pugh , Ian James Stratford
发明人： Peter John Ratcliffe , John David Firth , Adrian Llewllyn Harris , Christopher William Pugh , Ian James Stratford
IPC分类号： C12N15/09 , A61K31/70 , A61K35/76 , A61K38/00 , A61K38/45 , A61K38/50 , A61K48/00 , A61P35/00 , A61P43/00 , C07H21/04 , C12N9/12 , C12N9/78 , C12N15/63 , C12N15/85 , C12R1/91 , C12N15/00 , A01N43/04
CPC分类号： C12N9/1217 , A61K38/45 , A61K38/50 , A61K48/00 , C12N15/63 , C12N15/85 , C12N2830/002 , C12N2830/85
摘要： Nucleic acid constructs comprising hypoxia response elements in operable linkage with a coding sequence of a gene of interest are disclosed. In particular, such nucleic acid constructs comprise genes encoding pro-drug activation systems or cytokines.
摘要翻译： PCT No.PCT / GB95 / 00322 Sec。 371日期：1996年12月12日 102（e）日期1996年12月12日PCT提交1995年2月15日PCT公布。 公开号WO95 / 21927 日期：1995年8月17日公开了包含与感兴趣的基因的编码序列可操作连锁的缺氧应答元件的核酸构建体。 特别地，这样的核酸构建体包含编码前药活化系统或细胞因子的基因。
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公开(公告)号：US09228224B2
公开(公告)日：2016-01-05
申请号：US13985243
申请日：2012-02-10
申请人： Christopher Joseph Schofield , Alexander Wolf , Wei Ge , Armin Thalhammer , Christoph Loenarz , Peter John Ratcliffe , Matthew Edward Cockman
发明人： Christopher Joseph Schofield , Alexander Wolf , Wei Ge , Armin Thalhammer , Christoph Loenarz , Peter John Ratcliffe , Matthew Edward Cockman
IPC分类号： C12Q1/26
CPC分类号： C12Q1/26 , G01N2333/90245
摘要： The present invention relates to assays for monitoring activity of OGFOD1 activity, in particular, to assays for identifying modulators of OGFOD1 activity. The invention also relates to assays to monitor the prolyl hydroxylase activity of OGFOD1 on its substrate, the human ribosomal protein RPS23. The invention also enables the introduction of 3-hydroxyprolyl residues into peptides and proteins.
摘要翻译： 本发明涉及用于监测OGFOD1活性的活性的测定法，特别是用于鉴定OGFOD1活性调节剂的测定法。 本发明还涉及监测OGFOD1在其底物，人核糖体蛋白RPS23上的脯氨酰羟化酶活性的测定。 本发明还能够将3-羟基脯氨酸残基引入肽和蛋白质。
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公开(公告)号：US20140248636A1
公开(公告)日：2014-09-04
申请号：US14235217
申请日：2012-07-25
申请人： Christopher Joseph Schofield , Wei Ge , Matthew Edward Cockman , Peter John Ratcliffe , Mathew Louis Coleman
发明人： Christopher Joseph Schofield , Wei Ge , Matthew Edward Cockman , Peter John Ratcliffe , Mathew Louis Coleman
IPC分类号： C12Q1/26 , G01N33/573
CPC分类号： C12Q1/26 , G01N33/5023 , G01N33/573 , G01N2333/90245
摘要： The present invention relates to assays for monitoring activity of Mina53 and NO66 activities, in particular, to assays for identifying modulators of Mina53 and NO66 activities. The invention also relates to assays to monitor the histidinyl hydroxylase activity of Mina53 and NO66 on their substrates, the human ribosomal protein Rpl27a and Rpl8 respectively. The invention also enables the introduction of S-3-hydroxyhistidinyl residues into peptides and proteins.
摘要翻译： 本发明涉及用于监测Mina53和NO66活性的活性的测定法，特别涉及鉴定Mina53和NO66活性的调节剂的测定。 本发明还涉及分别监测Mina53和NO66对其底物，人核糖体蛋白Rpl27a和Rpl8的组氨酸羟化酶活性的测定。 本发明还能够将S-3-羟基组氨酸残基引入肽和蛋白质。
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公开(公告)号：US08535899B2
公开(公告)日：2013-09-17
申请号：US12654993
申请日：2010-01-12
申请人： Patrick Henry Maxwell , Christopher William Pugh , Peter John Ratcliffe , Christopher Joseph Schofield
发明人： Patrick Henry Maxwell , Christopher William Pugh , Peter John Ratcliffe , Christopher Joseph Schofield
IPC分类号： C12Q1/34 , C12Q1/26 , G01N33/573 , C12N9/14
CPC分类号： A61K31/455 , A01K2217/05 , A61K31/137 , A61K31/14 , A61K31/165 , A61K31/166 , A61K31/185 , A61K31/192 , A61K31/194 , A61K31/195 , A61K31/197 , A61K31/198 , A61K31/21 , A61K31/221 , A61K31/223 , A61K31/225 , A61K31/235 , A61K31/24 , A61K31/265 , A61K31/327 , A61K31/44 , A61K31/4412 , A61K38/00 , A61K39/3955 , C07C235/80 , C07C323/60 , C07C327/32 , C07D213/80 , C07D213/81 , C07D213/82 , C07K14/4702 , C07K16/40 , C07K2317/30 , C12N9/0071 , C12Q1/26 , C12Q1/34 , G01N33/573 , G01N2333/90245 , G01N2500/04 , G01N2500/20
摘要： A novel class of hydroxylases is described having the amino acid sequence of SEQ ID NO: 2, 4, 6 and 8, and variants and fragments thereof having HIF hydroxylation activity. The polypeptides of the invention have in particular prolyl hydroxylase activity. An assay method monitors the interaction of the HIF hydroxylase with a substrate. Modulators of HIF hydroxylase are provided for use in the treatment of a condition associated with increased or decreased HIF levels or activity or for the treatment of a condition where it is desirable to modulate HIF levels or activity.
摘要翻译： 描述了一类新型的羟化酶，其具有SEQ ID NO：2,4,6和8的氨基酸序列，其变体和片段具有HIF羟基化活性。 本发明的多肽特别是脯氨酰羟化酶活性。 测定方法监测HIF羟化酶与底物的相互作用。 提供HIF羟化酶的调节剂用于治疗与增加或降低的HIF水平或活性有关的病症，或用于治疗期望调节HIF水平或活性的病症。
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9.发明申请Interaction Between the VHL Tumour Suppressor and Hypoxia Inducible Factor, and Assay Methods Relating Thereto 审中-公开VHL肿瘤抑制因子与缺氧诱导因子之间的相互作用及与其相关的检测方法公开(公告)号：US20100330668A1
公开(公告)日：2010-12-30
申请号：US12726145
申请日：2010-03-17
CPC分类号： C07K14/4703 , A61K38/00 , C07K14/4702 , C07K2319/00
摘要： The invention relates to the finding that the VHL tumour suppressor protein regulates hypoxia inducible factor α subunits, by targeting HIF α for destruction in normoxic, but not hypoxic cells. The invention provides assays for modulators of this interaction, and peptides based upon HIF α subunit sequences which may modulate this interaction.
摘要翻译： 本发明涉及VHL肿瘤抑制蛋白调节缺氧诱导因子α亚基的发现，通过靶向HIFα在正常氧而不是缺氧细胞中的破坏。 本发明提供了这种相互作用的调节剂的测定法，以及基于可以调节这种相互作用的HIFα亚基序列的肽。
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10.发明授权Interaction between the VHL tumor suppressor and hypoxia inducible factor, and assay methods relating thereto 有权VHL肿瘤抑制因子和缺氧诱导因子之间的相互作用以及与之相关的测定方法公开(公告)号：US07709600B2
公开(公告)日：2010-05-04
申请号：US10901583
申请日：2004-07-29
IPC分类号： C07K5/00
CPC分类号： C07K14/4703 , A61K38/00 , C07K14/4702 , C07K2319/00
摘要： The invention relates to the finding that the VHL tumour suppressor protein regulates hypoxia inducible factor α subunits, by targeting HIF α for destruction in normoxic, but not hypoxic cells. The invention provides assays for modulators of this interaction, and peptides based upon HIF α subunit sequences which may modulate this interaction.
摘要翻译： 本发明涉及VHL肿瘤抑制蛋白调节缺氧诱导因子α亚基的发现，通过靶向HIFα在正常氧而不是缺氧细胞中的破坏。 本发明提供了这种相互作用的调节剂的测定法，以及基于可以调节这种相互作用的HIFα亚基序列的肽。
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