公开(公告)号：US20250101471A1

公开(公告)日：2025-03-27

申请号：US18800482

申请日：2024-08-12

Applicant: Genomatica, Inc.

Inventor： Andreas W. SCHIRMER ,  Noah HELMAN ,  Haibo WANG ,  Zhihao HU ,  Vikranth ARLAGADDA ,  Alma Itzel RAMOS-SOLIS ,  Elizabeth J. CLARKE

IPC: C12P7/18 ,  C12N9/00 ,  C12N9/16 ,  C12N15/52

Abstract: The disclosure relates to fatty diols and recombinant microorganisms for producing them. More particularly, the disclosure relates to recombinant microorganisms engineered to produce fatty diols via fermentation. Further encompassed is a process that uses the microorganisms to produce fatty diols from a simple carbon source.

公开(公告)号：US20220267813A1

公开(公告)日：2022-08-25

申请号：US17732799

申请日：2022-04-29

Applicant: Genomatica, Inc.

Inventor： Andreas W. SCHIRMER ,  Noah HELMAN ,  Haibo WANG ,  Zhihao HU ,  Vikranth ARLAGADDA ,  Alma Itzel RAMOS-SOLIS ,  Elizabeth CLARKE

IPC: C12P7/18 ,  C12N9/00 ,  C12N9/16 ,  C12N15/52

Abstract: The disclosure relates to fatty diols and recombinant microorganisms for producing them. More particularly, the disclosure relates to recombinant microorganisms engineered to produce fatty diols via fermentation. Further encompassed is a process that uses the microorganisms to produce fatty diols from a simple carbon source.

公开(公告)号：US20240240160A1

公开(公告)日：2024-07-18

申请号：US18564967

申请日：2022-05-27

Applicant: GENOMATICA, INC.

Inventor： Andreas W. SCHIRMER ,  Michael Angus NOBLE ,  Jamison Parker HUDDLESTON

IPC: C12N9/10 ,  C12N15/52 ,  C12N15/70 ,  C12P7/22 ,  C12P7/42 ,  C12P17/06 ,  C12P19/32

CPC classification number: C12N9/1029 ,  C12N15/52 ,  C12N15/70 ,  C12P7/22 ,  C12P7/42 ,  C12P17/06 ,  C12P19/32 ,  C12Y203/01206

Abstract: The present disclosure provides a poly nucleotide comprising: (a) a nucleic acid sequence encoding an olivetol synthase (OLS): and (b) a heterologous regulatory element operably linked to the nucleic acid sequence. The present disclosure further relates to an engineered cell comprising an olivetol synthase (OLS). Also provided are a cell extract or cell culture medium or a composition comprising 3.5.7- trioxododecanoyl-CoA, olivetol, olivetolic acid, a cannabinoid, and/or an isomer, analog, or derivative thereof, and a method of making 3.5.7-trioxododecanoyl-CoA, olivetol, olivetolic acid, a cannabinoid, and/or an isomer, analog, or derivative thereof. Also provided is a cannabinoid produced by the engineered cell, isolated from the cell extract or cell culture medium, and/or made by the method described herein. The present disclosure also provides a non-natural olivetol synthase (OLS).

公开(公告)号：US20210310033A1

公开(公告)日：2021-10-07

申请号：US17229543

申请日：2021-04-13

Applicant: Genomatica, Inc.

Inventor： Stephen B. DEL CARDAYRE ,  Andreas W. SCHIRMER ,  Myong KO ,  Haibo WANG

IPC: C12P17/08 ,  C12P17/04 ,  C12P7/44 ,  C12P7/42

Abstract: The present disclosure relates to processes that combine microbial production of organic intermediates and subsequent synthetic transformation to provide compounds of industrial value, including compounds used in fragrances.

公开(公告)号：US20230332193A1

公开(公告)日：2023-10-19

申请号：US18043258

申请日：2021-09-01

Applicant: GENOMATICA, INC.

Inventor： Andreas W. SCHIRMER ,  Deqiang ZHANG ,  Jamison Parker HUDDLESTON

IPC: C12P17/06 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N15/52

CPC classification number: C12P17/06 ,  C12N9/0006 ,  C12N9/1029 ,  C12Y203/01206 ,  C12N9/88 ,  C12Y404/01026 ,  C12N9/1085 ,  C12Y205/0101 ,  C12N15/52

Abstract: The disclosure relates to a non-natural flavin-dependent oxidase comprising at least one amino acid variation as compared to a wild type flavin-dependent oxidase, wherein the non-natural flavin-dependent oxidase does not comprise a disulfide bond, and wherein the non-natural flavin-dependent oxidase is capable of oxidative cyclization of a prenylated aromatic compound into a cannabinoid. The disclosure also relates to a nucleic acid, an expression construct, and an engineered cell for making the non-natural flavin-dependent oxidase. Also provided are compositions comprising the non-natural flavin-dependent oxidase; isolated non-natural flavin-dependent oxidase and methods of making the same; cell extracts comprising the non-natural flavin-dependent oxidase; and methods of making cannabinoids.

公开(公告)号：US20210348173A1

公开(公告)日：2021-11-11

申请号：US17335812

申请日：2021-06-01

Applicant: Genomatica, Inc.

Inventor： Derek L. GREENFIELD ,  Andreas W. SCHIRMER ,  Elizabeth J. CLARKE ,  Eli S. GROBAN ,  Bernardo M. DA COSTA ,  Zhihao HU ,  Kevin HOLDEN ,  Noah HELMAN

IPC: C12N15/70 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N9/90 ,  C12N9/00 ,  C12P7/04 ,  C12P7/64

Abstract: The disclosure relates to recombinant host cells including strain modifications effective to improve titer, yield and/or productivity of fatty acid derivatives. The disclosure further relates to cell cultures including the recombinant host cells for the fermentative production of fatty acid derivatives and compositions thereof.

公开(公告)号：US20210009983A1

公开(公告)日：2021-01-14

申请号：US16802133

申请日：2020-02-26

Applicant: Genomatica, Inc.

Inventor： Derek L. GREENFIELD ,  Donald E. TRIMBUR ,  Andreas W. SCHIRMER ,  Cindy CHANG ,  Behnaz BEHROUZIAN ,  Jessica WINGER

IPC: C12N9/00 ,  C07K14/245 ,  C12P7/64

Abstract: The disclosure relates to acetyl-CoA carboxylase (ACC) variants and host cells expressing them for the production of malonyl-CoA derived compounds including fatty acid derivatives. Further contemplated are methods of producing increased amounts of malonyl-CoA derived compounds and related cell cultures.

公开(公告)号：US20220064684A1

公开(公告)日：2022-03-03

申请号：US17420908

申请日：2020-01-15

Applicant: Genomatica, Inc.

Inventor： Andreas W. SCHIRMER ,  Katherine Ann MURPHY ,  Angelica Zabala BAUTISTA

IPC: C12P17/06 ,  C12N9/16 ,  C12N9/00

Abstract: The disclosure relates to methods for the production of natural lactones by bacteria under physiological conditions. The methods employ ybgC proteins having lactonizing activity.

公开(公告)号：US20210189439A1

公开(公告)日：2021-06-24

申请号：US17053190

申请日：2019-05-03

Applicant: Genomatica, Inc.

Inventor： Andreas W. SCHIRMER ,  Risha Lindig BOND ,  Erin Frances PERRY

IPC: C12P7/18 ,  C12N15/52

Abstract: The disclosure relates to the field of specialty chemicals and methods for their synthesis. In embodiments, the disclosure provides novel multifunctional fatty acid derivative molecules such as e.g., fatty triols, fatty tetrols, dihydroxy fatty acids, etc. The disclosure further provides derivatives of the disclosed multifunctional molecules which are useful e.g., in the production of personal care products, surfactants, detergents, polymers, paints, coatings, and as emulsifiers, emollients, and thickeners in cosmetics and foods, as industrial solvents and plasticizers, etc. The disclosure further provides biochemical pathways, recombinant microorganisms and methods for the biological production of various multifunctional fatty acid derivatives.

公开(公告)号：US20210189373A1

公开(公告)日：2021-06-24

申请号：US17271439

申请日：2019-08-28

Applicant: Genomatica, Inc.

Inventor： Andreas W. SCHIRMER ,  Katherine Ann MURPHY ,  Erin Frances PERRY

IPC: C12N9/88 ,  C12N9/16 ,  C12N9/10 ,  C12N9/02 ,  C12N9/04 ,  C12P7/64

Abstract: The disclosure relates to the field of specialty chemicals and methods for their synthesis. In embodiments, the disclosure provides viable bacterial cells which comprise heterologous dual 3-hydroxy-acyl-ACP dehydratase/isomerases, etc. The disclosure further provides monounsaturated fatty acid derivative molecules produced by the viable bacterial cells which are non-native to the bacterial cells. The disclosure further provides methods for the preparation and production of non-native monounsaturated fatty acid derivative molecules such as e.g., an ω3-monounsaturated fatty acid derivative, an ω5-monounsaturated fatty acid derivative, an ω9-monounsaturated fatty acid derivative, an ω11-monounsaturated fatty acid fatty acid derivative, etc.