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    DNA fragments containing biotin biosynthetase gene and use of the same
    1.
    发明授权
    DNA fragments containing biotin biosynthetase gene and use of the same 失效
    含有生物素生物合成酶基因的DNA片段及其用途

    公开(公告)号：US06410293B1

    公开(公告)日：2002-06-25

    申请号：US09180109

    申请日：1998-12-03

    申请人： Fujio Mukumoto ,  Shoichi Nishio ,  Jiro Akimaru ,  Satoshi Mitsuda

    发明人： Fujio Mukumoto ,  Shoichi Nishio ,  Jiro Akimaru ,  Satoshi Mitsuda

    IPC分类号： C12N910

    CPC分类号： C12N15/52 ,  C12P17/18

    摘要： A DNA fragment containing a gene concerned in biotin biosynthesis and derived from a microorganism belonging to the genus Sphingomonas, a plasmid containing said DNA fragment, and a biotin-producing transformant containing said plasmid. There is provided a technique for utilizing a gene concerned in biotin biosynthesis and derived from a microorganism belonging to the genus Sphingomonas, for breeding of a biotin-producing micro-organism by genetic engineering.

    摘要翻译： 包含生物素生物合成涉及基因，属于鞘氨醇单胞菌属微生物，含有该DNA片段的质粒和含有该质粒的生物素产生转化体的DNA片段。 提供了利用生物素生物合成相关基因来源于鞘氨醇单胞菌属微生物的技术，用于通过基因工程育种生物素生产微生物。

    Process for production of amide compounds using microorganism
    2.
    发明授权
    Process for production of amide compounds using microorganism 失效
    使用微生物生产酰胺化合物的方法

    公开(公告)号：US5563053A

    公开(公告)日：1996-10-08

    申请号：US382152

    申请日：1995-02-01

    申请人： Yoshiki Takashima ,  Fujio Mukumoto ,  Satoshi Mitsuda

    发明人： Yoshiki Takashima ,  Fujio Mukumoto ,  Satoshi Mitsuda

    IPC分类号： C12P13/02 ,  C12P17/12

    CPC分类号： C12R1/07 ,  C12P13/02

    摘要： The present invention provides a process for production of amide compounds, which includes converting a nitrile compound into its corresponding amide compound by hydrating the nitrile compound in the presence of a cultured broth of bacterial cells, intact bacterial cells, disrupted bacterial cells or enzymes contained therein, or immobilized preparations obtainable by immobilizing intact bacterial cells, disrupted bacterial cells or enzymes contained therein, the bacterial cells being those of a biologically pure culture of a thermophilic microorganism having hydration activity to convert a nitrile compound into its corresponding amide compound, such as Bacillus smithii SC-J05-1 (FERM BP-4935).

    摘要翻译： 本发明提供一种制备酰胺化合物的方法，其包括在培养的细菌细胞培养液，完整的细菌细胞，破坏的细菌细胞或其中含有的酶的存在下，使腈化合物水合，将腈化合物转化成其相应的酰胺化合物 或通过固定完整的细菌细胞，破坏的细菌细胞或其中所含的酶而获得的固定化制剂，细菌细胞是具有水合活性的嗜热微生物的生物学纯培养物的细菌细胞，其将腈化合物转化成其相应的酰胺化合物，例如芽孢杆菌 史密斯SC-J05-1（FERM BP-4935）。

    Method for improving optical purity of an amine compound
    5.
    发明授权
    Method for improving optical purity of an amine compound 失效
    提高胺化合物的光学纯度的方法

    公开(公告)号：US5965432A

    公开(公告)日：1999-10-12

    申请号：US990087

    申请日：1997-12-12

    申请人： Yuko Kobayashi ,  Satoshi Mitsuda ,  Ryohei Komaki

    发明人： Yuko Kobayashi ,  Satoshi Mitsuda ,  Ryohei Komaki

    IPC分类号： C12P13/00 ,  C12P41/00 ,  C12R1/06

    CPC分类号： C12P13/001 ,  C12P41/001 ,  Y10S435/83

    摘要： There is provided a method for improving optical purity of an amine compound of the formula I: ##STR1## wherein R.sup.1 indicates a phenyl group which may be substituted or an aralkyl group having 7 to 10 carbon atoms which may be substituted, and R.sup.2 indicates an alkyl group having 1 to 6 carbon atoms, which is characterized by contacting a cutlture of a microorganism belonging to Arthrobacter having an ability to preferentially metabolize one optical isomer based on an asymmetric carbon atom to which an amino group bonds in the amine compound of the formula I or a treated product thereof with the amine compound of the formula I.

    摘要翻译： 提供了提高式I的胺化合物的光学纯度的方法：其中R1表示可被取代的苯基或可被取代的具有7〜10个碳原子的芳烷基，R2表示具有 1至6个碳原子，其特征在于使属于节杆菌的微生物的切片与基于在式I的胺化合物中的氨基键合的不对称碳原子优先代谢一种光学异构体的能力接触，或 其处理产物与式I的胺化合物反应。

    Method for isolation of glucose isomerase
    6.
    发明授权
    Method for isolation of glucose isomerase 失效
    葡萄糖异构酶分离方法

    公开(公告)号：US4255521A

    公开(公告)日：1981-03-10

    申请号：US40872

    申请日：1979-05-21

    申请人： Hideo Hirohara ,  Shigeyasu Nabeshima ,  Satoshi Mitsuda ,  Tsuneyuki Nagase

    发明人： Hideo Hirohara ,  Shigeyasu Nabeshima ,  Satoshi Mitsuda ,  Tsuneyuki Nagase

    IPC分类号： C12N1/06 ,  C12N9/92

    CPC分类号： C12N9/92 ,  C12N1/066 ,  Y10S435/803 ,  Y10S435/886 ,  Y10S435/887

    摘要： In a process for extracting glucose isomerase from microorganism cells containing glucose isomerase by the so called high pressure release and impact method in which the suspension of the microorganism cells is homogenized for the extraction by releasing the pressure applied on said suspension instantaneously and giving the suspension an impact at very high velocity, the concentration of the suspension containing microorganism cells and the pressure applied on the said suspension are selected within specific ranges, namely from 0.5 to 10 wt. % (on a dry substance base) and from 400 to 700 Kg/cm.sup.2, respectively.

    摘要翻译： 在通过所谓的高压释放和冲击法从含有葡萄糖异构酶的微生物细胞中提取葡萄糖异构酶的方法中，其中微生物细胞的悬浮液通过释放立即施加在所述悬浮液上的压力并将悬浮液 以非常高的速度冲击，含有微生物细胞的悬浮液的浓度和施加在所述悬浮液上的压力在特定范围内选择，即0.5至10wt。 ％（在干物质基础上）和400至700Kg / cm 2。

    Esterase gene and its use
    9.
    发明授权
    Esterase gene and its use 失效
    酯酶基因及其用途

    公开(公告)号：US06306634B1

    公开(公告)日：2001-10-23

    申请号：US08979170

    申请日：1997-11-26

    申请人： Takeshi Ishii ,  Satoshi Mitsuda

    发明人： Takeshi Ishii ,  Satoshi Mitsuda

    IPC分类号： C12N918

    CPC分类号： C12P7/26 ,  C12N9/18 ,  C12P7/38 ,  C12P41/004

    摘要： An isolated esterase gene coding for an esterase capable of causing asymmetric hydrolysis of an organic carboxylic acid ester of a cyclopentenolone of formula I; wherein R1 is C1-C10 alkyl, C2-C10 alkenyl, C2-C10 alkynyl or C1-C4 haloalkyl, to produce the cyclopentenolone of formula I in (S)-form, and hybridizing to the base sequence of SEQ ID NO:1, is useful for the industrially favorable production of optically active cyclopentenolones of formula I which serve as the intermediates of drugs, agricultural chemicals or other active products.

    摘要翻译： 编码能够引起式I的环戊烯醇酮的有机羧酸酯的不对称水解的酯酶的分离的酯酶基因;其中R1是C1-C10烷基，C2-C10链烯基，C2-C10炔基或C1-C4卤代烷基， 产生（S） - 型的式I的环戊烯醇酮，并与SEQ ID NO：1的碱基序列杂交，可用于工业上有利地生产作为药物中间体的式I的光学活性的环戊烯醇酮，农药 或其他活性产品。