公开(公告)号：US10131958B1

公开(公告)日：2018-11-20

申请号：US16038887

申请日：2018-07-18

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P. A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68 ,  C12Q1/6888 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6874

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US20180327867A1

公开(公告)日：2018-11-15

申请号：US16038979

申请日：2018-07-18

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P.A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/6888 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6874

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2563/159 ,  C12Q2563/185

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US20180258500A1

公开(公告)日：2018-09-13

申请号：US15987851

申请日：2018-05-23

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P.A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2563/159 ,  C12Q2563/185

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US09905005B2

公开(公告)日：2018-02-27

申请号：US15409355

申请日：2017-01-18

Applicant: Cellular Research, Inc.

Inventor： Glenn Fu ,  Roger Rudoff ,  David Stern ,  George T. Wu ,  Stephen P. A. Fodor ,  Geoffrey Facer

IPC: G06K9/00 ,  G06T7/00 ,  G01N21/64 ,  G06K9/62

CPC classification number: G06T7/0012 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/6439 ,  G01N2201/129 ,  G06K9/6267 ,  G06T7/277 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/30072 ,  G06T2207/30242

Abstract: Methods, systems and platforms for digital imaging of multiple regions of an array, and detection and counting of the labeled features thereon, are described.

公开(公告)号：US20170314067A1

公开(公告)日：2017-11-02

申请号：US15581914

申请日：2017-04-28

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum ,  Glenn Fu

IPC: C12Q1/68

Abstract: In accordance with some embodiments herein, compositions and methods for accurate barcoding of nucleic acids are described. The compositions and methods can involve a plurality of unique oligonucleotide species comprising unique molecule barcodes. In some embodiments, the molecule barcodes can have a relatively low G content, and can exhibit reduced bias in amplification and analysis.

公开(公告)号：US09727810B2

公开(公告)日：2017-08-08

申请号：US15055445

申请日：2016-02-26

Applicant: Cellular Research, Inc.

Inventor： Stephen P. A. Fodor ,  Christina Fan ,  Glenn Fu ,  Geoffrey Facer

IPC: G06K19/06 ,  C12Q1/68

CPC classification number: G06K19/06103 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q2525/179 ,  C12Q2543/10 ,  C12Q2563/155

Abstract: The disclosure provides for methods, compositions, systems, devices, and kits for determining the number of distinct targets in distinct spatial locations within a sample. In some examples, the methods include: stochastically barcoding the plurality of targets in the sample using a plurality of stochastic barcodes, wherein each of the plurality of stochastic barcodes comprises a spatial label and a molecular label; estimating the number of each of the plurality of targets using the molecular label; and identifying the spatial location of each of the plurality of targets using the spatial label. The method can be multiplexed.

公开(公告)号：US11535882B2

公开(公告)日：2022-12-27

申请号：US15084307

申请日：2016-03-29

Applicant: Cellular Research, Inc.

Inventor： Glenn Fu ,  Stephen P. A. Fodor

IPC: C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6869

Abstract: The present disclosure provide compositions, methods and kits for generating a set of combinatorial barcodes, and uses thereof for barcoding samples such as single cells or genomic DNA fragments. Some embodiments disclosed herein provide compositions comprising a set of component barcodes for producing a set of combinatorial barcodes. The set of component barcodes can comprise, for example, n×m unique component barcodes, wherein n and m are integers, each of the component barcodes comprises: one of n unique barcode subunit sequences; and one or two linker sequences or the complements thereof, wherein the component barcodes are configured to connect to each other through the one or two linker sequences or the complements thereof to produce a set of combinatorial barcodes.

公开(公告)号：US10822643B2

公开(公告)日：2020-11-03

申请号：US15581914

申请日：2017-04-28

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum ,  Glenn Fu

IPC: C12Q1/6837 ,  C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/6874

Abstract: In accordance with some embodiments herein, compositions and methods for accurate barcoding of nucleic acids are described. The compositions and methods can involve a plurality of unique oligonucleotide species comprising unique molecule barcodes. In some embodiments, the molecule barcodes can have a relatively low G content, and can exhibit reduced bias in amplification and analysis.

公开(公告)号：US20190292592A1

公开(公告)日：2019-09-26

申请号：US16374626

申请日：2019-04-03

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum ,  Glenn Fu ,  Craig Betts

IPC: C12Q1/6874 ,  G16B25/00 ,  C12N15/10 ,  C12Q1/6806

Abstract: This disclosure provides methods and compositions for removing one or more high abundance species from a plurality of nucleic acid molecules. In some embodiments, the methods and compositions can be used for normalizing nucleic acid libraries. In some embodiments, molecular labels are used in conjunction with the methods and compositions disclosed herein to improve sequencing efficiency.

公开(公告)号：US20180037942A1

公开(公告)日：2018-02-08

申请号：US15665906

申请日：2017-08-01

Applicant: Cellular Research, Inc.

Inventor： Glenn Fu

IPC: C12Q1/68

CPC classification number: C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/6874 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2537/143 ,  C12Q2565/514

Abstract: The present disclosure relates to compositions, methods and kits for quantitative analysis of a plurality of nucleic acid target molecules in a sample. In some embodiments, the methods comprise associating molecular label sequences with target nucleic acid molecules without an enzymatic reaction.