公开(公告)号：US20230383004A1

公开(公告)日：2023-11-30

申请号：US18194471

申请日：2023-03-31

Applicant: Genentech, Inc.

Inventor： Yung-Hsiang KAO ,  Michael W. LAIRD ,  Melody Trexler SCHMIDT ,  Rita L. WONG ,  Daniel P. HEWITT

IPC: C07K16/28 ,  A61K39/395 ,  C07K1/14

CPC classification number: C07K16/2887 ,  A61K39/39591 ,  C07K1/14 ,  C07K2317/24

Abstract: The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

公开(公告)号：US20210095040A1

公开(公告)日：2021-04-01

申请号：US17124314

申请日：2020-12-16

Applicant: Genentech, Inc.

Inventor： Yung-Hsiang KAO ,  Michael W. LAIRD ,  Melody Trexler SCHMIDT ,  Rita L. WONG ,  Daniel P. HEWITT

IPC: C07K16/28 ,  A61K39/395 ,  C07K1/14

Abstract: The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

公开(公告)号：US20250137027A1

公开(公告)日：2025-05-01

申请号：US18990600

申请日：2024-12-20

Applicant: Genentech, Inc.

Inventor： Michael W. LAIRD ,  Richard ST. JOHN ,  Jane V. GUNSON ,  Kimberly KALEAS ,  Deepa NADARAJAH ,  Rachel L.E. ADAMS ,  Bradley R. SNEDECOR

IPC: C12P21/00 ,  C07K16/00

Abstract: The present invention contemplates a method of producing a recombinant protein comprising (a) fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, and (b) harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm. The present invention also contemplates a method of producing a recombinant protein comprising (a) fermenting a menE gene-deleted prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, (b) harvesting said recombinant protein; and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm, and wherein the recombinant protein yield is increased by about 20% or greater.

公开(公告)号：US20210188993A1

公开(公告)日：2021-06-24

申请号：US17087313

申请日：2020-11-02

Applicant: Genentech, Inc.

Inventor： Yung-Hsiang KAO ,  Michael W. LAIRD ,  Melody Trexler SCHMIDT ,  Rita L. WONG ,  Daniel P. HEWITT

IPC: C07K16/28 ,  A61K39/395 ,  C07K1/14

Abstract: The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

公开(公告)号：US20180291411A1

公开(公告)日：2018-10-11

申请号：US15908526

申请日：2018-02-28

Applicant: Genentech, Inc.

Inventor： Michael W. LAIRD ,  Richard ST. JOHN ,  Jane V. GUNSON ,  Kimberly KALEAS ,  Deepa NADARAJAH ,  Rachel L.E. ADAMS ,  Bradley R. SNEDECOR

IPC: C12P21/00 ,  C07K16/00

Abstract: The present invention contemplates a method of producing a recombinant protein comprising (a) fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, and (b) harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm. The present invention also contemplates a method of producing a recombinant protein comprising (a) fermenting a menE gene-deleted prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, (b) harvesting said recombinant protein; and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm, and wherein the recombinant protein yield is increased by about 20% or greater.

公开(公告)号：US20140128575A1

公开(公告)日：2014-05-08

申请号：US14043758

申请日：2013-10-01

Applicant: Genentech, Inc.

Inventor： Yung-Hsiang KAO ,  Michael W. LAIRD ,  Melody Trexler SCHMIDT ,  Rita L. WONG ,  Daniel P. HEWITT

IPC: C07K16/28

CPC classification number: C07K16/2887 ,  A61K39/39591 ,  C07K1/14 ,  C07K2317/24

Abstract: The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

Abstract translation: 本发明涉及在重组生产含二硫化物的多肽期间防止二硫键还原的方法和手段。 特别地，本发明涉及在从重组宿主细胞培养物中收获含二硫键的多肽（包括抗体）期间预防二硫键还原。

公开(公告)号：US20240301080A1

公开(公告)日：2024-09-12

申请号：US18648209

申请日：2024-04-26

Applicant: Genentech, Inc.

Inventor： Yung-Hsiang KAO ,  Michael W. LAIRD ,  Melody Trexler SCHMIDT ,  Rita L. WONG ,  Daniel P. HEWITT

IPC: C07K16/28 ,  A61K39/395 ,  C07K1/14

CPC classification number: C07K16/2887 ,  A61K39/39591 ,  C07K1/14 ,  C07K2317/24

Abstract: The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

公开(公告)号：US20240150486A1

公开(公告)日：2024-05-09

申请号：US18410876

申请日：2024-01-11

Applicant: Genentech, Inc.

Inventor： Yung-Hsiang KAO ,  Michael W. LAIRD ,  Melody Trexler SCHMIDT ,  Rita L. WONG ,  Daniel P. HEWITT

IPC: C07K16/28 ,  A61K39/395 ,  C07K1/14

CPC classification number: C07K16/2887 ,  A61K39/39591 ,  C07K1/14 ,  C07K2317/24

Abstract: The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

公开(公告)号：US20200347145A1

公开(公告)日：2020-11-05

申请号：US16847317

申请日：2020-04-13

Applicant: Genentech, Inc.

Inventor： Yung-Hsiang KAO ,  Michael W. LAIRD ,  Melody Trexler SCHMIDT ,  Rita L. WONG ,  Daniel P. HEWITT

IPC: C07K16/28 ,  A61K39/395 ,  C07K1/14

Abstract: The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.

公开(公告)号：US20180230506A1

公开(公告)日：2018-08-16

申请号：US15951895

申请日：2018-04-12

Applicant: Genentech, Inc.

Inventor： Michael W. LAIRD ,  Karthik VEERAVALLI

IPC: C12P21/00 ,  C12P21/02 ,  C12N15/70 ,  C12N9/10 ,  C07K16/18 ,  C07K16/40 ,  C07K16/32 ,  C07K16/28 ,  C07K16/22

Abstract: The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein.