公开(公告)号：US20180073050A1

公开(公告)日：2018-03-15

申请号：US15702308

申请日：2017-09-12

Applicant: DSM IP ASSETS B.V

Inventor： Viktor Marius BOER ,  Nicolette Jasmijn Broers ,  Adam G. Lawrence

IPC: C12P19/56 ,  A23L2/60 ,  C07H15/256 ,  A23L27/30 ,  A23K20/105 ,  C12P15/00 ,  C12N15/52 ,  C12N9/90 ,  C12N9/88 ,  C12N9/10 ,  C12N9/02

CPC classification number: C12P19/56 ,  A23K20/105 ,  A23L2/60 ,  A23L27/36 ,  A23V2002/00 ,  C07H15/256 ,  C12N9/0042 ,  C12N9/0073 ,  C12N9/0083 ,  C12N9/1051 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12P15/00 ,  C12Y114/13 ,  C12Y114/13078 ,  C12Y402/03019 ,  C12Y505/01013

Abstract: The present invention relates to a method for the production of a diterpene or a glycosylated diterpene, which method comprises: a. fermenting a recombinant microorganism in a suitable fermentation medium, wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol, whereby a diterpene or glycosylated diterpene is produced extracellularly in the fermentation medium; and b. recovering the diterpene or glycosylated diterpene from the fermentation medium.

公开(公告)号：US10273519B2

公开(公告)日：2019-04-30

申请号：US14906497

申请日：2014-07-23

Applicant: DSM IP ASSETS B.V.

Inventor： Nicolette Jasmijn Broers ,  Viktor Marius Boer ,  Adam G. Lawrence

IPC: C12P19/56 ,  A23L2/60 ,  C12N9/00 ,  C12N15/52 ,  C12N9/88 ,  C12N9/90 ,  C12N15/00 ,  C07C62/32 ,  C07H15/256 ,  C12N9/02 ,  C12N9/10 ,  C12P15/00 ,  A23L27/30

Abstract: The present invention relates to a method for the production of a diterpene or a glycosylated diterpene, which method comprises: a. fermenting a recombinant microorganism of the genus Yarrowia in a suitable fermentation medium at a temperature of about 29° C. or higher, wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol; and b. recovering the diterpene or glycosylated diterpene.

公开(公告)号：US10260075B2

公开(公告)日：2019-04-16

申请号：US15407341

申请日：2017-01-17

Applicant: DSM IP ASSETS B.V.

Inventor： Paul Klaassen ,  Gijsberdina Pieternella Van Suylekom ,  Bianca Elisabeth Maria Gielesen ,  Nicolette Jasmijn Broers ,  Beate Wiedemann ,  Wilhelmus Theodorus Antonius Maria De Laat

IPC: C12P7/10 ,  C12P7/46

Abstract: The present invention relates to a process for the production of one or more fermentation product from a sugar composition, comprising the following steps: a) fermentation of the sugar composition in the presence of a yeast belonging to the genera Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces or Yarrowia, and b) recovery of the fermentation product, wherein the yeast comprises the genes araA, araB and araD and the sugar composition comprises glucose, galactose and arabinose.

公开(公告)号：US11649471B2

公开(公告)日：2023-05-16

申请号：US17199823

申请日：2021-03-12

Applicant: DSM IP ASSETS B.V.

Inventor： Paul Klaassen ,  Gijsberdina Pieternella Van Suylekom ,  Bianca Elisabeth Maria Gielesen ,  Nicolette Jasmijn Broers ,  Beate Wiedemann ,  Wilhelmus Theodorus Antonius Maria De Laat

IPC: C12P7/10 ,  C12P7/46 ,  C12N9/02 ,  C12N9/10 ,  C12N9/20 ,  C12N9/24 ,  C12N9/26 ,  C12N9/42 ,  C12N9/50 ,  C12N9/88

CPC classification number: C12P7/10 ,  C12P7/46 ,  Y02E50/10 ,  Y02E50/30

Abstract: The present invention relates to a process for the production of one or more fermentation product from a sugar composition, comprising the following steps:



a) fermentation of the sugar composition in the presence of a yeast belonging to the genera Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces or Yarrowia: and
b) recovery of the fermentation product,

wherein the yeast comprises the genes araA, araB and araD and the sugar composition comprises glucose, galactose and arabinose.

公开(公告)号：US10982235B2

公开(公告)日：2021-04-20

申请号：US16283208

申请日：2019-02-22

Applicant: DSM IP ASSETS B.V.

Inventor： Paul Klaassen ,  Gijsberdina Pieternella Van Suylekom ,  Bianca Elisabeth Maria Gielesen ,  Nicolette Jasmijn Broers ,  Beate Wiedemann ,  Wilhelmus Theodorus Antonius Maria De Laat

IPC: C12P7/10 ,  C12P7/46 ,  C12N9/02 ,  C12N9/10 ,  C12N9/20 ,  C12N9/24 ,  C12N9/26 ,  C12N9/42 ,  C12N9/50 ,  C12N9/88 ,  C12P5/02 ,  C12P7/04 ,  C12P7/16

Abstract: The present invention relates to a process for the production of one or more fermentation product from a sugar composition, comprising the following steps: a) fermentation of the sugar composition in the presence of a yeast belonging to the genera Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces or Yarrowia: and b) recovery of the fermentation product, wherein the yeast comprises the genes araA, araB and araD and the sugar composition comprises glucose, galactose and arabinose.