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公开(公告)号：US20060078936A1
公开(公告)日：2006-04-13
申请号：US11284307
申请日：2005-11-19
申请人： Jennifer Grenier , David Marshall , James Prudent , Craig Richmond , Eric Roesch , Christopher Scherrer , Christopher Sherrill , Jerod Ptacin
发明人： Jennifer Grenier , David Marshall , James Prudent , Craig Richmond , Eric Roesch , Christopher Scherrer , Christopher Sherrill , Jerod Ptacin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6832 , C12Q1/6823 , C12Q1/6827 , C12Q1/6834 , C12Q1/6858 , C12Q2600/156 , C12Q2565/1015 , C12Q2525/101 , C12Q2525/161 , C12Q2525/117 , C12Q2565/537
摘要： Solid support assays using non-standard bases are described. A capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target oligonucleotide. In some instances, the molecular recognition sequence includes one or more non-standard bases and hybridizes to a complementary tagging sequence of the target oligonucleotide. In other instances, incorporation of a non-standard base (e.g., via PCR or ligation) is used in the assay.
摘要翻译： 描述了使用非标准碱的固体支持试验。 包含分子识别序列的捕获寡核苷酸与固相支持物连接并与靶寡核苷酸杂交。 在一些情况下，分子识别序列包括一个或多个非标准碱基并与靶寡核苷酸的互补标记序列杂交。 在其他情况下，在测定中使用非标准碱基的掺入（例如经由PCR或连接）。
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公开(公告)号：US07892796B2
公开(公告)日：2011-02-22
申请号：US11284307
申请日：2005-11-19
申请人： James Prudent , David Marshall , Christopher Sherrill , Eric Roesch , Craig Richmond , Christopher Scherrer , Jennifer Grenier , Jerod Ptacin
发明人： James Prudent , David Marshall , Christopher Sherrill , Eric Roesch , Craig Richmond , Christopher Scherrer , Jennifer Grenier , Jerod Ptacin
CPC分类号： C12Q1/6832 , C12Q1/6823 , C12Q1/6827 , C12Q1/6834 , C12Q1/6858 , C12Q2600/156 , C12Q2565/1015 , C12Q2525/101 , C12Q2525/161 , C12Q2525/117 , C12Q2565/537
摘要： Solid support assays using non-standard bases are described. A capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target oligonucleotide. In some instances, the molecular recognition sequence includes one or more non-standard bases and hybridizes to a complementary tagging sequence of the target oligonucleotide. In other instances, incorporation of a non-standard base (e.g., via PCR or ligation) is used in the assay.
摘要翻译： 描述了使用非标准碱的固体支持试验。 包含分子识别序列的捕获寡核苷酸与固相支持物连接并与靶寡核苷酸杂交。 在一些情况下，分子识别序列包括一个或多个非标准碱基并与靶寡核苷酸的互补标记序列杂交。 在其他情况下，在测定中使用非标准碱基的掺入（例如经由PCR或连接）。
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公开(公告)号：US20070087361A1
公开(公告)日：2007-04-19
申请号：US11490319
申请日：2006-07-20
申请人： Jennifer Grenier , David Marshall , James Prudent , Craig Richmond , Eric Roesch , Christopher Scherrer , Christopher Sherrill , Jerod Ptacin
发明人： Jennifer Grenier , David Marshall , James Prudent , Craig Richmond , Eric Roesch , Christopher Scherrer , Christopher Sherrill , Jerod Ptacin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6832 , C12Q1/6823 , C12Q1/6827 , C12Q1/6834 , C12Q1/6858 , C12Q2600/156 , C12Q2565/1015 , C12Q2525/101 , C12Q2525/161 , C12Q2525/117 , C12Q2565/537
摘要： Solid support assays using non-standard bases are described. A capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target. In some instances, the molecular recognition sequence includes one or more non-standard bases and hybridizes to a complementary tagging sequence of the target oligonucleotide. In other instances, incorporation of a non-standard base (e.g., via PCR or ligation) is used in the assay.
摘要翻译： 描述了使用非标准碱的固体支持试验。 包含分子识别序列的捕获寡核苷酸附着在固体支持物上并与靶杂交。 在一些情况下，分子识别序列包括一个或多个非标准碱基并与靶寡核苷酸的互补标记序列杂交。 在其他情况下，在测定中使用非标准碱基的掺入（例如经由PCR或连接）。
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公开(公告)号：US20070031885A1
公开(公告)日：2007-02-08
申请号：US11546631
申请日：2006-10-12
申请人： David Marshall , James Prudent , Christopher Sherrill , Gideon Shapiro , Jennifer Grenier , Craig Richmond , Simona Jurczyk , Jerod Ptacin
发明人： David Marshall , James Prudent , Christopher Sherrill , Gideon Shapiro , Jennifer Grenier , Craig Richmond , Simona Jurczyk , Jerod Ptacin
CPC分类号： C12Q1/6853 , C12Q1/6823 , C12Q1/6827 , C12Q1/6832 , C12Q1/6834 , C12Q1/6858 , C12Q2600/156 , C12Q2565/1015 , C12Q2525/101 , C12Q2525/161
摘要： Assays using non-natural bases are described. In one embodiment, the method involves contacting a sample suspected of containing the target nucleic acid with a polymerase and first and second primers; amplifying the target nucleic acid, if present in the sample, by PCR using the first and second primers to generate an amplification product having a double-stranded region and a single-stranded region that comprises the non-natural base; contacting the sample with a reporter comprising a label and a non-natural base that is complementary to the non-natural base of the single-stranded region; annealing at least a portion of the reporter to the single-stranded region of the amplification product; and correlating a signal of the label with the presence of the target nucleic acid in the sample. The invention also provides corresponding kits for use in detecting target nucleic acids in a sample.
摘要翻译： 描述了使用非天然碱的测定法。 在一个实施方案中，所述方法包括使怀疑含有靶核酸的样品与聚合酶和第一和第二引物接触; 如果存在于样品中，则使用第一和第二引物通过PCR扩增靶核酸以产生具有双链区域和包含非天然碱基的单链区域的扩增产物; 使样品与包含与单链区域的非天然碱基互补的标记物和非天然碱基的报告子接触; 将至少一部分报告物退火至扩增产物的单链区域; 并将标记的信号与样品中靶核酸的存在相关联。 本发明还提供用于检测样品中靶核酸的相应试剂盒。
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公开(公告)号：US20060252091A1
公开(公告)日：2006-11-09
申请号：US11486841
申请日：2006-07-14
申请人： David Marshall , James Prudent , Christopher Sherrill , Gideon Shapiro , Jennifer Grenier , Craig Richmond , Simona Jurczyk , Jerod Ptacin
发明人： David Marshall , James Prudent , Christopher Sherrill , Gideon Shapiro , Jennifer Grenier , Craig Richmond , Simona Jurczyk , Jerod Ptacin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6853 , C12Q1/6823 , C12Q1/6827 , C12Q1/6832 , C12Q1/6834 , C12Q1/6858 , C12Q2600/156 , C12Q2565/1015 , C12Q2525/101 , C12Q2525/161
摘要： Assays using non-natural bases are described. In one embodiment, the method involves contacting a sample suspected of containing the target nucleic acid with a polymerase and first and second primers; amplifying the target nucleic acid, if present in the sample, by PCR using the first and second primers to generate an amplification product having a double-stranded region and a single-stranded region that comprises the non-natural base; contacting the sample with a reporter comprising a label and a non-natural base that is complementary to the non-natural base of the single-stranded region; annealing at least a portion of the reporter to the single-stranded region of the amplification product; and correlating a signal of the label with the presence of the target nucleic acid in the sample. The invention also provides corresponding kits for use in detecting target nucleic acids in a sample.
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