公开(公告)号：US09910041B2

公开(公告)日：2018-03-06

申请号：US14891727

申请日：2014-04-04

Applicant: EMD Millipore Corporation

Inventor： Matthew T. Stone ,  Ushma Mehta ,  Damon Asher ,  Patricia Greenhalgh

IPC: C07K16/00 ,  C07K1/36 ,  C07D471/02 ,  C07K1/16 ,  C07K1/18 ,  G01N33/569 ,  C07K1/20 ,  C07K1/22 ,  C12Q1/04 ,  C12Q1/06

CPC classification number: G01N33/56983 ,  C07K1/20 ,  C07K1/22 ,  C07K16/00 ,  C12Q1/04 ,  C12Q1/06 ,  G01N2333/015 ,  G01N2333/15

Abstract: The present invention provides methods for determining whether activated carbon can be used for removing viruses or a certain virus from a sample containing a target protein.

公开(公告)号：US20150166965A1

公开(公告)日：2015-06-18

申请号：US14382769

申请日：2013-03-13

Applicant: EMD Millipore Corporation

Inventor： Damon Asher ,  Anne Leahy

IPC: C12N7/00

CPC classification number: C12N7/00 ,  C12N2740/13051

Abstract: The invention relates to a process for increasing the observed titer of a virus stock for the purpose of increasing the calculated log reduction (LRV) in virus clearance studies. A tissue culture or assay plate is seeded with an indicator cell line and titrated with a virus stock followed, by a centrifugation step for about 5 minutes to about 24 hours at a g-force ranging from about 50×g to about 2400×g, and at a temperature from about 4° C. to about 39° C. The resulting calculated virus titer after undergoing the centrifugation step is 10-fold higher than the virus titer would be if determined in the absence of the centrifuging step.

Abstract translation: 本发明涉及增加病毒储存液观察滴度以增加病毒清除研究中计算的对数降低（LRV）的方法。 将组织培养物或测定板用指示细胞系接种，并用病毒原液滴定，然后通过离心步骤约50分钟至约2400×g的g-力进行约5分钟至约24小时， 并且在约4℃至约39℃的温度下进行。在经历离心步骤后得到的计算病毒滴度比不存在离心步骤时测定的病毒滴度高10倍。

公开(公告)号：US10066213B2

公开(公告)日：2018-09-04

申请号：US14382769

申请日：2013-03-13

Applicant: EMD Millipore Corporation

Inventor： Damon Asher ,  Anne Leahy

IPC: C12N15/867 ,  A61K48/00 ,  C07K14/005 ,  C12Q1/68 ,  A61K39/00 ,  C12N7/00

Abstract: The invention relates to a process for increasing the observed titer of a virus stock for the purpose of increasing the calculated log reduction (LRV) in virus clearance studies. A tissue culture or assay plate is seeded with an indicator cell line and titrated with a virus stock followed, by a centrifugation step for about 5 minutes to about 24 hours at a g-force ranging from about 50×g to about 2400×g, and at a temperature from about 4° C. to about 39° C. The resulting calculated virus titer after undergoing the centrifugation step is 10-fold higher than the virus titer would be if determined in the absence of the centrifuging step.