公开(公告)号：US20230159917A1

公开(公告)日：2023-05-25

申请号：US18094278

申请日：2023-01-06

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Sida Wang ,  Patrick Michael Tegels ,  William T. Koederitz ,  Daniel Genord ,  Grey Parker ,  Brian Boniface ,  Katlyn Curtin Mehne ,  Alec William Hitchiner

IPC: C12N15/10 ,  B01L3/00 ,  B01L7/00 ,  G01F23/292 ,  C12N1/06 ,  B01L9/02 ,  G01N35/00 ,  C12Q1/6844

CPC classification number: C12N15/1096 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/502761 ,  B01L7/52 ,  C12N15/1093 ,  G01F23/292 ,  C12N1/06 ,  B01L3/502 ,  B01L3/52 ,  B01L9/02 ,  G01N35/00 ,  C12Q1/6844 ,  B01L2400/06 ,  B01L2300/123 ,  B01L2200/0647 ,  B01L2300/041 ,  B01L2300/0829 ,  B01L2300/0819 ,  B01L2300/1805 ,  B01L2300/047 ,  B01L2200/16 ,  B01L2300/06 ,  B01L2300/042 ,  B01L2300/0627 ,  B01L2300/0893 ,  B01L2300/1894 ,  B01L2400/043 ,  B01L2400/0633 ,  B01L2300/16

Abstract: A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements; a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including mRNA capture, cDNA synthesis, protein-associated assays, and library preparation, for next generation sequencing.

公开(公告)号：US11578322B2

公开(公告)日：2023-02-14

申请号：US16867256

申请日：2020-05-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Sida Wang ,  Patrick Michael Tegels ,  William T. Koederitz ,  Daniel Genord ,  Grey Parker ,  Brian Boniface ,  Katlyn Curtin Mehne ,  Alec William Hitchiner

IPC: C12Q1/68 ,  C12N15/10 ,  B01L3/00 ,  B01L7/00 ,  G01F23/292 ,  C12N1/06 ,  B01L9/02 ,  G01N35/00 ,  C12Q1/6844

Abstract: A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements; a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including mRNA capture, cDNA synthesis, protein-associated assays, and library preparation, for next generation sequencing.

公开(公告)号：US20210171939A1

公开(公告)日：2021-06-10

申请号：US17109704

申请日：2020-12-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Adam McCoy ,  Kalyan Handique ,  Jonathan Bell ,  Vishal Sharma ,  Sida Wang

IPC: C12N15/10 ,  C12Q1/6853 ,  C12Q1/686

Abstract: An embodiment of a composition for target material separation includes: a body and one or more molecules coupled to the body and structured for functionalization of the composition. In embodiments, each of the one or more molecules can include one or more of: a linker region; a polymerase chain reaction (PCR) segment or oligo binding region; one or more barcode region(s); a unique molecule identifier; a preparation-facilitating segment; an active segment; and a molecular scissor or cleavage region, wherein various regions can be coupled together in order to provide functionality to the composition. The invention(s) also cover manufacturing of the composition and various applications of use, in the context of target material capture (e.g., from single cells or other biological material).