公开(公告)号：US20220267704A1

公开(公告)日：2022-08-25

申请号：US17742123

申请日：2022-05-11

Applicant: FUJIFILM Corporation

Inventor： Izumi OGURA ,  Keiji SHIGESADA ,  Shinji MIMA ,  Yuki IMAKURA ,  Shun GOTO ,  Nao YAMAZAKI

IPC: C12M1/04 ,  C12M1/00 ,  C12M1/12

Abstract: An object of the present invention is to provide a transportation instrument and a transportation method that enable transportation by using a cell culture container as it is. Further, an object of the present invention is to provide a transportation instrument and a transportation method that enable supply of oxygen required for respiration of cells. Further, an object of the present invention is to provide a transportation instrument and a transportation method that enable suppression of migration of a culture medium into and out of cells caused by the influence of an increase in internal pressure with respect to the temperature during transportation. According to the present invention, provided is a cell preservation or transportation instrument including a plurality of cell storage containers, a plate which has a plurality of recesses for holding the plurality of cell storage containers therein, and a flexible material sheet which seals upper opening portions of the plurality of cell storage containers and upper side wall portions formed by side walls of the recesses of the plate and allows ventilation between an inside and an outside of the plurality of cell storage containers.

公开(公告)号：US20250163381A1

公开(公告)日：2025-05-22

申请号：US19028665

申请日：2025-01-17

Applicant: FUJIFILM Corporation

Inventor： Izumi OGURA ,  Kentaro NAKAMURA

IPC: C12N5/0775

Abstract: In the research and cell therapy of a mesenchymal stem cell, it is necessary to secure a large number of cells. An object of the present invention is to provide a production method of a mesenchymal stem cell, which enables a large amount of mesenchymal stem cells maintaining undifferentiated properties to be produced. According to the present invention, there is provided a production method of a mesenchymal stem cell, including culturing a mesenchymal stem cell in a culture vessel in which a water contact angle of a bottom surface that is brought into contact with a cell is 70° or more, and subculturing the mesenchymal stem cell two or more times.

公开(公告)号：US20210147808A1

公开(公告)日：2021-05-20

申请号：US17158797

申请日：2021-01-26

Applicant: FUJIFILM Corporation

Inventor： Shinji MIMA ,  Yuki IMAKURA ,  Izumi OGURA

IPC: C12N5/071

Abstract: An object of the present invention is to provide a method for producing an intestinal epithelial cell in which the barrier function is maintained while the differentiation of a pluripotent stem cell into a liver cell is suppressed and an intestinal epithelial cell in which the barrier function is maintained while the differentiation into a liver cell is suppressed. According to the present invention, provided is the method for producing an intestinal epithelial cell, including a step 1 of differentiating a pluripotent stem cell into an intestinal stem cell and a step 2 of differentiating the intestinal stem cell obtained in the step 1 into an intestinal epithelial cell in a presence of one or more selected from the group consisting of a MEK1 inhibitor, a DNA methylation inhibitor, and a TGFβ receptor inhibitor, and EGF, in which during the step 2, a cell under differentiation is replated one or more times at the predetermined timing which is defined in the present specification.

公开(公告)号：US20230126568A1

公开(公告)日：2023-04-27

申请号：US18146198

申请日：2022-12-23

Applicant: FUJIFILM Corporation

Inventor： Yuki IMAKURA ,  Shinji MIMA ,  Izumi OGURA ,  Nao YAMAZAKI

IPC: C12N5/071

Abstract: An object of the present invention is to provide a method of producing an intestinal epithelial cell, which has a large number of cells per area and a high accuracy of kinetic prediction for a CYP3A4 substrate drug such as midazolam, by inducing the differentiation of a pluripotent stem cell, as well as the intestinal epithelial cell, a cell sheet, an evaluation method for a test substance, a screening kit for a test substance, and a cell preparation. According to the present invention, there is provided a production method for an intestinal epithelial cell, including a first differentiation step of differentiating a pluripotent stem cell into an intestinal stem cell, a proliferation step of proliferating the intestinal stem cell obtained in the differentiation step, and a second differentiation step of differentiating the intestinal stem cell obtained in the proliferation step into an intestinal epithelial cell, in which the proliferation step is a step of bringing the intestinal stem cell into a specific state.

公开(公告)号：US20140378662A1

公开(公告)日：2014-12-25

申请号：US14484838

申请日：2014-09-12

Applicant: FUJIFILM CORPORATION

Inventor： Shoji OYA ,  Hidekazu SAKAI ,  Ai KAWAKAMI ,  Takahiro HIRATSUKA ,  Hideo FUSHIMI ,  Kazuhiro YAMAGUCHI ,  Ichiro NISHIMURA ,  Yoshitaka OONO ,  Izumi OGURA

IPC: A61L27/22 ,  A61L27/54

CPC classification number: A61L27/222 ,  A61L27/52 ,  A61L27/54 ,  A61L2430/02 ,  A61L2430/34 ,  A61L2430/40

Abstract: A method of producing a tissue repair material, the method including: (a) obtaining a gelatin-containing intermediate that contains a gelatin and has a mesh structure, using a gelatin solution in which the gelatin is dissolved in an aqueous medium; (b) drying the gelatin-containing intermediate; and (c) cross-linking the gelatin before or after the drying of the gelatin-containing intermediate.

Abstract translation: 一种生产组织修复材料的方法，所述方法包括：（a）使用其中明胶溶于水性介质中的明胶溶液获得含有明胶并具有网状结构的含明胶的中间体; （b）干燥含明胶的中间体; 和（c）在含明胶的中间体的干燥之前或之后交联明胶。