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公开(公告)号：US20110206576A1
公开(公告)日：2011-08-25
申请号：US13057958
申请日：2009-08-04
Applicant: Tim Woudenberg , Jing Wang , Hou-Pu Chou
Inventor： Tim Woudenberg , Jing Wang , Hou-Pu Chou
CPC classification number: B01L3/502715 , B01F13/0059 , B01F13/0094 , B01F13/1013 , B01F13/1022 , B01J2219/00317 , B01J2219/00479 , B01J2219/00585 , B01L3/5025 , B01L3/502723 , B01L3/502738 , B01L3/502746 , B01L7/52 , B01L2200/027 , B01L2200/0605 , B01L2300/0829 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0887 , B01L2300/123 , B01L2300/1805 , B01L2400/0487 , B01L2400/0605 , B01L2400/0655 , B01L2400/082 , B33Y80/00 , G01N1/28 , H04W4/70 , H04W76/30
Abstract: Microfluidic devices are described that include a rigid base layer, and an elastomeric layer on the base layer. The elastomeric layer may include at least part of a fluid channel for transporting a liquid reagent, and a vent channel that accepts gas diffusing through the elastomeric layer from the flow channel and vents it out of the elastomeric layer. The devices may also include a mixing chamber fluidly connected to the fluid channel, and a control channel overlapping with a deflectable membrane that defines a portion of the flow channel, where the control channel may be operable to change a rate at which the liquid reagent flows through the fluid channel. The devices may further include a rigid plastic layer on the elastomeric layer.
Abstract translation: 描述了包括刚性基层和基层上的弹性体层的微流体装置。 弹性体层可以包括用于输送液体试剂的流体通道的至少一部分，以及排气通道，其接纳从流动通道穿过弹性体层扩散的气体并将其从弹性体层排出。 装置还可以包括流体连接到流体通道的混合室，以及与限定流动通道的一部分的可偏转膜重叠的控制通道，其中控制通道可操作以改变液体试剂流动的速率 通过流体通道。 所述装置还可包括弹性体层上的刚性塑料层。
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公开(公告)号：US20050233363A1
公开(公告)日：2005-10-20
申请号：US11096282
申请日：2005-03-31
Applicant: Ian Harding , Donald Sandell , Kevin Bodner , Tim Woudenberg , Mark Oldham , H. Kao , Gary Lim , John Bodeau , Adrian Fawcett , Robert Jones
Inventor： Ian Harding , Donald Sandell , Kevin Bodner , Tim Woudenberg , Mark Oldham , H. Kao , Gary Lim , John Bodeau , Adrian Fawcett , Robert Jones
CPC classification number: C12N15/1072 , B01L3/50851 , B01L3/50853 , B01L7/52 , B01L2200/0689 , B01L2300/021 , B01L2300/041 , B01L2300/0829 , B01L2300/12 , B01L2300/1805
Abstract: A method for simultaneously determining a genetic expression profile for an individual member of a species relative to an entire standard genome for the species. The method can comprise distributing a liquid sample into an array of reaction chambers of a substrate. The array can comprise a primer set and a probe for each polynucleotide target along the entire standard genome. The liquid sample can comprise substantially all genetic material of the member. Each of the reaction chambers can comprise the primer set and the probe for at least one of the polynucleotide targets and a polymerase. The method can further comprise amplifying the liquid sample in the array, detecting a signal emitted by at least one of the probes, and identifying the genetic expression profile in response to the signal.
Abstract translation: 一种相对于物种的整个标准基因组同时测定物种的个体成员的遗传表达谱的方法。 该方法可以包括将液体样品分配到基底的反应室的阵列中。 该阵列可以包含沿着整个标准基因组的每个多核苷酸靶的引物组和探针。 液体样品可以包括构件的基本上所有的遗传物质。 每个反应室可以包含用于至少一个多核苷酸靶标和聚合酶的引物组和探针。 该方法还可以包括放大阵列中的液体样品，检测由至少一个探针发射的信号，以及响应于该信号鉴定遗传表达谱。
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公开(公告)号：US08617488B2
公开(公告)日：2013-12-31
申请号：US13057958
申请日：2009-08-04
Applicant: Tim Woudenberg , Jing Wang , Hou-Pu Chou
Inventor： Tim Woudenberg , Jing Wang , Hou-Pu Chou
IPC: G01N15/06
CPC classification number: B01L3/502715 , B01F13/0059 , B01F13/0094 , B01F13/1013 , B01F13/1022 , B01J2219/00317 , B01J2219/00479 , B01J2219/00585 , B01L3/5025 , B01L3/502723 , B01L3/502738 , B01L3/502746 , B01L7/52 , B01L2200/027 , B01L2200/0605 , B01L2300/0829 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0887 , B01L2300/123 , B01L2300/1805 , B01L2400/0487 , B01L2400/0605 , B01L2400/0655 , B01L2400/082 , B33Y80/00 , G01N1/28 , H04W4/70 , H04W76/30
Abstract: Microfluidic devices are described that include a rigid base layer, and an elastomeric layer on the base layer. The elastomeric layer may include at least part of a fluid channel for transporting a liquid reagent, and a vent channel that accepts gas diffusing through the elastomeric layer from the flow channel and vents it out of the elastomeric layer. The devices may also include a mixing chamber fluidly connected to the fluid channel, and a control channel overlapping with a deflectable membrane that defines a portion of the flow channel, where the control channel may be operable to change a rate at which the liquid reagent flows through the fluid channel. The devices may further include a rigid plastic layer on the elastomeric layer.
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公开(公告)号：US20090176661A1
公开(公告)日：2009-07-09
申请号：US12407234
申请日：2009-03-19
Applicant: Ian A. Harding , Donald Sandell , Kevin Bodner , Tim Woudenberg , Mark Oldham , H. Pin Kao , Gary Lim , John P. Bodeau , Adrian Fawcett , Robert C. Jones
Inventor： Ian A. Harding , Donald Sandell , Kevin Bodner , Tim Woudenberg , Mark Oldham , H. Pin Kao , Gary Lim , John P. Bodeau , Adrian Fawcett , Robert C. Jones
IPC: C40B30/10
CPC classification number: C12N15/1072 , B01L3/50851 , B01L3/50853 , B01L7/52 , B01L2200/0689 , B01L2300/021 , B01L2300/041 , B01L2300/0829 , B01L2300/12 , B01L2300/1805
Abstract: A method for simultaneously determining a genetic expression profile for an individual member of a species relative to an entire standard genome for the species. The method can comprise distributing a liquid sample into an array of reaction chambers of a substrate. The array can comprise a primer set and a probe for each polynucleotide target along the entire standard genome. The liquid sample can comprise substantially all genetic material of the member. Each of the reaction chambers can comprise the primer set and the probe for at least one of the polynucleotide targets and a polymerase. The method can further comprise amplifying the liquid sample in the array, detecting a signal emitted by at least one of the probes, and identifying the genetic expression profile in response to the signal.
Abstract translation: 一种相对于物种的整个标准基因组同时测定物种的个体成员的遗传表达谱的方法。 该方法可以包括将液体样品分配到基底的反应室的阵列中。 该阵列可以包含沿着整个标准基因组的每个多核苷酸靶的引物组和探针。 液体样品可以包括构件的基本上所有的遗传物质。 每个反应室可以包含用于至少一个多核苷酸靶标和聚合酶的引物组和探针。 该方法还可以包括放大阵列中的液体样品，检测由至少一个探针发射的信号，以及响应于该信号鉴定遗传表达谱。
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