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11 条记录 (耗时 0.016 秒)

    Method for purifying a target nucleic acid
    1.
    发明授权
    Method for purifying a target nucleic acid 有权

    公开(公告)号：US09624253B2

    公开(公告)日：2017-04-18

    申请号：US13810406

    申请日：2011-07-15

    申请人： Thorsten Voss ,  Ralf Wyrich

    发明人： Thorsten Voss ,  Ralf Wyrich

    IPC分类号： C07H1/08 ,  C12N15/10

    CPC分类号： C07H1/08 ,  C12N15/1003 ,  C12N15/1006

    摘要： The present invention pertains to method for purifying at least a target nucleic acid from a sample, said method comprising at least the following steps: a) incubating the sample with at least one protein-degrading compound; b) binding the target nucleic acid to a solid phase; c) eluting the target nucleic acid from the solid phase; d) incubating the eluted target nucleic acid with at least one protein-degrading compound; e) binding the target nucleic acid again to a solid phase; f) optionally eluting the bound target nucleic acid from the solid phase. It was surprisingly found that performing a second protein digestion step after the target nucleic acid was bound and eluted from a solid phase before the nucleic acids are rebound to a solid phase is very efficient in reducing remaining protein contaminations in the isolated nucleic acid.

    METHOD FOR PURIFYING A TARGET NUCLEIC ACID
    4.
    发明申请
    METHOD FOR PURIFYING A TARGET NUCLEIC ACID 有权
    净化目标核酸的方法

    公开(公告)号：US20130122574A1

    公开(公告)日：2013-05-16

    申请号：US13810406

    申请日：2011-07-15

    申请人： Thorsten Voss ,  Ralf Wyrich

    发明人： Thorsten Voss ,  Ralf Wyrich

    IPC分类号： C07H1/08

    CPC分类号： C07H1/08 ,  C12N15/1003 ,  C12N15/1006

    摘要： The present invention pertains to method for purifying at least a target nucleic acid from a sample, said method comprising at least the following steps: a) incubating the sample with at least one protein-degrading compound; b) binding the target nucleic acid to a solid phase; c) eluting the target nucleic acid from the solid phase; d) incubating the eluted target nucleic acid with at least one protein-degrading compound; e) binding the target nucleic acid again to a solid phase; f) optionally eluting the bound target nucleic acid from the solid phase. It was surprisingly found that performing a second protein digestion step after the target nucleic acid was bound and eluted from a solid phase before the nucleic acids are rebound to a solid phase is very efficient in reducing remaining protein contaminations in the isolated nucleic acid.

    摘要翻译： 本发明涉及从样品中纯化至少一种靶核酸的方法，所述方法至少包括以下步骤：a）将所述样品与至少一种蛋白质降解化合物一起孵育; b）将靶核酸与固相结合; c）从固相中洗脱靶核酸; d）将洗脱的目标核酸与至少一种蛋白质降解化合物孵育; e）将靶核酸再次结合到固相; f）任选地从固相中洗脱结合的靶核酸。 惊奇地发现，在核酸反弹到固相之前，在靶核酸结合和洗脱之后进行第二蛋白质消化步骤对于减少分离的核酸中剩余的蛋白质污染物是非常有效的。

    Compositions for isolating and/or stabilising nucleic acids in biological material
    5.
    发明授权
    Compositions for isolating and/or stabilising nucleic acids in biological material 有权
    用于在生物材料中分离和/或稳定核酸的组合物

    公开(公告)号：US07270953B2

    公开(公告)日：2007-09-18

    申请号：US10312745

    申请日：2001-05-22

    申请人： Vera Holländer ,  Ralf Wyrich ,  Uwe Oelmüller

    发明人： Vera Holländer ,  Ralf Wyrich ,  Uwe Oelmüller

    IPC分类号： C12Q1/68 ,  C07H21/00 ,  C07H21/04

    CPC分类号： C12N15/1003 ,  C07C211/62 ,  C07C211/63 ,  C07C211/64 ,  C07F9/54

    摘要： The present invention relates to new compositions for isolating and/or stabilising nucleic acids in materials of biological origin. The compositions comprise as an essential ingredient a cationic compound of general formula Y+R1R2R3R4X− wherein Y may represent nitrogen or phosphorus R1, R2, R3 and R4 independently of one another may represent a branched or unbranched C1-C20-alkyl group and/or a C6-C20-aryl group as well as a C6-C26-aralkyl group and X− may represent an anion of an inorganic or organic, mono- or polybasic acid.

    摘要翻译： 本发明涉及用于在生物来源的材料中分离和/或稳定核酸的新组合物。 组合物包含作为必要成分的通式为“-in-line-formula”的阳离子化合物=“In-line formula”end =“lead”→> Y < ？In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In In 其中Y可以表示氮或磷R 1，R 2，R 3和R 3， 彼此独立地可以表示支链或非支链C 1 -C 20 - 烷基和/或C 6 - C 1 -C 20 - 芳基以及C 6 -C 26 - 芳烷基和X - O - >可以代表无机或有机单或多元酸的阴离子。

    Method and device for collecting and stabilizing a biological sample
    6.
    发明授权
    Method and device for collecting and stabilizing a biological sample 有权
    用于收集和稳定生物样品的方法和装置

    公开(公告)号：US06821789B2

    公开(公告)日：2004-11-23

    申请号：US10610554

    申请日：2003-07-02

    申请人： Frank A. Augello ,  Lynne Rainen ,  Matthew Walenciak ,  Uwe Oelmüller ,  Ralf Wyrich ,  Helge Bastian

    发明人： Frank A. Augello ,  Lynne Rainen ,  Matthew Walenciak ,  Uwe Oelmüller ,  Ralf Wyrich ,  Helge Bastian

    IPC分类号： G01N100

    CPC分类号： G01N33/96 ,  C12Q1/6806 ,  Y10T436/10 ,  Y10T436/108331 ,  Y10T436/2525 ,  C12Q2527/125 ,  C12Q2527/127

    摘要： A collection container and method for collecting a predetermined volume of a biological sample, and particularly a whole blood sample, includes at least one gene induction blocking agent in an amount effective to stabilize and inhibit gene induction. The gene induction blocking agent is able to stabilize nucleic acids in the biological sample at the point of collection to block ex vivo gene induction in the sample when stored at room temperature. The stabilizing agents include cationic compounds, detergents, particularly cationic detergents, chaotropic salts, ribonuclease inhibitors, chelating agents, organic solvents, organic reducing reagents, and mixtures thereof. The biological sample is collected directly from the animal and immediately mixed with the gene induction blocking agent without any intermediate processing or handling.

    摘要翻译： 用于收集预定体积的生物样品，特别是全血样品的收集容器和方法包括至少一种有效稳定和抑制基因诱导的基因诱导阻断剂。 基因诱导阻断剂能够在收集时稳定生物样品中的核酸，以在室温下储存时阻断样品中的离体基因诱导。 稳定剂包括阳离子化合物，洗涤剂，特别是阳离子洗涤剂，离液盐，核糖核酸酶抑制剂，螯合剂，有机溶剂，有机还原剂及其混合物。 生物样品直接从动物中收集，并立即与基因诱导阻断剂混合，无需任何中间加工或处理。

    METHOD FOR ISOLATING SMALL RNA
    8.
    发明申请
    METHOD FOR ISOLATING SMALL RNA 审中-公开
    分离小RNA的方法

    公开(公告)号：US20120295328A1

    公开(公告)日：2012-11-22

    申请号：US13522659

    申请日：2011-01-18

    申请人： Ralf Wyrich ,  Thorsten Voss

    发明人： Ralf Wyrich ,  Thorsten Voss

    IPC分类号： C07H1/08 ,  C09K3/00 ,  C07H21/02 ,  C12N9/50 ,  C12S99/00

    CPC分类号： C12N15/1006 ,  C12N15/1003

    摘要： A method for isolating small RNA from a sample is provided, the method comprising binding the RNA to silica particles by contacting the sample with a) at least one alcohol, b) at least one chaotropic salt comprising a chaotropic anion selected from the group consisting of trichloroacetate, perchlorate and trifluoroacetate and c) silica particles and separating the bound RNA from the rest of the sample. The present invention also provides compositions and kits to efficiently isolate small RNA molecules from samples, in particular biological samples such as blood, blood products tissue and body fluids.

    摘要翻译： 提供了从样品中分离小RNA的方法，所述方法包括通过使样品与a）至少一种醇接触来将RNA结合到二氧化硅颗粒上，b）至少一种离液序列盐，其包含选自以下的离液序列： 三氯乙酸盐，高氯酸盐和三氟乙酸盐，和c）二氧化硅颗粒，并将结合的RNA与样品的其余部分分离。 本发明还提供了用于从样品，特别是生物样品如血液，血液制品组织和体液中有效分离小RNA分子的组合物和试剂盒。

    Method of stabilizing and/or isolating nucleic acids
    9.
    发明授权
    Method of stabilizing and/or isolating nucleic acids 有权
    稳定和/或分离核酸的方法

    公开(公告)号：US07683035B1

    公开(公告)日：2010-03-23

    申请号：US09510534

    申请日：2000-02-22

    申请人： Christoph Erbacher ,  Helge Bastian ,  Ralf Wyrich ,  Uwe Oelmüller ,  Thomas Manz

    发明人： Christoph Erbacher ,  Helge Bastian ,  Ralf Wyrich ,  Uwe Oelmüller ,  Thomas Manz

    IPC分类号： A01N43/04 ,  A61K31/70 ,  G01N1/00 ,  A61K9/127 ,  C12Q1/68 ,  C12N5/00 ,  C12N15/88

    CPC分类号： C12N15/1003 ,  Y10T436/2525

    摘要： The present invention relates to a method of stabilizing and/or isolating nucleic acids, wherein a biological sample containing nucleic acids is contacted with a cationic compound. The invention also relates to said cationic compound per se and to the use of said cationic compound in stabilizing and/or isolating nucleic acids. Furthermore, the invention relates to pharmaceutical compositions, diagnostic compositions, and to compositions used in research, which include cationic compounds or a complex being formed upon contact of said cationic compound with a nucleic acid.

    摘要翻译： 本发明涉及一种稳定和/或分离核酸的方法，其中含有核酸的生物样品与阳离子化合物接触。 本发明还涉及所述阳离子化合物本身以及所述阳离子化合物在稳定和/或分离核酸中的用途。 此外，本发明涉及药物组合物，诊断组合物和用于研究的组合物，其包括阳离子化合物或在所述阳离子化合物与核酸接触时形成的络合物。

    Compositions for the isolation and/or stabilization of nucleic acids in biological materials
    10.
    发明授权
    Compositions for the isolation and/or stabilization of nucleic acids in biological materials 有权
    生物材料中核酸分离和/或稳定化的组合物

    公开(公告)号：US07682790B2

    公开(公告)日：2010-03-23

    申请号：US11890415

    申请日：2007-08-06

    申请人： Vera Holländer ,  Ralf Wyrich ,  Uwe Oelmüller

    发明人： Vera Holländer ,  Ralf Wyrich ,  Uwe Oelmüller

    IPC分类号： C12Q1/68 ,  C07H21/00 ,  C07H21/02

    CPC分类号： C12N15/1003 ,  C07C211/62 ,  C07C211/63 ,  C07C211/64 ,  C07F9/54

    摘要： The present invention relates to new compositions for isolating and/or stabilizing nucleic acids in materials of biological origin. The compositions comprise as an essential ingredient a cationic compound of general formula Y+R1R2R3R4X− wherein Y may represent nitrogen or phosphorus R1, R2, R3 and R4 independently of one another may represent a branched or unbranched C1-C20-alkyl group and/or a C6-C20-aryl group as well as a C6-C26-aralkyl group and X− may represent an anion of an inorganic or organic, mono- or polybasic acid.

    摘要翻译： 本发明涉及用于在生物来源的材料中分离和/或稳定核酸的新组合物。 组合物包含通式Y + R1R2R3R4X-的阳离子化合物，其中Y可以表示氮或磷，R 1，R 2，R 3和R 4彼此独立地表示支链或非支链C 1 -C 20 - 烷基和/或 C 6 -C 20 - 芳基以及C 6 -C 26 - 芳烷基，X - 可以表示无机或有机的单或多元酸的阴离子。