公开(公告)号：US20180073050A1

公开(公告)日：2018-03-15

申请号：US15702308

申请日：2017-09-12

Applicant: DSM IP ASSETS B.V

Inventor： Viktor Marius BOER ,  Nicolette Jasmijn Broers ,  Adam G. Lawrence

IPC: C12P19/56 ,  A23L2/60 ,  C07H15/256 ,  A23L27/30 ,  A23K20/105 ,  C12P15/00 ,  C12N15/52 ,  C12N9/90 ,  C12N9/88 ,  C12N9/10 ,  C12N9/02

CPC classification number: C12P19/56 ,  A23K20/105 ,  A23L2/60 ,  A23L27/36 ,  A23V2002/00 ,  C07H15/256 ,  C12N9/0042 ,  C12N9/0073 ,  C12N9/0083 ,  C12N9/1051 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12P15/00 ,  C12Y114/13 ,  C12Y114/13078 ,  C12Y402/03019 ,  C12Y505/01013

Abstract: The present invention relates to a method for the production of a diterpene or a glycosylated diterpene, which method comprises: a. fermenting a recombinant microorganism in a suitable fermentation medium, wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol, whereby a diterpene or glycosylated diterpene is produced extracellularly in the fermentation medium; and b. recovering the diterpene or glycosylated diterpene from the fermentation medium.

公开(公告)号：US10913965B2

公开(公告)日：2021-02-09

申请号：US15774721

申请日：2016-11-14

Applicant: DSM IP ASSETS B.V. ,  Adam G. Lawrence ,  Celine ViArouge

Inventor： Adam G. Lawrence ,  Celine ViArouge

IPC: C12P19/38 ,  C12N9/00 ,  C12N9/14 ,  A23L33/15

Abstract: The present disclosure relates to a novel method, expression vectors, and host cells for producing nicotinamide riboside by regulating the pathways that lead to the production of nicotinamide riboside.

公开(公告)号：US10273519B2

公开(公告)日：2019-04-30

申请号：US14906497

申请日：2014-07-23

Applicant: DSM IP ASSETS B.V.

Inventor： Nicolette Jasmijn Broers ,  Viktor Marius Boer ,  Adam G. Lawrence

IPC: C12P19/56 ,  A23L2/60 ,  C12N9/00 ,  C12N15/52 ,  C12N9/88 ,  C12N9/90 ,  C12N15/00 ,  C07C62/32 ,  C07H15/256 ,  C12N9/02 ,  C12N9/10 ,  C12P15/00 ,  A23L27/30

Abstract: The present invention relates to a method for the production of a diterpene or a glycosylated diterpene, which method comprises: a. fermenting a recombinant microorganism of the genus Yarrowia in a suitable fermentation medium at a temperature of about 29° C. or higher, wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol; and b. recovering the diterpene or glycosylated diterpene.