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1.发明授权Mannanase enzymes, genes coding for them and a method for isolating the genes, as well as a process for bleaching of lignocellulosic pulp 失效甘露聚糖酶，编码它们的基因和分离基因的方法，以及木质纤维素浆的漂白方法
公开(公告)号：US5661021A
公开(公告)日：1997-08-26
申请号：US341568
申请日：1994-11-22
申请人： Johanna Buchert , Matti Siika-aho , Liisa Viikari , Merja Penttila , Anu Saloheimo , Marjatta Ranua
发明人： Johanna Buchert , Matti Siika-aho , Liisa Viikari , Merja Penttila , Anu Saloheimo , Marjatta Ranua
CPC分类号： C12Y302/01101 , C12N9/2488 , C12Y302/01025 , C12Y302/01078 , D21C5/005
摘要： The present invention relates to a DNA sequence, which codes for endomannanase produced by fungi of the genus Trichoderma and transferred into a yeast or fungus strain induces that strain to produce endomannanase, as well as to a method for isolating genes coding for endomannanases. The invention also relates to vectors, yeast strains and fungal strains containing the DNA sequence. Furthermore, the invention provides an enzyme product containing at least one endomannanase, which contains at least one of the following endomannanases produced by fungi of the Trichoderma genus: an enzyme having mannanase activity and an isoelectric point (pI) of about 3.8, an enzyme having mannanase activity and a pI of about 4.1, an enzyme having mannanase activity and a pI of about 4.5, an enzyme having mannanase activity and a pI of about 5.4 and an enzyme having mannanase activity and a pI of about 6.5, the isoelectric points being determined by isoelectric focusing. The endomannanase enzyme and enzyme products according to the invention can be used for hydrolyzation of mannopolymers, in particular in connection with bleaching of lignocellulosic pulps.
摘要翻译： PCT No.PCT / FI93 / 00219 Sec。 371日期：1994年11月22日 102（e）1994年11月22日日期PCT提交1993年5月24日PCT公布。 出版物WO93 / 24622 日期：1993年12月9日本发明涉及一种DNA序列，其编码由木霉属真菌产生并转移到酵母或真菌菌株中的内切葡聚糖酶诱导该菌株产生内切葡聚糖酶，以及分离基因编码方法 内分泌酶。 本发明还涉及含有DNA序列的载体，酵母菌株和真菌菌株。 此外，本发明提供一种含有至少一种含有甘露糖醛酸酶的酶产物，其含有由木霉属真菌产生的至少一种以下的甘露聚糖酶：具有约3.8的甘露聚糖酶活性和等电点（pI）的酶，其具有 甘露聚糖酶活性和约4.1的pI，具有约4.5的甘露聚糖酶活性和pI的酶，具有甘露聚糖酶活性和pI约为5.4的酶和具有约6.5的甘露聚糖酶活性和pI的酶，测定等电点 通过等电聚焦。 根据本发明的内切单体酶和酶产物可用于水解甘露聚合物，特别是与木质纤维素纸浆的漂白有关。
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2.发明授权Mannanase enzymes, genes coding for them and a method for isolating the genes, as well as a process for bleaching of lignocellulosic pulp 失效甘露聚糖酶，编码它们的基因和分离基因的方法，以及木质纤维素浆的漂白方法
公开(公告)号：US5854047A
公开(公告)日：1998-12-29
申请号：US911020
申请日：1997-08-13
申请人： Johanna Buchert , Matti Siika-aho , Liisa Viikari , Merja Penttila , Anu Saloheimo , Marjatta Ranua
发明人： Johanna Buchert , Matti Siika-aho , Liisa Viikari , Merja Penttila , Anu Saloheimo , Marjatta Ranua
CPC分类号： C12Y302/01101 , C12N9/2488 , C12Y302/01025 , C12Y302/01078 , D21C5/005
摘要： The present invention relates to a DNA sequence, which codes for endomannanase produced by fungi of the genus Trichoderma and transferred into a yeast or fungus strain induces that strain to produce endomannanase, as well as to a method for isolating genes coding for endomannanases. The invention also relates to vectors, yeast strains and fungal strains containing the DNA sequence. Furthermore, the invention provides an enzyme product containing at least one endomannanase, which contains at least one of the following endomannanases produced by fungi of the Trichoderma genus: an enzyme having mannanase activity and an isoelectric point (pI) of about 3.8, an enzyme having mannanase activity and a pI of about 4.1, an enzyme having mannanase activity and a pI of about 4.5, an enzyme having mannanase activity and a pI of about 5.4 and an enzyme having mannanase activity and a pI of about 6.5, the isoelectric points being determined by isoelectric focusing. The endomannanase enzyme and enzyme products according to the invention can be used for hydrolyzation of mannopolymers, in particular in connection with bleaching of lignocellulosic pulps.
摘要翻译： 本发明涉及一种DNA序列，其编码由木霉属真菌产生并转移到酵母或真菌菌株中的内切葡聚糖酶诱导该菌株产生胞外单胞菌素酶，以及分离编码内切甘露聚糖酶的基因的方法。 本发明还涉及含有DNA序列的载体，酵母菌株和真菌菌株。 此外，本发明提供一种含有至少一种含有甘露糖醛酸酶的酶产物，其含有由木霉属真菌产生的至少一种以下的甘露聚糖酶：具有约3.8的甘露聚糖酶活性和等电点（pI）的酶，其具有 甘露聚糖酶活性和约4.1的pI，具有约4.5的甘露聚糖酶活性和pI的酶，具有甘露聚糖酶活性和pI约为5.4的酶和具有约6.5的甘露聚糖酶活性和pI的酶，测定等电点 通过等电聚焦。 根据本发明的内切单体酶和酶产物可用于水解甘露聚合物，特别是与木质纤维素纸浆的漂白有关。
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3.发明授权Method of modifying a xylan-containing carbohydrate substrate having hexenuronic acid groups attached to the xylan 失效修饰具有连接到木聚糖上的己烯醛酸基团的含木聚糖的碳水化合物底物的方法公开(公告)号：US06830655B2
公开(公告)日：2004-12-14
申请号：US08750069
申请日：1997-02-06
申请人： Matti Siika-Aho , Johanna Buchert , Tapani Vuorinen , Anita Teleman , Maija Tenkanen , Michael Bailey , Liisa Viikari
发明人： Matti Siika-Aho , Johanna Buchert , Tapani Vuorinen , Anita Teleman , Maija Tenkanen , Michael Bailey , Liisa Viikari
IPC分类号： D21H2502
CPC分类号： C12Y302/01031 , C12N9/2402 , C12P19/14 , D21C5/005 , Y10S435/814
摘要： The present invention concerns a method for enzymatic treatment of lignocellulosic materials which contain xylan-polymers, such as cellulose kraft pulps. According to a method of the present kind, at least a part of the hexenuronic acid groups present in the material is selectively removed in order to remove metal ions from the pulp, to change the surface charge thereof, to improve the brightness stability of the pulp and to render the material more suitable for enzymatic treatment.
摘要翻译： 本发明涉及用于酶处理木质纤维素材料的方法，所述木素纤维素材料含有木聚糖聚合物，例如纤维素硫酸盐纸浆。 根据本发明的方法，选择性地除去材料中存在的己烯醛酸基团的至少一部分，以从纸浆中除去金属离子，以改变其表面电荷，以改善纸浆的亮度稳定性 并使材料更适合于酶处理。
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4.发明申请Method for reducing brightness reversion of mechanical pulps and high-yield chemical pulps 审中-公开降低机械纸浆和高产量化学纸浆的亮度反转的方法公开(公告)号：US20070163735A1
公开(公告)日：2007-07-19
申请号：US10583340
申请日：2004-12-23
申请人： Johanna Buchert , Liisa Viikari , Stina Gronqvist , Mikael Svedman , Arto Paren , Veli-Matti Vuorenpalo
发明人： Johanna Buchert , Liisa Viikari , Stina Gronqvist , Mikael Svedman , Arto Paren , Veli-Matti Vuorenpalo
IPC分类号： D21C9/00
CPC分类号： D21C9/005 , D21C5/005 , D21H11/20 , D21H21/143
摘要： The present invention concerns a process for reducing the susceptibility of lignocellulosic material to unwanted yellowing, particularly yellowing caused by light and heat. According to the invention, the fibres are activated enzymatically or chemically and then contacted with a modifying agent capable of bonding to the oxidized fibre material, rendering the lignocellulosic fibre material improved resistance to brightness reversion. By means of the invention, brightness reversion caused by light or heat or a combination thereof can be retarded and even stopped.
摘要翻译： 本发明涉及一种降低木质纤维素材料对不希望变黄的敏感性的方法，特别是由光和热引起的变黄。 根据本发明，纤维被酶促或化学活化，然后与能够结合到氧化纤维材料的改性剂接触，使木质纤维素纤维材料改善了对亮度回复的抵抗力。 通过本发明，可以延迟甚至停止由光或热或其组合导致的亮度反转。
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公开(公告)号：US20070164468A1
公开(公告)日：2007-07-19
申请号：US10583339
申请日：2004-12-23
申请人： Johanna Buchert , Stina Gronqvist , Hannu Mikkonen , Tarja Oksanen , Soili Peltonen , Anna Suurnakki , Liisa Viikari
发明人： Johanna Buchert , Stina Gronqvist , Hannu Mikkonen , Tarja Oksanen , Soili Peltonen , Anna Suurnakki , Liisa Viikari
IPC分类号： B32B37/00
CPC分类号： D21H11/20 , C08F251/02 , C08L51/02 , D21H15/10 , C08L2666/02
摘要： The present invention concerns a process for producing fibre composites. In particular, the invention provides a novel way of producing biodegradable composites comprising a hydrophobic polymer material and a reinforcing component of fibres derived from plant materials. Composite material produced by means of the present invention has improved strength properties and enhanced adhesion between the bifunctional fibre and the natural or synthetic polymer.
摘要翻译： 本发明涉及一种生产纤维复合材料的方法。 特别地，本发明提供了生产可生物降解复合材料的新方法，其包括疏水性聚合物材料和源自植物材料的纤维的增强组分。 通过本发明生产的复合材料具有改善的强度性能和双官能纤维与天然或合成聚合物之间增强的粘附性。
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公开(公告)号：US20070143932A1
公开(公告)日：2007-06-28
申请号：US10583849
申请日：2004-12-23
IPC分类号： C08F251/02 , D21H11/20 , H01B1/12
摘要： The present invention relates to fibre compositions. In particular, the present invention concerns a process for producing a fibre composition comprising a lignocellulosic fibre material and a synthetic, electrically conductive polymer formed by polymerized monomers. The invention provides for good adhesion between the fibre matrix and the polymer, because the monomer is polymerized directly on the fibre. The conductivity of the polymer is improved and the electrical properties and conductivity levels of the modified fibre can be adjusted by changing the amounts of the electrically conductive polymer.
摘要翻译： 本发明涉及纤维组合物。 特别地，本发明涉及一种生产包含木质纤维素纤维材料和由聚合单体形成的合成导电聚合物的纤维组合物的方法。 本发明提供了纤维基质和聚合物之间良好的粘附性，因为单体直接在纤维上聚合。 提高聚合物的导电性，并且可以通过改变导电聚合物的量来调节改性纤维的电性能和电导率水平。
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公开(公告)号：US20070131362A1
公开(公告)日：2007-06-14
申请号：US10583712
申请日：2004-12-23
申请人： Johanna Buchert , Hannu Mikkonen , Soili Peltonen , Liisa Viikari , Stina Gronqvist , Tarja Oksanen , Anna Suurnakki
发明人： Johanna Buchert , Hannu Mikkonen , Soili Peltonen , Liisa Viikari , Stina Gronqvist , Tarja Oksanen , Anna Suurnakki
IPC分类号： D21H11/20 , D21H21/14 , C08F251/02
CPC分类号： D21C9/002 , C08F251/02 , C08L51/02 , D21H11/20 , D21H21/40 , C08L2666/02
摘要： The present invention concerns a process for modifying a lignocellulosic fibre material. In particular, the present invention gives new properties to the fibrous matrix of the material. This is achieved by producing fibrous products with modified properties by activating the fibres of the matrix with an oxidizing agent and attaching compounds to the activated fibre in order to incorporate desired, pre-selected properties in to the fibre matrix. The invention makes possible to produce novel kinds of fibrous materials having practically a large variety of properties. Examples of such properties include hydrophobic hydrophilic character.
摘要翻译： 本发明涉及一种改性木质纤维素纤维材料的方法。 特别地，本发明给出材料的纤维基质的新特性。 这通过用氧化剂活化基质的纤维并将化合物连接到活化的纤维上以产生纤维基质中所需的预先选择的性质来制备具有改性特性的纤维制品来实现。 本发明使得可以生产具有实际上具有多种性质的新型纤维材料。 这些性质的实例包括疏水亲水性。
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公开(公告)号：US08546115B2
公开(公告)日：2013-10-01
申请号：US12668313
申请日：2008-07-08
申请人： Johanna Buchert , Tiina Nakari-Setälä , Pasi Halonen , Hanna Kontkanen , Ann Westerholm-Parvinen , Marjaana Rättö
发明人： Johanna Buchert , Tiina Nakari-Setälä , Pasi Halonen , Hanna Kontkanen , Ann Westerholm-Parvinen , Marjaana Rättö
IPC分类号： C12N9/18
CPC分类号： C12N9/18 , A23L5/25 , C11D3/386 , C12Y301/01 , C12Y301/01074 , D06M16/003 , D06M2101/32
摘要： The present invention relates to polyesterases having cutinase and/or suberinase activity obtainable from Coprinus and Trichoderma. The invention further relates to a method for producing the polyesterases, and to polynucleotides, vectors and host cells used therein. The enzymes are useful in hydrolysing cutin, suberin and other polyesters for example in treating agricultural or food raw materials, or wood raw materials, pulp and paper products and waste, and in modifying polyester fibers, or in laundry and dish applications.
摘要翻译： 本发明涉及具有从鬼伞属和木霉属获得的角质酶和/或辛草林酶活性的聚酯酶。 本发明还涉及生产聚酯酶的方法，以及其中使用的多核苷酸，载体和宿主细胞。 酶可用于水解角质，桧木和其他聚酯，例如用于处理农业或食品原料，或木材原料，纸浆和纸制品和废物，以及改性聚酯纤维，或在洗衣和盘子应用中。
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公开(公告)号：US07910344B2
公开(公告)日：2011-03-22
申请号：US11815988
申请日：2006-02-09
申请人： Kristiina Kruus , Emilia Selinheimo , Karin Autio , Johanna Buchert , Markku Saloheimo , Raija Lantto
发明人： Kristiina Kruus , Emilia Selinheimo , Karin Autio , Johanna Buchert , Markku Saloheimo , Raija Lantto
IPC分类号： C12N9/02
CPC分类号： C12N9/0059 , A23J3/34 , D06M15/15 , D06M16/003 , D06M2101/12
摘要： Extracellular tyrosinases obtainable from Trichoderma spp. and methods for producing them by recombinant technology. The enzymes are particularly useful in cross-linking food proteins.
摘要翻译： 可从木霉属中获得的细胞外酪氨酸酶 以及通过重组技术生产它们的方法。 这些酶特别可用于交联食物蛋白质。
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公开(公告)号：US20080289120A1
公开(公告)日：2008-11-27
申请号：US11629090
申请日：2005-06-13
IPC分类号： C09B62/00
CPC分类号： D06P3/241 , D06M16/003 , D06M2101/34 , D06M2200/00 , D06P3/242 , D06P3/26
摘要： The present invention relates to a method for modifying polyamide. The method comprises that polyamide is contacted with an enzyme preparation comprising an effective amount of protease enzyme in aqueous environment under conditions suitable for the function of the enzyme. The enzyme is preferably selected from the group of aspartic proteases, cysteine proteases and metallo-proteases.
摘要翻译： 本发明涉及一种改性聚酰胺的方法。 该方法包括使聚酰胺与含有有效量的蛋白酶的酶制剂在含水环境中在适于酶功能的条件下接触。 酶优选选自天冬氨酸蛋白酶，半胱氨酸蛋白酶和金属蛋白酶。
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