公开(公告)号：US5204265A

公开(公告)日：1993-04-20

申请号：US933884

申请日：1992-08-21

申请人： Alan Nelson ,  Henry K. Hui ,  Monte Bennett ,  Soonkap Hahn ,  Charles S. Bankert

发明人： Alan Nelson ,  Henry K. Hui ,  Monte Bennett ,  Soonkap Hahn ,  Charles S. Bankert

IPC分类号： A61B5/00 ,  G01N21/77 ,  G01N33/00

CPC分类号： G01N21/7703 ,  A61B5/1455 ,  A61B5/1495 ,  G01N33/0006 ,  G01N2201/127 ,  Y10T436/10 ,  Y10T436/102499

摘要： The method of stabilizing a carbon dioxide sensor involves treatment of the sensor to reduce the instability that may occur in carbon dioxide sensors when such sensors are exposed to either very low or very high carbon dioxide levels for extended periods of time. This is accomplished by storing the sensor in an aqueous solution containing at least 2 weight percent carbon dioxide, for from several days to several months. The solution may be prepared in advance, or may be dynamically infused with carbon dioxide to provide the desired carbon dioxide content.

摘要翻译： 稳定二氧化碳传感器的方法包括处理传感器以减少在二氧化碳传感器中长时间暴露于非常低或非常高的二氧化碳水平时可能发生的不稳定性。 这通过将传感器储存在含有至少2重量％二氧化碳的水溶液中来实现，从几天到几个月。 该溶液可以预先制备，或者可以动态地注入二氧化碳以提供所需的二氧化碳含量。

公开(公告)号：US5378432A

公开(公告)日：1995-01-03

申请号：US132794

申请日：1993-10-07

申请人： Charles S. Bankert ,  Soonkap Hahn ,  Henry K. Hui

发明人： Charles S. Bankert ,  Soonkap Hahn ,  Henry K. Hui

IPC分类号： A61B5/145 ,  A61B5/00 ,  A61B5/1459 ,  G01N21/77 ,  G01N21/78 ,  G01N21/80 ,  G01N31/22 ,  G01N21/00 ,  C09B69/10

CPC分类号： A61B5/14539 ,  A61B5/1459 ,  G01N21/7703 ,  G01N31/221 ,  G01N2021/6434 ,  G01N2021/773 ,  G01N21/80

摘要： The optical fiber pH microsensor includes an optical fiber having a portion of the surface of a light conducting core covered with a layer of a pH sensitive dye material. The dye material is covalently bonded to a polymeric matrix which is in turn covalently bonded to the optical fiber core to prevent leaching of the indicator dye material during extended use. The dye material is crosslinked in situ over the tip of the optical fiber to yield a hydrophilic, ion permeable pH sensor which can be used intravascularly to monitor blood

摘要翻译： 光纤pH微传感器包括具有被一层pH敏感染料材料覆盖的导光核心表面的一部分的光纤。 染料材料共价键合到聚合物基质上，聚合物基体又与共聚键合到光纤芯上，以防止在延长使用期间浸出指示剂染料。 染料材料在光纤的尖端上原位交联，得到亲水的，离子可渗透的pH传感器，可用于血管内监测血液

公开(公告)号：US5277872A

公开(公告)日：1994-01-11

申请号：US902402

申请日：1992-06-19

申请人： Charles S. Bankert ,  Soonkap Hahn ,  Henry K. Hui

发明人： Charles S. Bankert ,  Soonkap Hahn ,  Henry K. Hui

IPC分类号： A61B5/145 ,  A61B5/00 ,  A61B5/1459 ,  G01N21/77 ,  G01N21/78 ,  G01N21/80 ,  G01N31/22 ,  G01N21/00 ,  C09B69/10

CPC分类号： A61B5/14539 ,  A61B5/1459 ,  G01N21/7703 ,  G01N31/221 ,  G01N2021/6434 ,  G01N2021/773 ,  G01N21/80

摘要： The optical fiber pH microsensor includes an optical fiber having a portion of the surface of a light conducting core covered with a layer of a pH sensitive dye material. The dye material is covalently bonded to a polymeric matrix which is in turn covalently bonded to the optical fiber core to prevent leaching of the indicator dye material during extended use. The dye material is crosslinked in situ over the tip of the optical fiber to yield a hydrophilic, ion permeable pH sensor which can be used intravascularly to monitor blood pH.

摘要翻译： 光纤pH微传感器包括具有被一层pH敏感染料材料覆盖的导光核心表面的一部分的光纤。 染料材料共价键合到聚合物基质上，聚合物基体又与共聚键合到光纤芯上，以防止在延长使用期间浸出指示剂染料。 染料材料在光纤的尖端上原位交联，得到亲水的离子可渗透的pH传感器，其可以血管内使用来监测血液pH。

公开(公告)号：US5246859A

公开(公告)日：1993-09-21

申请号：US888550

申请日：1992-05-22

申请人： Alan Nelson ,  Henry K. Hui ,  Monte Bennett ,  Soonkap Hahn ,  Charles S. Bankert ,  Jeffrey T. Jackson

发明人： Alan Nelson ,  Henry K. Hui ,  Monte Bennett ,  Soonkap Hahn ,  Charles S. Bankert ,  Jeffrey T. Jackson

IPC分类号： A61B5/145 ,  A61B5/1459 ,  G01N31/22 ,  G01N33/49 ,  G01N33/84

CPC分类号： G01N33/84 ,  G01N31/223 ,  Y10T436/102499 ,  Y10T436/108331 ,  Y10T436/20 ,  Y10T436/204998 ,  Y10T436/255

摘要： The method of stabilizing a carbon dioxide sensor involves formulation of a bicarbonate buffer solution in the sensor with a bicarbonate ion concentration of from about 100 mM to about 200 mM bicarbonate. The method also involves treatment of the sensor to reduce the instability that may occur in carbon dioxide sensors when such sensors are exposed to either very low or very high carbon dioxide levels for extended periods of time. The sensor is treated by exposing the sensor to an aqueous solution containing at least 2 weight percent carbon dioxide, for from several days to several months. The solution may be prepared in advance, or may be dynamically infused with carbon dioxide to provide the desired carbon dioxide content.

摘要翻译： 稳定二氧化碳传感器的方法包括在传感器中配制碳酸氢盐缓冲溶液，碳酸氢根离子浓度为约100mM至约200mM碳酸氢盐。 该方法还涉及传感器的处理，以减少二氧化碳传感器当这种传感器长时间暴露于非常低或非常高的二氧化碳水平时可能发生的不稳定性。 通过将传感器暴露于含有至少2重量％二氧化碳的水溶液中来处理传感器，持续数天至数月。 该溶液可以预先制备，或者可以动态地注入二氧化碳以提供所需的二氧化碳含量。

公开(公告)号：US5326531A

公开(公告)日：1994-07-05

申请号：US989102

申请日：1992-12-11

申请人： Soonkap Hahn ,  Alan Nelson ,  Monte Bennett ,  Henry K. Hui

发明人： Soonkap Hahn ,  Alan Nelson ,  Monte Bennett ,  Henry K. Hui

IPC分类号： A61B5/00 ,  G01N21/64 ,  G01N21/77 ,  G01N21/00 ,  G01N33/50

CPC分类号： G01N21/643 ,  A61B5/1455 ,  G01N21/7703 ,  G01N2021/772 ,  G01N2021/7786

摘要： The carbon dioxide sensor includes a CO.sub.2 sensing matrix formed from a hydrophilic, gas permeable, cross-linked polyurethane. The polyurethane matrix is formed from a polyurethane pre-polymer mixture which cross links when mixed with an aqueous carbon dioxide buffer solution. The dye-buffer solution is physically entrapped as a homogeneous dispersion in the polymer matrix. The matrix is applied to the exposed glass core of an optical fiber, which may first be coated with a primer to allow the polyurethane matrix to bond firmly to the tip of the optical fiber. The hydrophilic polymeric matrix is preferably coated with a hydrophobic material to help avoid leaching of the dye from the matrix, and to prevent diffusion of hydronium ions into the sensing matrix. The hydrophobic polymer overcoat may thereafter be cured by heat treatment. The hydrophobic coating may also contain a light reflective material such as TiO.sub.2.

摘要翻译： 二氧化碳传感器包括由亲水的，透气的交联聚氨酯形成的CO 2感测基质。 聚氨酯基质由与二氧化碳水溶液溶液混合时交联的聚氨酯预聚物混合物形成。 染料缓冲溶液在聚合物基质中物理截留为均匀分散体。 将基质施加到光纤的暴露的玻璃芯上，其可以首先用底漆涂布，以使聚氨酯基质牢固地结合到光纤的末端。 亲水性聚合物基质优选用疏水材料涂覆以帮助避免染料从基质中浸出，并防止水合氢离子扩散到感测基质中。 此后，疏水聚合物外涂层可以通过热处理固化。 疏水涂层还可以含有诸如TiO 2的光反射材料。

公开(公告)号：US07468249B2

公开(公告)日：2008-12-23

申请号：US10840208

申请日：2004-05-05

申请人： Zhiyi Xie ,  Soonkap Hahn ,  Tim Watanaskul

发明人： Zhiyi Xie ,  Soonkap Hahn ,  Tim Watanaskul

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6837 ,  C12Q2537/143 ,  C12Q2531/113

摘要： Methods for detecting in a single assay any one of multiple chromosomal disorders that result from aneuploidy or certain mutations, particularly microdeletions, and kits for use therein. A polymerase chain reaction (PCR) is carried out to amplify eukaryotic genomic DNA using a plurality of primer oligonucleotide pairs wherein one primer of each pair has a detectable label attached 5′ thereto. A plurality of the primer pairs are targeted to DNA segments of different chromosomes of interest which are indicative of potential chromosomal disorders, and one pair is targeted for a control gene. The amplified PCR products are purified, and single-stranded DNA having the detectable labels is obtained therefrom and hybridized with spots on a microarray that each contain DNA oligonucleotide probes having nucleotide sequences complementary to a nucleotide sequence of one strand of each segment. The microarray is imaged for presence of labels on its respective spots, and the absence or presence of chromosomal disorders as indicated by one or more of the targeted DNA segments of interest is diagnosed by first comparing the imaging results to the imaging of spots specific to the control gene and then to results obtained from imaging normal DNA.

摘要翻译： 在单次测定中检测由非整倍体或某些突变，特别是微缺失引起的多种染色体紊乱中的任何一种以及用于其中的试剂盒的方法。 进行聚合酶链反应（PCR）以使用多个引物寡核苷酸对扩增真核基因组DNA，其中每对的一个引物具有附着5'的可检测标记。 多个引物对靶向不同染色体的DNA片段，其指示潜在的染色体病症，一对靶向对照基因。 纯化扩增的PCR产物，从其中获得具有可检测标记的单链DNA，并与微阵列上的斑点杂交，每个点均含有具有与每个片段的一条链的核苷酸序列互补的核苷酸序列的DNA寡核苷酸探针。 成像微阵列以在其各自的斑点上存在标记，并且通过首先将成像结果与成像特异性的斑点的成像进行比较来诊断由一个或多个目标DNA片段指示的染色体病症的不存在或存在 控制基因，然后从成像正常DNA获得的结果。

公开(公告)号：US20050191636A1

公开(公告)日：2005-09-01

申请号：US10791209

申请日：2004-03-01

申请人： Soonkap Hahn

发明人： Soonkap Hahn

IPC分类号： C12Q1/68 ,  G01N33/48 ,  G01N33/50 ,  G06F19/00

CPC分类号： C12Q1/6883 ,  C12Q2600/156

摘要： Methods for detecting a short tandem repeat polymorphism (STRP), such as fragile X syndrome, wherein PCR is used to amplify nucleic acid along the chromosome in the genomic DNA which includes all of the STRs of interest plus a substantial contiguous segment of the nucleic acid adjacent to the STRs. Single-stranded product is then obtained, and colorimetric-labeled oligonucleotides which target for (i) STRs and (ii) the contiguous DNA segment are hybridized with this single-stranded product which is then bound to a solid phase and separated from the remainder of the target material. The labeled oligonucleotide target material is recovered by treatment with base and then hybridized to a microarray having a plurality of spots containing suitable oligonucleotide probes complementary thereto. Following hybridization, colorimetric intensities of the hybridized labeled target material present at specific spots on the microarray are measured to obtain individual values which are compared with results from known control samples to accurately quantify the number of STRs in the region of interest of the DNA being analyzed.

摘要翻译： 用于检测短串联重复多态性（STRP）的方法，例如脆性X综合征，其中使用PCR来扩增基因组DNA中沿着染色体的核酸，其包括所有感兴趣的STR加上核酸的相当连续的片段 毗邻STR。 然后获得单链产物，将靶向（i）STR和（ii）连续DNA片段的比色标记的寡核苷酸与该单链产物杂交，然后将其与固相结合并与其余部分分离 目标材料。 通过用碱处理回收标记的寡核苷酸靶物质，然后与具有多个含有与其互补的寡核苷酸探针的斑点的微阵列杂交。 杂交后，测量存在于微阵列特定斑点上的杂交标记的靶物质的比色强度，以获得与已知对照样品的结果进行比较以准确定量所分析的DNA感兴趣区域中STR的数目的个体值 。

公开(公告)号：US5645563A

公开(公告)日：1997-07-08

申请号：US377145

申请日：1995-01-24

申请人： Soonkap Hahn ,  John Merritt

发明人： Soonkap Hahn ,  John Merritt

IPC分类号： A61B5/00 ,  A61B17/135 ,  A61B17/42

CPC分类号： A61B17/42 ,  A61B17/1355

摘要： The childbirth-assisting device uses an automatically synchronized expandable pneumatic girdle to externally augment the secondary force of labor. The girdle through which the pressure is applied is fitted around the abdomen of the woman. An inflatable bladder within the girdle is inflated to create a downward pressure on the abdomen upon detection of a contraction. External pressure monitors which detect contractions may be attached directly to the girdle, and the girdle is configured to assure that the bladder is correctly positioned at all times. A member may be provided to prevent multiple uses of a girdle to assure the integrity of the girdle during use.

摘要翻译： 分娩辅助装置使用自动同步的可膨胀气动腰带来外部增加第二劳动力。 施加压力的腰带围绕着女人的腹部。 腰围内的充气气囊膨胀，以便在检测到收缩时在腹部产生向下的压力。 检测收缩的外部压力监视器可以直接附接到腰带，并且腰带被配置为确保膀胱在任何时候都被正确定位。 可以提供一个构件以防止腰带的多次使用以确保在使用过程中腰带的完整性。

公开(公告)号：US20050250111A1

公开(公告)日：2005-11-10

申请号：US10840208

申请日：2004-05-05

申请人： Zhiyi Xie ,  Soonkap Hahn ,  Tim Watanaskul

发明人： Zhiyi Xie ,  Soonkap Hahn ,  Tim Watanaskul

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6837 ,  C12Q2537/143 ,  C12Q2531/113

摘要： Methods for detecting in a single assay any one of multiple chromosomal disorders that result from aneuploidy or certain mutations, particularly microdeletions, and kits for use therein. A polymerase chain reaction (PCR) is carried out to amplify eukaryotic genomic DNA using a plurality of primer oligonucleotide pairs wherein one primer of each pair has a detectable label attached 5′ thereto. A plurality of the primer pairs are targeted to DNA segments of different chromosomes of interest which are indicative of potential chromosomal disorders, and one pair is targeted for a control gene. The amplified PCR products are purified, and single-stranded DNA having the detectable labels is obtained therefrom and hybridized with spots on a microarray that each contain DNA oligonucleotide probes having nucleotide sequences complementary to a nucleotide sequence of one strand of each segment. The microarray is imaged for presence of labels on its respective spots, and the absence or presence of chromosomal disorders as indicated by one or more of the targeted DNA segments of interest is diagnosed by first comparing the imaging results to the imaging of spots specific to the control gene and then to results obtained from imaging normal DNA.

摘要翻译： 在单次测定中检测由非整倍体或某些突变，特别是微缺失引起的多种染色体紊乱中的任何一种以及用于其中的试剂盒的方法。 进行聚合酶链反应（PCR）以使用多个引物寡核苷酸对扩增真核基因组DNA，其中每对的一个引物具有附着5'的可检测标记。 多个引物对靶向不同染色体的DNA片段，其指示潜在的染色体病症，一对靶向对照基因。 纯化扩增的PCR产物，从其中获得具有可检测标记的单链DNA，并与微阵列上的斑点杂交，每个点均含有具有与每个片段的一条链的核苷酸序列互补的核苷酸序列的DNA寡核苷酸探针。 成像微阵列以在其各自的斑点上存在标记，并且通过首先将成像结果与成像特异性的斑点的成像进行比较来诊断由一个或多个目标DNA片段指示的染色体病症的不存在或存在 控制基因，然后从成像正常DNA获得的结果。

公开(公告)号：US5871499A

公开(公告)日：1999-02-16

申请号：US842985

申请日：1997-04-25

申请人： Soonkap Hahn ,  John Merritt ,  Pat Roycroft ,  Steven T. Saville

发明人： Soonkap Hahn ,  John Merritt ,  Pat Roycroft ,  Steven T. Saville

IPC分类号： A61B17/135 ,  A61B17/42

CPC分类号： A61B17/42 ,  A61B17/1355

摘要： The childbirth-assisting device uses an automatically synchronized expandable pneumatic girdle to externally augment the secondary force of labor. The girdle through which the pressure is applied is fitted around the abdomen of the woman. An inflatable bladder within the girdle is inflated to create a downward pressure on the abdomen upon detection of a contraction. An external pressure monitor is used to detect contractions to provide a signal for synchronizing inflation of the girdle. The external pressure monitor includes a solid state force sensor embedded within a pliable housing which is conformable to the patient's abdomen to improve accuracy.

摘要翻译： 分娩辅助装置使用自动同步的可膨胀气动腰带来外部增加第二劳动力。 施加压力的腰带围绕着女人的腹部。 腰围内的充气气囊膨胀，以便在检测到收缩时在腹部产生向下的压力。 外部压力监视器用于检测收缩以提供用于同步腰带充气的信号。 外部压力监视器包括固定力传感器，其嵌入柔性外壳内，该固体力传感器符合患者的腹部以提高精度。