公开(公告)号：US12180515B2

公开(公告)日：2024-12-31

申请号：US17834681

申请日：2022-06-07

Applicant: Genomatica, Inc.

Inventor： Andreas Schirmer ,  Baolong Zhu ,  Emanuela Popova

IPC: C12N9/18 ,  C12N9/02 ,  C12N9/04 ,  C12N15/62 ,  C12N15/81

Abstract: The disclosure relates to omega-hydroxylase-related fusion polypeptides that result in improved omega-hydroxylated fatty acid derivative production when expressed in recombinant host cells. The disclosure further relates to microorganisms for expressing the omega-hydroxylase-related fusion polypeptides for the production of omega-hydroxylated fatty acid derivatives.

公开(公告)号：US20240336555A1

公开(公告)日：2024-10-10

申请号：US18294009

申请日：2022-09-19

Applicant: COVESTRO DEUTSCHLAND AG ,  Genomatica, Inc.

Inventor： Michael MERKEL

IPC: C07C211/12 ,  C07C263/10

CPC classification number: C07C211/12 ,  C07C263/10

Abstract: The invention relates to a method for the processing and transport of hexane-1,6-diamine or pentane-1,5-diamine comprising the steps of: a) Providing a first composition including 60 to 98 wt.-% water and 2 to 40 wt.-% hexane-1,6-diamine or pentane-1,5-diamine, b) partially removing water from the first composition by distillation at a first production location, whereby a second composition is generated, said second composition including the hexane-1,6-diamine or pentane-1,5-diamine and 1 wt.-% to 35 wt.-% water, and c) transporting said second composition during a span of time t from the first production location to a second production location, wherein the span of time t including times for optional temporary storage is at least 6 h.

公开(公告)号：US12098168B2

公开(公告)日：2024-09-24

申请号：US17271442

申请日：2019-08-29

Applicant: Genomatica, Inc.

Inventor： Stephen Thomas Payne ,  Scott Allen Frykman ,  Bernardo Moura Torres Da Costa ,  Isolde Callihan ,  Sankaranarayanan Venkiteswaran ,  Leland Ken Wong

IPC: C07K14/245 ,  C12N1/22 ,  C12N9/02 ,  C12N9/06 ,  C12N9/10 ,  C12N9/18 ,  C12P7/6436

CPC classification number: C07K14/245 ,  C12N1/22 ,  C12N9/0008 ,  C12N9/0014 ,  C12N9/0077 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/18 ,  C12P7/6436 ,  C12Y102/99006 ,  C12Y104/99003 ,  C12Y114/15003 ,  C12Y203/01075 ,  C12Y206/01001 ,  C12Y301/01067

Abstract: The disclosure relates to mutant gene(s) that confer upon microorganisms that express them an improved capacity to utilize xylose and improved capacity to co-utilize glucose and xylose thereby resulting in improved growth of the microorganism. Further encompassed are methods of producing fatty acids and fatty acid derivatives from cellulosic biomass, xylose, and/or a glucose/xylose mix by employing the host cells expressing the engineered XylR variants and compositions of biologically produced fatty acids and fatty acid derivatives.

公开(公告)号：US20240294950A1

公开(公告)日：2024-09-05

申请号：US18390570

申请日：2023-12-20

Applicant: Genomatica, Inc.

Inventor： Robin E. Osterhout ,  Anthony P. Burgard ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P5/02 ,  C12N1/14 ,  C12N1/20 ,  C12N15/70 ,  C12N15/81 ,  C12P7/02 ,  C12P7/04 ,  C12P7/18 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P13/00 ,  C12P17/10

CPC classification number: C12P5/026 ,  C12N1/14 ,  C12N1/20 ,  C12N15/70 ,  C12N15/81 ,  C12P7/02 ,  C12P7/04 ,  C12P7/18 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12P17/10 ,  Y02E50/30

Abstract: The invention provides non-naturally occurring microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing carbon flux through acetyl-CoA. In some embodiments, the microbial organisms of the invention having such pathways also include pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation. The enhanced carbon flux through acetyl-CoA, in combination with pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation can, in some embodiments, be used for production of a bioderived compound. Accordingly, in some embodiments, the microbial organisms of the invention can include a pathway capable of producing a bioderived compound of the invention. The invention still further provides a bioderived compound produced by a microbial organism of the invention, culture medium having the bioderived compound of the invention, compositions having the bioderived compound of the invention, a biobased product comprising the bioderived compound of the invention, and a process for producing a bioderived compound of the invention.

公开(公告)号：US20240240160A1

公开(公告)日：2024-07-18

申请号：US18564967

申请日：2022-05-27

Applicant: GENOMATICA, INC.

Inventor： Andreas W. SCHIRMER ,  Michael Angus NOBLE ,  Jamison Parker HUDDLESTON

IPC: C12N9/10 ,  C12N15/52 ,  C12N15/70 ,  C12P7/22 ,  C12P7/42 ,  C12P17/06 ,  C12P19/32

CPC classification number: C12N9/1029 ,  C12N15/52 ,  C12N15/70 ,  C12P7/22 ,  C12P7/42 ,  C12P17/06 ,  C12P19/32 ,  C12Y203/01206

Abstract: The present disclosure provides a poly nucleotide comprising: (a) a nucleic acid sequence encoding an olivetol synthase (OLS): and (b) a heterologous regulatory element operably linked to the nucleic acid sequence. The present disclosure further relates to an engineered cell comprising an olivetol synthase (OLS). Also provided are a cell extract or cell culture medium or a composition comprising 3.5.7- trioxododecanoyl-CoA, olivetol, olivetolic acid, a cannabinoid, and/or an isomer, analog, or derivative thereof, and a method of making 3.5.7-trioxododecanoyl-CoA, olivetol, olivetolic acid, a cannabinoid, and/or an isomer, analog, or derivative thereof. Also provided is a cannabinoid produced by the engineered cell, isolated from the cell extract or cell culture medium, and/or made by the method described herein. The present disclosure also provides a non-natural olivetol synthase (OLS).

公开(公告)号：US20240150774A1

公开(公告)日：2024-05-09

申请号：US18344219

申请日：2023-06-29

Applicant: Genomatica, Inc.

Inventor： Derek L. GREENFIELD ,  Andreas W. Schirmer ,  Elizabeth J. Clarke ,  Eli S. Groban ,  Bernardo M. Da Costa ,  Zhihao Hu ,  Kevin Holden ,  Noah Helman

IPC: C12N15/70 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N9/90 ,  C12P7/04 ,  C12P7/6409 ,  C12P7/6436

CPC classification number: C12N15/70 ,  C12N9/0006 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1288 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12P7/04 ,  C12P7/6409 ,  C12P7/6436 ,  C12Y101/011 ,  C12Y103/01009 ,  C12Y103/0101 ,  C12Y203/01039 ,  C12Y203/01041 ,  C12Y203/01179 ,  C12Y203/0118 ,  C12Y207/08007 ,  C12Y402/01059 ,  C12Y503/03014 ,  C12Y604/01002

Abstract: The disclosure relates to recombinant host cells including strain modifications effective to improve titer, yield and/or productivity of fatty acid derivatives. The disclosure further relates to cell cultures including the recombinant host cells for the fermentative production of fatty acid derivatives and compositions thereof.

公开(公告)号：US20240124904A1

公开(公告)日：2024-04-18

申请号：US18453987

申请日：2023-08-22

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Priti Pharkya ,  Tae Hoon Yang ,  Jungik Choi

IPC: C12P7/18 ,  C07K14/245 ,  C12N1/20 ,  C12N9/00 ,  C12N9/02 ,  C12N9/12 ,  C12N9/16 ,  C12N9/52 ,  C12N9/88 ,  C12N15/52 ,  C12P7/42

CPC classification number: C12P7/18 ,  C07K14/245 ,  C12N1/20 ,  C12N9/0036 ,  C12N9/0057 ,  C12N9/1205 ,  C12N9/16 ,  C12N9/52 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12P7/42 ,  C12Y106/01002 ,  C12Y110/03 ,  C12Y207/0104 ,  C12Y301/00 ,  C12Y301/02 ,  C12Y301/02002 ,  C12Y304/21092 ,  C12Y401/01031 ,  C12Y401/01032

Abstract: −The invention is directed to a non-naturally occurring microbial organism comprising a first attenuation of a succinyl-CoA synthetase or transferase and at least a second attenuation of a succinyl-CoA converting enzyme or a gene encoding a succinate producing enzyme within a multi-step pathway having a net conversion of succinyl-CoA to succinate.

公开(公告)号：US11898172B2

公开(公告)日：2024-02-13

申请号：US16498336

申请日：2018-03-29

Applicant: Genomatica, Inc.

Inventor： Kevin Hoff ,  Cara Ann Tracewell ,  Kui Chan ,  Michael Kuchinskas ,  Harish Nagarajan

IPC: C12N9/04 ,  C12N9/10 ,  C12P7/18 ,  C12P7/24 ,  C12P7/52 ,  C12P19/32

CPC classification number: C12N9/0006 ,  C12N9/1029 ,  C12P7/18 ,  C12P7/24 ,  C12P7/52 ,  C12P19/32 ,  C12Y101/01157 ,  C12Y203/01009

Abstract: The present disclosure provides thiolases and polypeptide variants of 3-hydroxybutyryl-CoA dehydrogenase, nucleic acids encoding the same, vectors comprising the nucleic acids, and cells comprising the polypeptide variants and/or thiolase, the nucleic acids, and/or the vectors. The present disclosure also provides methods of making and using the same, including methods for culturing cells, and for the production of various products, including 3-hydroxybutyryl-CoA (3-HB-CoA), 3-hydroxybutyraldehyde (3-HBal), 3-hydroxybutyrate (3-HB), 1,3-butanediol (1,3-BDO), and esters and amides thereof, and products made from any of these.

公开(公告)号：US20230374473A1

公开(公告)日：2023-11-23

申请号：US18316993

申请日：2023-05-12

Applicant: Genomatica, Inc.

Inventor： Michael A. Noble ,  Kevin G. Hoff ,  Anna Lechner ,  Harish Nagarajan

IPC: C12N9/10 ,  C12P7/22 ,  C12P7/42

CPC classification number: C12N9/1085 ,  C12P7/22 ,  C12P7/42

Abstract: Described herein are prenyltransferases including non-natural variants thereof having at least one amino acid substitution as compared to its corresponding natural or unmodified prenyltransferases and that are capable of at least two-fold greater rate of formation of cannabinoids such as cannabigerolic acid, cannabigerovarinic acid, cannabigerorcinic acid, and cannabigerol, as compared to a wild type control. Prenyltransferase variants also demonstrated regioselectivity to desired cannabinoid isomers such as CDBA (3-GOLA), 3-GDVA, 3-GOSA, and CBG (2-GOL). The prenyltransferase variants can be used to form prenylated aromatic compounds, and can be expressed in an engineered microbe having a pathway to such compounds, which include 3-GOLA, 3-GDVA, 3-GOSA, and CBG. 3-GOLA can be used for the preparation of cannabigerol (CBG), which can be used in therapeutic compositions.

公开(公告)号：US11753372B2

公开(公告)日：2023-09-12

申请号：US17418060

申请日：2019-12-23

Applicant: AQUAFIL S.P.A. ,  GENOMATICA INC.

Inventor： Michele Cecchetto ,  Anacleto Dal Moro ,  Lauri Hannunpoika Suominen ,  Michael Japs

IPC: C07D201/08

CPC classification number: C07D201/08

Abstract: A new process for the production of epsilon caprolactam (CPL) from 6-aminocaproic acid (6-ACA) can be obtained either from traditional petro chemical processes or can be obtained from biochemical processes. With the proposed process, the reaction time for conversion of 6-aminocaproic acid to the Nylon 6 monomer is shorter and significant energy savings are possible which is advantageous for industrial scale production. The conversion of 6-aminocaproic acid to the Nylon 6 monomer runs at atmospheric pressure and in the final product epsilon caprolactam with no oligomers formation of significance is obtained.