-
公开(公告)号:US20180073050A1
公开(公告)日:2018-03-15
申请号:US15702308
申请日:2017-09-12
Applicant: DSM IP ASSETS B.V
Inventor: Viktor Marius BOER , Nicolette Jasmijn Broers , Adam G. Lawrence
IPC: C12P19/56 , A23L2/60 , C07H15/256 , A23L27/30 , A23K20/105 , C12P15/00 , C12N15/52 , C12N9/90 , C12N9/88 , C12N9/10 , C12N9/02
CPC classification number: C12P19/56 , A23K20/105 , A23L2/60 , A23L27/36 , A23V2002/00 , C07H15/256 , C12N9/0042 , C12N9/0073 , C12N9/0083 , C12N9/1051 , C12N9/88 , C12N9/90 , C12N15/52 , C12P15/00 , C12Y114/13 , C12Y114/13078 , C12Y402/03019 , C12Y505/01013
Abstract: The present invention relates to a method for the production of a diterpene or a glycosylated diterpene, which method comprises: a. fermenting a recombinant microorganism in a suitable fermentation medium, wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol, whereby a diterpene or glycosylated diterpene is produced extracellularly in the fermentation medium; and b. recovering the diterpene or glycosylated diterpene from the fermentation medium.
-
公开(公告)号:US10913965B2
公开(公告)日:2021-02-09
申请号:US15774721
申请日:2016-11-14
Applicant: DSM IP ASSETS B.V. , Adam G. Lawrence , Celine ViArouge
Inventor: Adam G. Lawrence , Celine ViArouge
Abstract: The present disclosure relates to a novel method, expression vectors, and host cells for producing nicotinamide riboside by regulating the pathways that lead to the production of nicotinamide riboside.
-
公开(公告)号:US10273519B2
公开(公告)日:2019-04-30
申请号:US14906497
申请日:2014-07-23
Applicant: DSM IP ASSETS B.V.
Inventor: Nicolette Jasmijn Broers , Viktor Marius Boer , Adam G. Lawrence
IPC: C12P19/56 , A23L2/60 , C12N9/00 , C12N15/52 , C12N9/88 , C12N9/90 , C12N15/00 , C07C62/32 , C07H15/256 , C12N9/02 , C12N9/10 , C12P15/00 , A23L27/30
Abstract: The present invention relates to a method for the production of a diterpene or a glycosylated diterpene, which method comprises: a. fermenting a recombinant microorganism of the genus Yarrowia in a suitable fermentation medium at a temperature of about 29° C. or higher, wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol; and b. recovering the diterpene or glycosylated diterpene.
-
-