公开(公告)号：US20250101129A1

公开(公告)日：2025-03-27

申请号：US18966063

申请日：2024-12-02

Applicant: Ajinomoto Co., Inc.

Inventor： Yutaka MATSUDA ,  Tomohiro FUJII ,  Kenichiro ITO ,  Kazutoshi TAKAHASHI ,  Yoshihiko MATSUDA ,  Hiroki YAMAGUCHI ,  Naoko TSUYOSHI ,  Hayato NAGANO ,  Rika TAKASUGI

IPC: C07K16/32 ,  C07K16/28

Abstract: The present invention relates to a technology for easy chemical modification of only one heavy chain in the constituent unit of an antibody (immunoglobulin unit containing two heavy chains and optionally two light chains). More particularly, the present invention relates to a compound or a salt thereof, containing (A) an affinity substance containing first and second affinity moieties each having an affinity to the constant region in a heavy chain of an antibody; and (B) a reactive group for the antibody.

公开(公告)号：US20210300972A1

公开(公告)日：2021-09-30

申请号：US17241585

申请日：2021-04-27

Applicant: Ajinomoto Co., Inc.

Inventor： Kei YAMADA ,  Yoshihiko MATSUDA ,  Kazutoshi TAKAHASHI ,  Natsuki SHIKIDA ,  Kazutaka SHIMBO ,  Hayato NAGANO

IPC: C07K14/31 ,  C07K16/32

Abstract: Compounds having an affinity substance to an antibody, a cleavable portion, and a reactive group, or a salt thereof, are represented by the following Formula (I): A-L-B—R  (I) wherein A is an affinity substance to an antibody, L is a cleavable linker that is a divalent group comprising a cleavable portion, B is (a) a divalent group comprising a bioorthogonal functional group or (b) a divalent group comprising no bioorthogonal functional group, and R is a reactive group to the antibody, wherein the affinity substance to an antibody is a polypeptide comprising a glutamine residue (Q) at an N-terminal.

公开(公告)号：US20230265398A1

公开(公告)日：2023-08-24

申请号：US18191994

申请日：2023-03-29

Applicant: AJINOMOTO CO., INC.

Inventor： Kazutoshi TAKAHASHI ,  Takuto ONO ,  Nao ARAKI ,  Hiroaki KOBAYASHI

IPC: C12N9/10 ,  A23C19/08 ,  A23C19/06 ,  A23G9/36 ,  A23G3/36 ,  A23L13/40 ,  A23L13/60 ,  A23L15/00 ,  A23L17/00 ,  A23L29/00

CPC classification number: C12N9/1044 ,  C12Y203/02013 ,  A23C19/08 ,  A23C19/063 ,  A23G9/363 ,  A23G3/366 ,  A23L13/48 ,  A23L13/60 ,  A23L15/25 ,  A23L17/65 ,  A23L29/06

Abstract: A mutant transglutaminase (mutant TG) having high thermostability and/or high pH stability is provided. A mutant TG having a mutation at amino acid residue(s) such as D1, Y24, R48, S101, G102, N139, D142, L147, K152, G157, R167, N176, K181, E182, H188, D189, R208, T245, S246, G250, G275, S284, H289, G301, and K327 and/or a mutation of introducing a disulfide bond.