公开(公告)号：US20170009200A1

公开(公告)日：2017-01-12

申请号：US15275586

申请日：2016-09-26

Applicant: AJINOMOTO CO., INC.

Inventor： Yoko KURIYAMA ,  Satoru OKAMOTO ,  Manabu KITAZAWA ,  Nao SUGIMOTO ,  Takuya MATSUMOTO

IPC: C12N5/00 ,  C12N5/074

CPC classification number: C12N5/0018 ,  C12N5/0696 ,  C12N2500/50 ,  C12N2501/998

Abstract: Culture media which contain a water-soluble polymer, such as polyvinyl alcohol and the like, and albumin, and the like, are useful for the proliferation of stem cells, and show good culture results, particularly with iPS cells, and maintain the stability of the medium properties, as well as using a reduced amount of albumin.

Abstract translation: 含有水溶性聚合物如聚乙烯醇等的培养基和白蛋白等可用于干细胞的增殖，并且显示出良好的培养结果，特别是对于iPS细胞，并保持稳定性 中等特性，以及使用减少量的白蛋白。

公开(公告)号：US20150011003A1

公开(公告)日：2015-01-08

申请号：US14497926

申请日：2014-09-26

Applicant: AJINOMOTO CO., INC.

Inventor： Yoko KURIYAMA ,  Nao SUGIMOTO ,  Manabu KITAZAWA ,  Satoru OKAMOTO ,  Sho SENDA ,  Ikue HARATA ,  Satoru OHASHI

IPC: C12N5/0775 ,  C08G63/91 ,  C08G65/48 ,  C12N5/074

CPC classification number: C12N5/0663 ,  C08G63/912 ,  C08G65/48 ,  C12N5/0696 ,  C12N2500/34 ,  C12N2501/115

Abstract: A medium which comprises a fibroblast growth factor (FGF), and a sulfated compound or a pharmaceutically acceptable salt thereof at a concentration which promotes the growth of a stem cell in the presence of FGF, is useful for culturing stem cells.

Abstract translation: 包含成纤维细胞生长因子（FGF）的培养基和在FGF存在下促进干细胞生长的浓度的硫酸化合物或其药学上可接受的盐可用于培养干细胞。

公开(公告)号：US20150329832A1

公开(公告)日：2015-11-19

申请号：US14813374

申请日：2015-07-30

Applicant: AJINOMOTO CO., INC.,

Inventor： Sho SENDA ,  Tomomi YOSHIDA ,  Satoru OKAMOTO ,  Yoko KURIYAMA ,  Manabu KITAZAWA ,  lkue HARADA ,  Nao SUGIMOTO

IPC: C12N5/074

Abstract: Cultivating a pluripotent stem cell in a medium comprising at least one member selected from the group consisting of ethanolamine, an ethanolamine analog, and a pharmaceutically acceptable salt thereof, and which is substantially free of β-mercaptoethanol or contains β-mercaptoethanol at a concentration of not more than 9 μM, and the like, is effective for the proliferation of a pluripotent stem cell while maintaining an undifferentiated state.

Abstract translation: 在包含至少一种选自乙醇胺，乙醇胺类似物和其药学上可接受的盐的培养基中培养多能干细胞，其基本上不含β-巯基乙醇或含有β-巯基乙醇 不超过9μM的浓度等对多能干细胞的增殖有效，同时保持未分化状态。

公开(公告)号：US20190136202A1

公开(公告)日：2019-05-09

申请号：US16242463

申请日：2019-01-08

Applicant: AJINOMOTO CO., INC.,

Inventor： Sho SENDA ,  Tomomi YOSHIDA ,  Satoru OKAMOTO ,  Yoko KURIYAMA ,  Manabu KITAZAWA ,  Ikue HARATA ,  Nao SUGIMOTO

IPC: C12N5/074 ,  C12N5/0735 ,  C12N5/00

CPC classification number: C12N5/0696 ,  C12N5/0043 ,  C12N5/0606 ,  C12N2500/36 ,  C12N2500/46 ,  C12N2500/84 ,  C12N2533/90

Abstract: Cultivating a pluripotent stem cell in a medium comprising at least one member selected from the group consisting of ethanolamine, an ethanolamine analog, and a s pharmaceutically acceptable salt thereof, and which is substantially free of β-mercaptoethanol or contains β-mercaptoethanol at a concentration of not more than 9 μM, and the like, is effective for the proliferation of a pluripotent stem cell while maintaining an undifferentiated state.

公开(公告)号：US20200040308A1

公开(公告)日：2020-02-06

申请号：US16585184

申请日：2019-09-27

Applicant: AJINOMOTO CO., INC. ,  KEIO UNIVERSITY

Inventor： Kotoe KOSEKI ,  Satoru OKAMOTO ,  Shugo TOHYAMA ,  Jun FUJITA ,  Keiichi FUKUDA ,  Shota SOMEYA

IPC: C12N5/074

Abstract: Media which contain 4-tryptophan or an L-tryptophan derivative at a concentration of not less than 176 μM are useful for culturing pluripotent stem cells.