公开(公告)号：US20240229086A1

公开(公告)日：2024-07-11

申请号：US18284349

申请日：2022-03-22

Applicant: GENOMATICA, INC.

Inventor： Andreas W. SCHIRMER ,  Erin Frances PERRY ,  Alma Itzel RAMOS-SOLIS ,  Angelica ZABALA-BAUTISTA

IPC: C12P7/6409 ,  C12N1/20 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/16 ,  C12N9/88

CPC classification number: C12P7/6409 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/1288 ,  C12N9/16 ,  C12N9/88 ,  C12Y114/19002 ,  C12Y203/01084 ,  C12Y207/08007 ,  C12Y301/02014 ,  C12Y402/01059

Abstract: Recombinant proteobacteria, including γ-proteobacteria, comprising a heterologous acyl-ACP desaturase and a heterologous acyl-ACP thioesterase, wherein the native dual 3-hydroxy acyl-ACP dehydratase/isomerase is deleted are provided herein. The recombinant proteobacteria produce non-native monounsaturated free fatty acids or derivatives thereof. Methods of producing non-native monounsaturated free fatty acids or derivatives thereof are also provided, in addition to cell cultures and fatty acid compositions produced by the recombinant proteobacteria. The recombinant proteobacteria may be used to produce insect pheromones or precursors thereof, and fragrances or precursors thereof.