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1.发明申请ENZYMES FOR THE TREATMENT OF LIGNOCELLULOSICS, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 审中-公开用于治疗细胞凋亡的酶，编码其核酸的酶及其制备和使用它们的方法公开(公告)号：US20140223602A1
公开(公告)日：2014-08-07
申请号：US14039968
申请日：2013-09-27
申请人： Cathy Chang , Justin T. Stege , Nahla M. Aboushadi , Gordana Djordjevic , Ellen G. Burke , Peter Luginbuhl , Mark Dycaico , Toby H. Richardson , John Poland , Hefner Ying , Stacy M. Miles
发明人： Cathy Chang , Justin T. Stege , Nahla M. Aboushadi , Gordana Djordjevic , Ellen G. Burke , Peter Luginbuhl , Mark Dycaico , Toby H. Richardson , John Poland , Hefner Ying , Stacy M. Miles
CPC分类号： C12P19/14 , C12N9/2437 , C12N9/2445 , C12P7/04 , C12P19/02 , C12Y302/01021 , Y02E50/16 , Y02E50/17 , Y02E50/343
摘要： The invention provides polypeptides having a lignocellulolytic activity, e.g., a glycosyl hydrolase, a cellulase, an endoglucanase, a cellobiohydrolase, a beta-glucosidase, a xylanase, a mannanse, a xylosidase (e.g., a β-xylosidase), an arabinofuranosidase, and/or a glucose oxidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention provides polypeptides that can enzymatically process (hydrolyze) sugarcane bagasse, i.e., for sugarcane bagasse degradation, or for biomass processing, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. In one embodiment, the invention provides thermostable and thermotolerant forms of polypeptides of the invention.
摘要翻译： 本发明提供具有木质纤维素分解活性的多肽，例如糖基水解酶，纤维素酶，内切葡聚糖酶，纤维二糖水解酶，β-葡糖苷酶，木聚糖酶，甘露聚糖酶，木糖苷酶（例如，α-氧化酶），阿拉伯呋喃糖苷酶， 和/或葡萄糖氧化酶活性，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 一方面，本发明提供了可以酶促加工（水解）甘蔗渣，即甘蔗渣降解或用于生物量加工的多肽，以及编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和多肽。 在一个实施方案中，本发明提供本发明的多肽的耐热性和耐热性形式。
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公开(公告)号：US20120030840A1
公开(公告)日：2012-02-02
申请号：US13148294
申请日：2010-02-04
申请人： Stacy M. Miles
发明人： Stacy M. Miles
CPC分类号： C12N15/8246 , C12N9/2428 , C12N9/2437 , C12Y302/01091
摘要： Compositions and methods for expressing a multidomain enzyme in a plant are provided. The compositions include plants, seeds, plant tissues, and plant parts expressing a modified multidomain enzyme enzyme. The modified multidomain enzyme has a heterologous linker region that is not cleaved when the modified multidomain enzyme is expressed in a plant. In various embodiments, the linker region comprises the sequence set forth in SEQ ID NO:18, 19, or 20. Further provided are methods for producing a modified multidomain enzyme enzyme comprising cultivating plants expressing the modified multidomain enzyme. Downstream uses of transgenic plant material of the invention include agronomical and industrial uses, for example, human food, animal feed, pharmaceuticals, biofuel, industrial alcohol, fermentation feedstocks, and the like.
摘要翻译： 提供了在植物中表达多结构域酶的组合物和方法。 组合物包括植物，种子，植物组织和表达修饰的多结构域酶的植物部分。 修饰的多结构域酶具有在植物中表达修饰的多结构域酶时不裂解的异源接头区域。 在各种实施方案中，接头区包含SEQ ID NO：18,19或20所示的序列。进一步提供了制备修饰的多结构域酶的方法，其包括培养表达修饰的多结构域酶的植物。 本发明的转基因植物材料的下游用途包括农业和工业用途，例如人类食物，动物饲料，药物，生物燃料，工业酒精，发酵原料等。
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3.发明申请Glucanases, Nucleic Acids Encoding Them and Methods for Making and Using Them 审中-公开葡聚糖酶，编码它们的核酸及其制备和使用方法公开(公告)号：US20110117067A1
公开(公告)日：2011-05-19
申请号：US12375146
申请日：2007-08-04
IPC分类号： A61K35/12 , C07H21/04 , C07H21/00 , C12N15/63 , C12N5/10 , C12N1/21 , C12N1/15 , C12N1/19 , A01H5/10 , A01H5/00 , A01H1/00 , A61K38/47 , C12P1/00 , C12P19/00 , C12P7/04 , C12P7/06 , C12P7/16 , A61P43/00 , C09K8/02 , C09K8/60
CPC分类号： C12N9/2437 , A23K20/147 , A23L33/18 , C09K8/035 , C09K8/52 , C09K8/68 , C09K8/74 , C09K8/805 , C09K8/90 , C09K2208/24 , C12N9/2405 , C12P19/02 , C12P19/14 , C12Y302/01004 , C12Y302/01006 , C12Y302/01008 , C12Y302/01039 , E21B43/26 , G16B30/00 , Y02E50/17
摘要： The invention relates to polypeptides having glucanase, e.g., endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (e.g., endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (e.g., carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4 bonds in mixed beta-1,3 glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases e.g., endoglucanases, mannanases, xylanases have increased activity and stability, including thermotolerance or thermostability, at increased or decreased pHs and temperatures.
摘要翻译： 本发明涉及具有葡聚糖酶的多肽，例如内切葡聚糖酶，甘露聚糖酶，木聚糖酶活性或这些活性的组合，以及编码它们的多核苷酸。 一方面，葡聚糖酶活性是内切葡聚糖酶活性（例如内切-1,4-β-D-葡聚糖4-葡聚糖水解酶活性），并且包括在纤维素，纤维素衍生物中水解1,4-β-D-糖苷键 （例如，羧甲基纤维素和羟乙基纤维素）地衣淀粉，混合的β-1,3葡聚糖中的β-1,4键，例如谷类β-D-葡聚糖或木葡聚糖和含有纤维素部分的其它植物材料。 此外，还提供了设计新酶的方法及其使用方法。 在替代方面，新葡聚糖酶例如内切葡聚糖酶，甘露聚糖酶，木聚糖酶在增加或降低的pH和温度下具有增加的活性和稳定性，包括耐热性或热稳定性。
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公开(公告)号：US09012719B2
公开(公告)日：2015-04-21
申请号：US13148294
申请日：2010-02-04
申请人： Stacy M. Miles
发明人： Stacy M. Miles
CPC分类号： C12N15/8246 , C12N9/2428 , C12N9/2437 , C12Y302/01091
摘要： Compositions and methods for expressing a multidomain enzyme in a plant are provided. The compositions include plants, seeds, plant tissues, and plant parts expressing a modified multidomain enzyme enzyme. The modified multidomain enzyme has a heterologous linker region that is not cleaved when the modified multidomain enzyme is expressed in a plant. In various embodiments, the linker region comprises the sequence set forth in SEQ ID NO:18, 19, or 20. Further provided are methods for producing a modified multidomain enzyme enzyme comprising cultivating plants expressing the modified multidomain enzyme. Downstream uses of transgenic plant material of the invention include agronomical and industrial uses, for example, human food, animal feed, pharmaceuticals, biofuel, industrial alcohol, fermentation feedstocks, and the like.
摘要翻译： 提供了在植物中表达多结构域酶的组合物和方法。 组合物包括植物，种子，植物组织和表达修饰的多结构域酶的植物部分。 修饰的多结构域酶具有在植物中表达修饰的多结构域酶时不裂解的异源接头区域。 在各种实施方案中，接头区包含SEQ ID NO：18,19或20所示的序列。进一步提供了制备修饰的多结构域酶的方法，其包括培养表达修饰的多结构域酶的植物。 本发明的转基因植物材料的下游用途包括农业和工业用途，例如人类食物，动物饲料，药物，生物燃料，工业酒精，发酵原料等。
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