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公开(公告)号：US20250121372A1
公开(公告)日：2025-04-17
申请号：US18901984
申请日：2024-09-30
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kevin D. NESS , Christopher F. KELLY , Donald A. MASQUELIER
IPC: B01L3/00 , B01F23/41 , B01F33/3011 , B01L3/02 , B01L7/00 , C12Q1/6806 , G01N35/00 , G01N35/08 , G01N35/10
Abstract: A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed.
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公开(公告)号：US20210170412A1
公开(公告)日：2021-06-10
申请号：US17110095
申请日：2020-12-02
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kevin D. NESS , Donald A. MASQUELIER , Billy W. COLSTON, JR. , Benjamin J. HINDSON
IPC: B01L3/00 , B01F13/00 , B01L3/02 , B01L7/00 , G01N21/3563 , B01F3/08 , C12Q1/686 , G01N21/64 , B01F15/00 , B29C45/00 , G01N21/49
Abstract: Method of analysis. In the method, a first emulsion and a second emulsion substantially separated from one another by a spacer fluid may be formed. The first emulsion, the spacer fluid, and the second emulsion may be flowed in a channel from a fluid inlet to a fluid outlet of a heating and cooling station having two or more temperature-controlled zones, such that each emulsion is thermally cycled to promote amplification of a nucleic acid target in droplets of the emulsion. Amplification data may be collected from individual droplets of each emulsion downstream of the heating and cooling station. A level of the nucleic acid target present in each emulsion may be determined based on the amplification data collected from the individual droplets of the emulsion.
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公开(公告)号：US20190247856A1
公开(公告)日：2019-08-15
申请号：US16397774
申请日：2019-04-29
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kevin D. NESS , Christopher F. KELLY , Donald A. MASQUELIER
IPC: B01L3/00 , B01F3/08 , B01F13/00 , G01N35/08 , C12Q1/6806
Abstract: A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed.
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公开(公告)号：US20230287486A1
公开(公告)日：2023-09-14
申请号：US18055398
申请日：2022-11-14
Applicant: Bio-Rad Laboratories, Inc.
Inventor： John F. REGAN , Serge SAXONOV , Michael Y. LUCERO , Benjamin J. HINDSON , Phillip BELGRADER , Simant DUBE , Austin SO , Jeffrey Clark MELLEN , Nicholas Jack HEREDIA , Kevin D. NESS , Billy W. COLSTON, Jr.
IPC: C12Q1/6858 , C12Q1/683 , C12Q1/6883 , C12Q1/6806 , C12Q1/6827 , C12Q1/686
CPC classification number: C12Q1/6858 , C12Q1/683 , C12Q1/6883 , C12Q1/6806 , C12Q1/6827 , C12Q1/686 , C12Q2600/158 , C12Q2600/172 , C12Q2535/125
Abstract: Method of haplotype analysis. In an exemplary method, an aqueous phase containing nucleic acid may be partitioned into a plurality of discrete volumes. At least one allele sequence may be amplified in the volumes from each of a first polymorphic locus and a second polymorphic locus that exhibit sequence variation in the nucleic acid. At least one measure of co-amplification of allele sequences from both loci in the same volumes may be determined. A haplotype of the first and second loci may be selected based on the at least one measure of co-amplification.
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公开(公告)号：US20210031200A1
公开(公告)日：2021-02-04
申请号：US16995555
申请日：2020-08-17
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kevin D. NESS , Christopher F. KELLY , Donald A. MASQUELIER
IPC: B01L3/00 , B01F13/00 , B01F3/08 , G01N35/08 , C12Q1/6806
Abstract: A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed.
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Patent Agency Ranking
公开(公告)号：US20220355292A1
公开(公告)日：2022-11-10
申请号：US17750230
申请日：2022-05-20
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Benjamin J. HINDSON , Serge SAXONOV , Phillip BELGRADER , Kevin D. NESS , Michael Y. LUCERO , Billy W. COLSTON, JR.
Abstract: This invention provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some cases, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.
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公开(公告)号：US20210220829A1
公开(公告)日：2021-07-22
申请号：US17137274
申请日：2020-12-29
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Kevin D. NESS , Donald A. MASQUELIER , Billy W. COLSTON, JR. , Benjamin J. HINDSON
IPC: B01L3/00 , B01F13/00 , B01L3/02 , B01L7/00 , G01N21/3563 , B01F3/08 , C12Q1/686 , G01N21/64 , B01F15/00 , B29C45/00 , G01N21/49
Abstract: System for performing a flow-based assay. The system may comprise a droplet generator to produce an emulsion including droplets in a carrier fluid. The system also may comprise a thermocycler including two or more temperature-controlled zones and also including a channel connected to the droplet generator for receiving the emulsion. The channel may form a single-pass continuous fluid route traversing the temperature-controlled zones multiple times, such that droplets passing through the channel are thermally cycled. The system further may comprise a detection station downstream from the thermocycler and configured to detect a signal from the droplets after such droplets have been thermally cycled by passing through the channel.
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公开(公告)号：US20200086312A1
公开(公告)日：2020-03-19
申请号：US16667811
申请日：2019-10-29
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Amy L. HIDDESSEN , Donald A. MASQUELIER , Kevin D. NESS , Benjamin J. HINDSON , Anthony J. MAKAREWICZ, JR. , Erin R. CHIA , Billy W. COLSTON, JR. , Serge SAXONOV , Svilen S. TZONEV , Michael Y. LUCERO , Ryan T. KOEHLER
Abstract: Method of detecting a target nucleic acid. In an exemplary method, at least two thermal zones of different temperature may be created using a heating assembly. A first emulsion and a second emulsion may be formed. The first and second emulsions may be thermally cycled by passing them through tubing in a spaced relation to one another, with the tubing being wound around a central axis of the heating assembly and extending through each thermal zone multiple times. Thermally cycling may promote amplification of the target nucleic acid in droplets of each emulsion. Droplets of each emulsion may be passed through a detection channel located downstream of the tubing. Fluorescence may be detected from the droplets being passed through the detection channel.
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公开(公告)号：US20220362764A1
公开(公告)日：2022-11-17
申请号：US17750195
申请日：2022-05-20
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Benjamin J. HINDSON , Serge SAXONOV , Phillip BELGRADER , Kevin D. NESS , Michael Y. LUCERO , Billy W. COLSTON, JR. , Shawn Paul HODGES , Nicholas J. HEREDIA , Jeffrey Clark MELLEN , Camille Bodley TROUP , Paul WYATT
Abstract: The present disclosure provides methods and compositions for detecting polynucleotides in a sample and for quantifying polynucleotide load in a sample. The polynucleotides can be associated with a disease, disorder, or condition. In some applications, methylated DNA is quantified, e.g., in order to determine the load of polynucleotides in a sample. The present disclosure also provides methods and compositions for determining the load of fetal polynucleotides in a biological sample, e.g., the load of fetal polynucleotides (e.g., DNA, RNA) in maternal plasma. The present disclosure provides methods and compositions for detecting cellular processes such as cellular viability, growth rates, and infection rates. This disclosure also provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some embodiments, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.
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公开(公告)号：US20190255531A1
公开(公告)日：2019-08-22
申请号：US16404659
申请日：2019-05-06
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Benjamin J. HINDSON , Kevin D. NESS , Billy W. COLSTON, JR. , Fred P. MILANOVICH , Donald A. MASQUELIER
IPC: B01L3/00 , G01N21/64 , B01L7/00 , B01F15/00 , B29C45/00 , G01N21/49 , B01F13/00 , B01L3/02 , G01N21/3563 , C12Q1/686 , B01F3/08
Abstract: Method of performing a droplet-based assay. The method may include obtaining droplets encapsulated by an immiscible liquid and packed closely together in a monolayer, performing a reaction in the droplets while packed closely together in the monolayer; and collecting data related to an analyte from a plurality of the droplets while the droplets remain closely packed together in the monolayer.
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