公开(公告)号：US20200299402A1

公开(公告)日：2020-09-24

申请号：US16842864

申请日：2020-04-08

Applicant: GENZYME CORPORATION

Inventor： Kevin P. Brower ,  Chris Hwang ,  Rao Koduri ,  Konstantin B. Konstantinov ,  Veena Warikoo ,  Jin Yin ,  Marcella Yu

IPC: C07K16/28 ,  C07K16/00 ,  C07K1/113 ,  A61K39/395 ,  C07K16/22

Abstract: Disclosed herein are methods that have been developed to control the formation of disulfide bonds between polypeptides of a multimeric protein produced by a bioprocess. Also disclosed are protein solution parameters that allow for controlling the formation of disulfide bonds. In one example, the methods disclosed herein can be used to control the proportion of half antibody molecules in an antibody solution.

公开(公告)号：US20160108127A1

公开(公告)日：2016-04-21

申请号：US14668820

申请日：2015-03-25

Applicant: Genzyme Corporation

Inventor： Kevin P. Brower ,  Chris Hwang ,  Rao Koduri ,  Konstantin B. Konstantinov ,  Veena Warikoo ,  Jin Yin ,  Marcella Yu

IPC: C07K16/28 ,  C07K16/22

CPC classification number: C07K16/2896 ,  A61K39/39591 ,  C07K1/1133 ,  C07K16/00 ,  C07K16/22 ,  C07K16/2839 ,  C07K2317/30 ,  C07K2317/52

Abstract: Disclosed herein are methods that have been developed to control the formation of disulfide bonds between polypeptides of a multimeric protein produced by a bioprocess. Also disclosed are protein solution parameters that allow for controlling the formation of disulfide bonds. In one example, the methods disclosed herein can be used to control the proportion of half antibody molecules in an antibody solution.

Abstract translation: 本文公开了已经开发用于控制由生物过程产生的多聚体蛋白质的多肽之间形成二硫键的方法。 还公开了允许控制二硫键形成的蛋白质溶液参数。 在一个实例中，本文公开的方法可用于控制抗体溶液中半抗体分子的比例。

公开(公告)号：US20210269477A1

公开(公告)日：2021-09-02

申请号：US17321128

申请日：2021-05-14

Applicant: Genzyme Corporation

Inventor： Konstantin Konstantinov ,  Benjamin Wright ,  Jin Yin ,  Marcella Yu ,  Hang Zhou

IPC: C07K1/34 ,  C12M1/00 ,  C12M1/34 ,  C07K14/475 ,  C07K14/52 ,  C07K16/00 ,  C12M1/26 ,  C12N9/00

Abstract: Provided herein are methods of processing a cell culture and open circuit filtration systems.

公开(公告)号：US10647777B2

公开(公告)日：2020-05-12

申请号：US14668820

申请日：2015-03-25

Applicant: Genzyme Corporation

Inventor： Kevin P. Brower ,  Chris Hwang ,  Rao Koduri ,  Konstantin B. Konstantinov ,  Veena Warikoo ,  Marcella Yu ,  Jin Yin

IPC: C07K1/113 ,  A61K39/395 ,  C07K16/28 ,  C07K16/00 ,  C07K16/22

Abstract: Disclosed herein are methods that have been developed to control the formation of disulfide bonds between polypeptides of a multimeric protein produced by a bioprocess. Also disclosed are protein solution parameters that allow for controlling the formation of disulfide bonds. In one example, the methods disclosed herein can be used to control the proportion of half antibody molecules in an antibody solution.

公开(公告)号：US20150158907A1

公开(公告)日：2015-06-11

申请号：US14627674

申请日：2015-02-20

Applicant: Genzyme Corporation

Inventor： Hang Zhou ,  Benjamin Wright ,  Marcella Yu ,  Jin Yin ,  Konstantin Konstantinov

IPC: C07K1/34 ,  C07K14/52 ,  C12N9/00 ,  C07K14/475 ,  C12M1/26 ,  C07K16/00

CPC classification number: C07K1/34 ,  C07K14/475 ,  C07K14/52 ,  C07K16/00 ,  C12M29/00 ,  C12M29/04 ,  C12M29/10 ,  C12M29/18 ,  C12M33/14 ,  C12M41/00 ,  C12M47/10 ,  C12M47/12 ,  C12N9/00

Abstract: Provided herein are methods of processing a cell culture and open circuit filtration systems.

Abstract translation: 本文提供了处理细胞培养和开路过滤系统的方法。