公开(公告)号：US10022721B2

公开(公告)日：2018-07-17

申请号：US14470860

申请日：2014-08-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Sepehr Kiani ,  Joshua Blouwolff ,  Adnan Esmail ,  Jason Hung ,  Tony Hung ,  Adam R. Abate ,  Scott Powers ,  Pascaline Mary

IPC: B01L3/00 ,  C12N15/10 ,  C12Q1/68 ,  G01N27/00 ,  B01L7/00 ,  C12M1/00

Abstract: Methods and systems for manipulating drops in microfluidic channels are provided.

公开(公告)号：US20170128937A1

公开(公告)日：2017-05-11

申请号：US15410913

申请日：2017-01-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Tony Hung ,  Adnan Esmail ,  Sepehr Kiani

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/502784 ,  B01L2200/06 ,  B01L2200/0605 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/161 ,  B01L2300/1861 ,  B01L2400/0415 ,  B01L2400/0448 ,  B01L2400/0487

Abstract: The present invention generally pertains to a system for performing droplet inflation, and methods and kits comprising the same. The system comprises at least one microfluidic channel comprising one or more droplets flowing therein, one or more fluid reservoirs, one or more inflators, one or more inflator nozzles, and at least one mechanism for disrupting an interface between a droplet and a fluid. The present invention provides for the inflation of a relatively controlled volume of fluid into a droplet resulting in an increase in the volume of the droplet relative to its volume prior to inflation and, accordingly, dilution of the concentration of species, if any, previously present and emulsified in the droplet.

公开(公告)号：US10343167B2

公开(公告)日：2019-07-09

申请号：US15691196

申请日：2017-08-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Adnan Esmail ,  Tal Raz ,  John Healy ,  Tony Hung ,  Sepehr Kiani ,  Pascaline Mary

IPC: B01L3/00 ,  B01L7/00 ,  G01N35/08 ,  C12Q1/6869

Abstract: The present invention provides a microfluidic system, method and kit for performing assays. The system may comprise a microfluidic device and a detector, wherein the assay yields results that may be read by a detector and analyzed by the system. The assay may comprise one or more chemical or biological reaction against, or performed on, a sample or multiple samples. The sample(s) may become larger and/or smaller during the performance of the assay. The sample(s) may be present within a vehicle, or on a carrier within a vehicle, in the microfluidic device, and wherein the vehicle may become larger and/or smaller during the performance of the assay. The assay may be a cascading assay comprising a series of multiple assays, wherein each assay may be the same or different, and wherein each assay in the series of multiple assays may further comprise one or more process or step.

公开(公告)号：US10159977B2

公开(公告)日：2018-12-25

申请号：US15410913

申请日：2017-01-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Tony Hung ,  Adnan Esmail ,  Sepehr Kiani

IPC: B01L3/00

Abstract: The present invention generally pertains to a system for performing droplet inflation, and methods and kits comprising the same. The system comprises at least one microfluidic channel comprising one or more droplets flowing therein, one or more fluid reservoirs, one or more inflators, one or more inflator nozzles, and at least one mechanism for disrupting an interface between a droplet and a fluid. The present invention provides for the inflation of a relatively controlled volume of fluid into a droplet resulting in an increase in the volume of the droplet relative to its volume prior to inflation and, accordingly, dilution of the concentration of species, if any, previously present and emulsified in the droplet.

公开(公告)号：US10130950B2

公开(公告)日：2018-11-20

申请号：US15039637

申请日：2014-11-25

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Tony Hung ,  Sepehr Kiani ,  Scott Powers ,  Adnan Esmail

IPC: B01L3/00 ,  B01J19/00 ,  B01L7/00

Abstract: Systems and methods for confining droplets within a microfluidic channel as well as systems and methods for packing droplets are provided. More specifically, a system and method are provided for controlling the introduction and removal of oil into a microfluidic channel in order to control where drops are allowed to flow within that channel.

公开(公告)号：US10730045B2

公开(公告)日：2020-08-04

申请号：US16196337

申请日：2018-11-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Tony Hung ,  Adnan Esmail ,  Sepehr Kiani

IPC: B01L3/00

Abstract: The present invention generally pertains to a system for performing droplet inflation, and methods and kits comprising the same. The system comprises at least one microfluidic channel comprising one or more droplets flowing therein, one or more fluid reservoirs, one or more inflators, one or more inflator nozzles, and at least one mechanism for disrupting an interface between a droplet and a fluid. The present invention provides for the inflation of a relatively controlled volume of fluid into a droplet resulting in an increase in the volume of the droplet relative to its volume prior to inflation and, accordingly, dilution of the concentration of species, if any, previously present and emulsified in the droplet.

公开(公告)号：US10589274B2

公开(公告)日：2020-03-17

申请号：US15986718

申请日：2018-05-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Sepehr Kiani ,  Joshua Blouwolff ,  Adnan Esmail ,  Jason Hung ,  Tony Hung ,  Adam R. Abate ,  Scott Powers ,  Pascaline Mary

IPC: B01L3/00 ,  C12N15/10 ,  B01L7/00

Abstract: Methods and systems for manipulating drops in microfluidic channels are provided.

公开(公告)号：US09821312B2

公开(公告)日：2017-11-21

申请号：US14427404

申请日：2013-09-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Adnan Esmail ,  John Healy ,  Tony Hung ,  Sepehr Kiani ,  Pascaline Mary ,  Tal Raz

IPC: B01L3/00 ,  G01N35/08 ,  B01L7/00

CPC classification number: B01L3/502784 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/18 ,  B01L2300/1861 ,  B01L2400/0415 ,  B01L2400/0433 ,  B01L2400/0448 ,  B01L2400/088 ,  G01N35/08 ,  Y10T436/143333 ,  Y10T436/2575

Abstract: The present invention provides a microfluidic system, method and kit for performing assays. The system may comprise a microfluidic device and a detector, wherein the assay yields results that may be read by a detector and analyzed by the system. The assay may comprise one or more chemical or biological reaction against, or performed on, a sample or multiple samples. The sample(s) may become larger and/or smaller during the performance of the assay. The sample(s) may be present within a vehicle, or on a carrier within a vehicle, in the microfluidic device, and wherein the vehicle may become larger and/or smaller during the performance of the assay. The assay may be a cascading assay comprising a series of multiple assays, wherein each assay may be the same or different, and wherein each assay in the series of multiple assays may further comprise one or more process or step.