公开(公告)号：US20230042456A1

公开(公告)日：2023-02-09

申请号：US17786864

申请日：2020-12-16

Applicant: BASF SE

Inventor： Hartwig SCHROEDER ,  Anne-Catrin HAILER ,  Birgit HOFF ,  Oskar ZELDER ,  Peter OEDMAN ,  Georg Benjamin WANDREY ,  Christian DIETZSCH ,  Mukesh KUMAR ,  Dominik SATORY ,  Matthew David BLANKSCHIEN ,  Jens Klaus PLASSMEIER

IPC: C12P19/04 ,  C12N9/88

Abstract: Increasing space-time-yield, carbon-conversion-efficiency and carbon substrate flexibility in the production of fine chemicals The inventors of the current invention have found a surprising positive effect of increased cAMP levels and/or manipulating the PTS system on the space-time-yield, carbon-conversion-efficiency and carbon substrate flexibility of fine chemical production of a host organism. This was achieved by de-regulating adenylate cyclase cyaa by deleting the C-terminal regulatory region leading to increased cAMP levels or deleting the Crr protein activity (carbohydrate repression resistance) which regulates the carbohydrate utilization system. Both lead to increased 2-fucosyllactoe and 6-sialyllactose production (human milk oligosaccharides) and increase carbohydrate usage.

公开(公告)号：US20170369835A1

公开(公告)日：2017-12-28

申请号：US15533432

申请日：2015-12-09

Applicant: BASF SE

Inventor： Birgit HOFF ,  Stefan HAEFNER ,  Weol Kyu JEONG ,  Edzard SCHOLTEN

IPC: C12N1/06 ,  C12N1/08 ,  C12P19/34

CPC classification number: C12N1/06 ,  C12N1/08 ,  C12N2510/00 ,  C12N2523/00 ,  C12P19/34 ,  C12P25/00

Abstract: The present invention relates to a method for degrading DNA in a sample obtained by microbial fermentation or bio-transformation, comprising treating the sample with a combination of increased temperature and low pH. It also relates to a method for releasing DNA from a microbial cell, comprising incubating the microbial cell at a temperature of 45° C. to 55° C. for two to ten hours. Finally, the present invention provides a method for producing a product, comprising a step of releasing DNA from a microbial cell and degrading said DNA.

公开(公告)号：US20160298160A1

公开(公告)日：2016-10-13

申请号：US15100376

申请日：2014-12-04

Applicant: BASF SE

Inventor： Birgit HOFF ,  Andrea MOLT ,  Stefan HAEFNER ,  Oskar ZELDER

IPC: C12P25/00 ,  C12N15/80

CPC classification number: C12P25/00 ,  C12N15/80

Abstract: The present invention relates to a method of producing riboflavin in a genetically modified organism of the genus Eremothecium, wherein said genetic modification is linked to the fatty acid uptake and/or beta-oxidation pathway of said organism, comprising growing said organisms in a culture medium and isolating riboflavin from the culture medium. The invention further relates to a method of providing a riboflavin accumulating organism belonging to the genus Eremothecium by genetically modifying said organism, to organisms obtained by such a method, as well as the use of such genetically modified organisms for increasing the accumulation of riboflavin.

Abstract translation: 本发明涉及一种在Eremothecium的遗传修饰生物体中生产核黄素的方法，其中所述遗传修饰与所述生物体的脂肪酸摄取和/或β-氧化途径相关，包括在培养基中培养所述生物体 并从培养基中分离出核黄素。 本发明还涉及通过遗传修饰所述生物体，通过这种方法获得的生物体以及使用这种转基因生物来增加核黄素的积累来提供属于Eremothecium属的核黄素积聚生物体的方法。