公开(公告)号：US09388384B2

公开(公告)日：2016-07-12

申请号：US13504881

申请日：2009-10-28

申请人： Hyo Soo Kim ,  Hyun Jae Kang ,  Eun Ju Lee ,  Young Bae Park

发明人： Hyo Soo Kim ,  Hyun Jae Kang ,  Eun Ju Lee ,  Young Bae Park

IPC分类号： C12N5/00 ,  C12N5/0775 ,  A61K35/48

CPC分类号： C12N5/0662 ,  A61K35/48 ,  C12N2500/38 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/39 ,  C12N2506/02

摘要： Provided is a method for producing mesenchymal stem cells from human pluripotent stem cells, the method including: a) forming embryonic bodies from human pluripotent stem cells; b) attaching the embryonic bodies to a culture dish to induce natural differentiation of the embryonic bodies into mesenchymal stem cells; and c) performing continuous proliferative culturing of the mesenchymal stem cells while still maintaining the identity of the mesenchymal stem cells. Also, provided is a standardized method for inducing differentiation of mesenchymal stem cells, which can be broadly applied to all human pluripotent stem cells regardless of a difference in the genetic background thereof. Ultimately, the present invention can continuously mass-produces the mesenchymal stem cells necessary for regenerative medicine and cell therapy by using human pluripotent stem cells, thereby realizing practical uses of cell therapy products, and further the present invention is expected to highly contribute to treatments of incurable diseases, such as cardiovascular diseases and neurological disorders.

摘要翻译： 提供一种从人多能干细胞产生间充质干细胞的方法，所述方法包括：a）从人多能干细胞形成胚体; b）将胚胎体附着到培养皿中以诱导胚胎体自然分化成间充质干细胞; 和c）对间充质干细胞进行连续增殖培养，同时仍保持间充质干细胞的特性。 此外，提供了诱导间充质干细胞分化的标准化方法，其可以广泛地应用于所有人多能干细胞，而与其遗传背景的差异无关。 最终，本发明可以通过使用人多能干细胞连续批量生产再生医学和细胞治疗所需的间充质干细胞，从而实现细胞治疗产品的实际应用，并且进一步预期本发明对 不治之症，如心血管疾病和神经系统疾病。

公开(公告)号：US20130023048A1

公开(公告)日：2013-01-24

申请号：US13510528

申请日：2010-10-08

申请人： Hyo Soo Kim ,  Hyun Jae Kang ,  Eun Ju Lee

发明人： Hyo Soo Kim ,  Hyun Jae Kang ,  Eun Ju Lee

IPC分类号： C12N5/0775

CPC分类号： C12N5/0667 ,  C12N2500/36 ,  C12N2502/1305

摘要： The present invention relates to a method of inducing high activity of human adipose stem cells, highly active stem cells induced by the method, cell therapeutic agents including the highly active stem cells, and a medium for inducing high activity of human adipose stem cells.The method of the present invention enables a long-term culture of human adipose stem cells while maintaining high activity, production yield and differentiation potency of the stem cells through in vitro culture, even in case culture conditions are not appropriate for mature human adipocytes, security of adipocytes is not guaranteed, or adipocytes are diseased.

摘要翻译： 本发明涉及一种诱导人类脂肪干细胞高活性的方法，由该方法诱导的高活性干细胞，包括高活性干细胞的细胞治疗剂和诱导人类脂肪干细胞高活性的培养基。 本发明的方法能够通过体外培养保持人体脂肪干细胞的长期培养，同时通过体外培养保持干细胞的高活性，产量和分化效能，即使培养条件不适合成熟的人类脂肪细胞，安全性 的脂肪细胞不能保证，或脂肪细胞患病。

公开(公告)号：US20120276625A1

公开(公告)日：2012-11-01

申请号：US13504881

申请日：2009-10-28

申请人： Hyo Soo Kim ,  Hyun Jae Kang ,  Eun Ju Lee ,  Young Bae Park

发明人： Hyo Soo Kim ,  Hyun Jae Kang ,  Eun Ju Lee ,  Young Bae Park

IPC分类号： C12N5/0775

CPC分类号： C12N5/0662 ,  A61K35/48 ,  C12N2500/38 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/39 ,  C12N2506/02

摘要： Provided is a method for producing mesenchymal stem cells from human pluripotent stem cells, the method including: a) forming embryonic bodies from human pluripotent stem cells; b) attaching the embryonic bodies to a culture dish to induce natural differentiation of the embryonic bodies into mesenchymal stem cells; and c) performing continuous proliferative culturing of the mesenchymal stem cells while still maintaining the identity of the mesenchymal stem cells. Also, provided is a standardized method for inducing differentiation of mesenchymal stem cells, which can be broadly applied to all human pluripotent stem cells regardless of a difference in the genetic background thereof. Ultimately, the present invention can continuously mass-produces the mesenchymal stem cells necessary for regenerative medicine and cell therapy by using human pluripotent stem cells, thereby realizing practical uses of cell therapy products, and further the present invention is expected to highly contribute to treatments of incurable diseases, such as cardiovascular diseases and neurological disorders.

摘要翻译： 提供一种从人多能干细胞产生间充质干细胞的方法，所述方法包括：a）从人多能干细胞形成胚体; b）将胚胎体附着到培养皿中以诱导胚胎体自然分化成间充质干细胞; 和c）对间充质干细胞进行连续增殖培养，同时仍保持间充质干细胞的特性。 此外，提供了诱导间充质干细胞分化的标准化方法，其可以广泛地应用于所有人多能干细胞，而与其遗传背景的差异无关。 最终，本发明可以通过使用人多能干细胞连续批量生产再生医学和细胞治疗所需的间充质干细胞，从而实现细胞治疗产品的实际应用，并且进一步预期本发明对 不治之症，如心血管疾病和神经系统疾病。

公开(公告)号：US08697441B2

公开(公告)日：2014-04-15

申请号：US13510528

申请日：2010-10-08

申请人： Hyo Soo Kim ,  Hyun Jae Kang ,  Eun Ju Lee

发明人： Hyo Soo Kim ,  Hyun Jae Kang ,  Eun Ju Lee

IPC分类号： C12N5/071

CPC分类号： C12N5/0667 ,  C12N2500/36 ,  C12N2502/1305

摘要： The present invention relates to a method of inducing high activity of human adipose stem cells, highly active stem cells induced by the method, cell therapeutic agents including the highly active stem cells, and a medium for inducing high activity of human adipose stem cells. The method of the present invention enables a long-term culture of human adipose stem cells while maintaining high activity, production yield and differentiation potency of the stem cells through in vitro culture, even in case culture conditions are not appropriate for mature human adipocytes, security of adipocytes is not guaranteed, or adipocytes are diseased.

摘要翻译： 本发明涉及一种诱导人类脂肪干细胞高活性的方法，由该方法诱导的高活性干细胞，包括高活性干细胞的细胞治疗剂和诱导人类脂肪干细胞高活性的培养基。 本发明的方法能够通过体外培养保持人体脂肪干细胞的长期培养，同时通过体外培养保持干细胞的高活性，产量和分化效能，即使培养条件不适合成熟的人类脂肪细胞，安全性 的脂肪细胞不能保证，或脂肪细胞患病。

公开(公告)号：US08369931B2

公开(公告)日：2013-02-05

申请号：US12666181

申请日：2008-06-23

申请人： Sun Ha Paek ,  Yong Hoon Lim ,  Hyun Jae Kang ,  Se Ho Shin ,  Eun Ju Choi ,  Cheol Young Kim

发明人： Sun Ha Paek ,  Yong Hoon Lim ,  Hyun Jae Kang ,  Se Ho Shin ,  Eun Ju Choi ,  Cheol Young Kim

IPC分类号： A61B5/00

CPC分类号： A61B90/36 ,  A61B2090/364 ,  A61N1/0534

摘要： The present invention relates to a method of determining the position of a deep brain stimulation (DBS) electrode which finds the position of the DBS electrode with respect to a deep brain target region, by using a first volume data set containing information on the deep brain target region and a second volume data set containing information on the DBS electrode implanted toward the deep brain target region, and which includes: a first step of generating a subvolume of the deep brain target region from the first volume data set, and also generating a subvolume of the DBS electrode from the second volume data set; and a second step of overlapping and displaying the subvolume of the deep brain target region and the subvolume of the DBS electrode.

摘要翻译： 本发明涉及一种通过使用包含深脑信息的第一体积数据集来确定深部脑刺激（DBS）电极的位置的方法，其找到DBS电极相对于深脑靶区域的位置 目标区域和第二体积数据集，其包含植入到深脑靶区域的DBS电极上的信息，其包括：第一步骤，从第一体积数据集生成深脑对象区域的子体积，并且还生成 DBS电极的子体积从第二体积数据集合; 以及重叠和显示DBS电极的深脑靶区域和子体积的子体积的第二步骤。

公开(公告)号：US06716746B1

公开(公告)日：2004-04-06

申请号：US09640754

申请日：2000-08-18

申请人： In Sung Kim ,  Joon Soo Park ,  Jung Hyeon Lee ,  Hyun Jae Kang

发明人： In Sung Kim ,  Joon Soo Park ,  Jung Hyeon Lee ,  Hyun Jae Kang

IPC分类号： H01L214763

CPC分类号： H01L21/76897

摘要： A semiconductor device includes a conductive region and line, and a contact plug electrically connecting the line and the region. The line is connected to the region via sidewalls of the plug, and the region is connected to the line via the bottom of the plug. The cross-sectional area of the plug decreases in a direction from an upper to lower portion thereof. In a first method of fabricating a semiconductor device having a self-aligned contact, the plug is formed after the line is formed in an interlayer dielectric layer. Portions of the dielectric layer and line are etched to form a contact hole in which the plug is formed. In a second method, a line having a gap therein is formed in an interlayer dielectric layer. Portions of the dielectric layer, including the gap in the line, are etched to form the contact hole.

摘要翻译： 半导体器件包括导电区域和线路，以及电连接线路和区域的接触插头。 线路通过插头的侧壁连接到该区域，并且该区域经由插头的底部连接到线路。 塞子的横截面积从其上部向下部的方向减小。 在制造具有自对准接触的半导体器件的第一种方法中，在将线形成在层间电介质层中之后形成插塞。 蚀刻电介质层和线的一部分以形成其中形成插头的接触孔。 在第二种方法中，在层间绝缘层中形成具有间隙的线。 包括线中的间隙的电介质层的一部分被蚀刻以形成接触孔。

公开(公告)号：US09900053B2

公开(公告)日：2018-02-20

申请号：US14881797

申请日：2015-10-13

申请人： Jun Ho Kim ,  Hyun Jae Kang ,  Min Woo Lee ,  Hyoung Hwan Roh

发明人： Jun Ho Kim ,  Hyun Jae Kang ,  Min Woo Lee ,  Hyoung Hwan Roh

IPC分类号： H04B5/00

CPC分类号： H04B5/0031 ,  H04B5/0056

摘要： A near-field communication (NFC) card reader may include a monitor configured to measure an amplitude of a magnetic field induced by an antenna; a gain controller configured to determine an amplification gain based on the measured amplitude of the magnetic field and output a gain control signal; a signal restoration unit configured to receive a carrier signal and a data signal that overlaps with the carrier signal via the antenna, and restore the data signal from the received signals; and a variable-gain amplifier configured to amplify the data signal restored by the signal restoration unit according to the gain control signal.

公开(公告)号：US20100324410A1

公开(公告)日：2010-12-23

申请号：US12666181

申请日：2008-06-23

申请人： Sun Ha Paek ,  Yong Hoon Lim ,  Hyun Jae Kang ,  Se Ho Shin ,  Eun Ju Choi ,  Cheol Young Kim

发明人： Sun Ha Paek ,  Yong Hoon Lim ,  Hyun Jae Kang ,  Se Ho Shin ,  Eun Ju Choi ,  Cheol Young Kim

IPC分类号： A61B5/055 ,  G06T7/00 ,  A61B5/05

CPC分类号： A61B90/36 ,  A61B2090/364 ,  A61N1/0534

摘要： The present invention relates to a method of determining the position of a deep brain stimulation (DBS) electrode which finds the position of the DBS electrode with respect to a deep brain target region, by using a first volume data set containing information on the deep brain target region and a second volume data set containing information on the DBS electrode implanted toward the deep brain target region, and which includes: a first step of generating a subvolume of the deep brain target region from the first volume data set, and also generating a subvolume of the DBS electrode from the second volume data set; and a second step of overlapping and displaying the subvolume of the deep brain target region and the subvolume of the DBS electrode.

摘要翻译： 本发明涉及一种通过使用包含深脑信息的第一体积数据集来确定深部脑刺激（DBS）电极的位置的方法，其找到DBS电极相对于深脑靶区域的位置 目标区域和第二体积数据集，其包含植入到深脑靶区域的DBS电极上的信息，其包括：第一步骤，从第一体积数据集生成深脑对象区域的子体积，并且还生成 DBS电极的子体积从第二体积数据集合; 以及重叠和显示DBS电极的深脑靶区域和子体积的子体积的第二步骤。