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公开(公告)号:US09776187B2
公开(公告)日:2017-10-03
申请号:US12229404
申请日:2008-08-22
申请人: John G. Atwood , Adrian Fawcett , Keith S. Ferrara , Paul M. Hetherington , Richard W. Noreiks , Douglas E. Olsen , John R. Widomski , Charles M. Wittmer
发明人: John G. Atwood , Adrian Fawcett , Keith S. Ferrara , Paul M. Hetherington , Richard W. Noreiks , Douglas E. Olsen , John R. Widomski , Charles M. Wittmer
CPC分类号: B01L7/52 , B01L3/50853 , B01L2300/0829 , B01L2300/1822 , B01L2300/1827 , C12Q1/686 , F25B21/04 , F25B2321/021 , G01R27/205 , G05D23/1919 , H01L35/30 , Y10T137/1516
摘要: An instrument for performing highly accurate PCR employing an assembly, a heated cover, and an internal computer, is provided. The assembly is made up of a sample block, a number of Peltier thermal electric devices, and a heat sink, clamped together. A control algorithm manipulates the current supplied to thermoelectric coolers such that the dynamic thermal performance of a block can be controlled so that pre-defined thermal profiles of sample temperature can be executed. The sample temperature is calculated instead of measured using a design specific model and equations. The control software includes calibration diagnostics which permit variation in the performance of thermoelectric coolers from instrument to instrument to be compensated for such that all instruments perform identically. The block/heat sink assembly can be changed to another of the same or different design. The assembly carries the necessary information required to characterize its own performance in an on-board memory device, allowing the assembly to be interchangeable among instruments while retaining its precision operating characteristics.
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公开(公告)号:US09044753B2
公开(公告)日:2015-06-02
申请号:US11654239
申请日:2007-01-17
申请人: John G. Atwood , Adrian Fawcett , Keith S. Ferrara , Paul M. Hetherington , Richard W. Noreiks , Douglas E. Olsen , John R. Widomski , Charles M. Wittmer
发明人: John G. Atwood , Adrian Fawcett , Keith S. Ferrara , Paul M. Hetherington , Richard W. Noreiks , Douglas E. Olsen , John R. Widomski , Charles M. Wittmer
CPC分类号: B01L7/52 , B01L3/50853 , B01L2300/0829 , B01L2300/1822 , B01L2300/1827 , C12Q1/686 , F25B21/04 , F25B2321/021 , G01R27/205 , G05D23/1919 , H01L35/30 , Y10T137/1516
摘要: An instrument for performing highly accurate PCR employing an assembly, a heated cover and an internal computer. The assembly is made up of a sample block, a number of Peltier thermal electric devices and heat sink, clamped together. The sample block temperature is changed exclusively by the thermoelectric devices controlled by the computer. The sample block is of low thermal mass and is constructed of silver. The Peltier devices are designed to provide fast temperature excursions over a wide range. The heat sink has a perimeter trench to minimize edge losses and is adjacent to a continuously variable fan. A perimeter heater is used to improve the thermal uniformity across the sample block to approximately ±0.2° C. A heated platen pushes down onto the tube caps to apply a minimum acceptable force for seating the tubes into the block, ensuring good thermal contact with the block. The force is applied about the periphery of the tube caps to prevent distortion of the caps during thermal cycling. The platen is heated to provided thermal isolation from ambient conditions and to prevent evaporation from the surface of the sample into the upper portion of the sample tube. A control algorithm manipulates the current supplied to the thermoelectric coolers such that the dynamic thermal performance of the block can be controlled so that pre-defined thermal profiles for the sample temperature can be executed. The sample temperature is calculated instead of measured using a design specific model and equations. The control software includes calibration diagnostics which permit variation in the performance of thermoelectric coolers from instrument to instrument to be compensated for such that all instruments perform identically. The block/heat sink assembly can be changed to another of the same or different design. The assembly carries the necessary information required to characterize its own performance in an on-board memory device, allowing the assembly to be interchangeable among instruments while retaining its precision operating characteristics. The instrument has a graphical user interface. The instrument monitors the thermoelectric devices and warns of changes in resistance that may result in failure.
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公开(公告)号:US07133726B1
公开(公告)日:2006-11-07
申请号:US09075392
申请日:1998-05-08
申请人: John G. Atwood , Adrian Fawcett , Keith S. Ferrara , Paul M. Hetherington , Richard W. Noreiks , Douglas E. Olsen , John R. Widomski , Charles M. Wittmer
发明人: John G. Atwood , Adrian Fawcett , Keith S. Ferrara , Paul M. Hetherington , Richard W. Noreiks , Douglas E. Olsen , John R. Widomski , Charles M. Wittmer
IPC分类号: G05B15/00
CPC分类号: B01L7/52 , B01L3/50853 , B01L2300/0829 , B01L2300/1822 , B01L2300/1827 , C12Q1/686 , F25B21/04 , F25B2321/021 , G01R27/205 , G05D23/1919 , H01L35/30 , Y10T137/1516
摘要: An instrument for PCR comprising an assembly, a heated cover, and a computer. The assembly can include a sample block, Peltier devices, and a heat sink, clamped together. The sample block temperature can be changed by controlling the Peltier devices with the computer. A perimeter heater can be used to in prove thermal uniformity. A heated platen can push down onto tube caps to apply a minimum force for seating the tubes. The force can be applied about the periphery of the tube caps to prevent distortion of the caps. The platen can be heated to provide thermal isolation and prevent evaporation from the surface of the sample. The software can include diagnostics to compensate for variation in thermoelectric coolers such that all instruments can perform identically.
摘要翻译: 一种用于PCR的仪器,包括组件,加热盖和计算机。 组件可以包括夹在一起的样品块,珀耳帖装置和散热器。 可以通过用计算机控制珀耳帖装置来更改样品块温度。 周边加热器可用于证明热均匀性。 加热的压板可以向下推到管帽上以施加用于安置管的最小力。 可以围绕管帽的周边施加力以防止帽的变形。 可以加热压板以提供热隔离并防止从样品表面蒸发。 该软件可以包括诊断以补偿热电冷却器的变化,使得所有仪器可以相同地执行。
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公开(公告)号:US08246243B2
公开(公告)日:2012-08-21
申请号:US12686100
申请日:2010-01-12
申请人: John G. Atwood , Adrian Fawcett , Keith S. Ferrara , Paul M. Hetherington , Richard W. Noreiks , Douglas E. Olsen , John R. Widomski , Charles M. Wittmer
发明人: John G. Atwood , Adrian Fawcett , Keith S. Ferrara , Paul M. Hetherington , Richard W. Noreiks , Douglas E. Olsen , John R. Widomski , Charles M. Wittmer
CPC分类号: B01L7/52 , B01L3/50853 , B01L2300/0829 , B01L2300/1822 , B01L2300/1827 , C12Q1/686 , F25B21/04 , F25B2321/021 , G01R27/205 , G05D23/1919 , H01L35/30 , Y10T137/1516
摘要: An instrument for performing highly accurate PCR employing an assembly, a heated cover, and an internal computer, is provided. The assembly is made up of a sample block, a number of Peltier thermal electric devices, and a heat sink, clamped together. A control algorithm manipulates the current supplied to thermoelectric coolers such that the dynamic thermal performance of a block can be controlled so that pre-defined thermal profiles of sample temperature can be executed. The sample temperature is calculated instead of measured using a design specific model and equations. The control software includes calibration diagnostics which permit variation in the performance of thermoelectric coolers from instrument to instrument to be compensated for such that all instruments perform identically. The block/heat sink assembly can be changed to another of the same or different design. The assembly carries the necessary information required to characterize its own performance in an on-board memory device, allowing the assembly to be interchangeable among instruments while retaining its precision operating characteristics.
摘要翻译: 提供了一种使用组件,加热盖和内部计算机执行高精度PCR的仪器。 组件由样品块,多个珀耳帖热电器件和散热器组成,夹在一起。 控制算法控制提供给热电冷却器的电流,使得可以控制块的动态热性能,从而可以执行样本温度的预定义热分布。 计算样品温度,而不是使用设计特定的模型和方程来测量。 控制软件包括校准诊断,其允许从仪器到仪器的热电冷却器的性能的变化被补偿,使得所有仪器执行相同的操作。 块/散热器组件可以改变为相同或不同设计的另一个。 该组件携带在板载存储器件中表征其自身性能所需的必要信息,允许组件在仪器之间可互换,同时保持其精密操作特性。
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公开(公告)号:US07537377B2
公开(公告)日:2009-05-26
申请号:US11057960
申请日:2005-02-15
申请人: John G. Atwood , Adrian Fawcett , Keith S. Ferrara , Paul M. Hetherington , Richard W. Noreiks , Douglas E. Olsen , John R. Widomski , Charles M. Wittmer
发明人: John G. Atwood , Adrian Fawcett , Keith S. Ferrara , Paul M. Hetherington , Richard W. Noreiks , Douglas E. Olsen , John R. Widomski , Charles M. Wittmer
CPC分类号: B01L7/52 , B01L3/50853 , B01L2300/0829 , B01L2300/1822 , B01L2300/1827 , C12Q1/686 , F25B21/04 , F25B2321/021 , G01R27/205 , G05D23/1919 , H01L35/30 , Y10T137/1516
摘要: A method for performing highly accurate PCR employing an assembly, a heated cover and an internal computer. The assembly is made up of a sample block, a number of Peltier thermal electric devices and heat sink, clamped together. The sample block temperature is changed exclusively by the thermoelectric devices controlled by the computer. The control software includes calibration diagnostics which permit variation in the performance of thermoelectric coolers from instrument to instrument to be compensated for such that all instruments perform identically. The block heat sink assembly can be changed to another of the same or different design. The assembly carries the necessary information required to characterize its own performance in an on-board memory device, allowing the assembly to be interchangeable among instruments while retaining its precision operating characteristics. The instrument monitors the thermoelectric devices and warns of changes in resistance that may result in failure.
摘要翻译: 使用组件,加热盖和内部计算机执行高精度PCR的方法。 该组件由样品块,多个珀耳帖热电装置和散热器组成,夹在一起。 样品块温度由计算机控制的热电装置专门改变。 控制软件包括校准诊断,其允许从仪器到仪器的热电冷却器的性能的变化被补偿,使得所有仪器执行相同的操作。 块式散热器组件可以改变为相同或不同设计的另一种。 该组件携带在板载存储器件中表征其自身性能所需的必要信息,允许组件在仪器之间可互换,同时保持其精密操作特性。 仪器监测热电器件,并警告可能导致故障的电阻变化。
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公开(公告)号:US08685717B2
公开(公告)日:2014-04-01
申请号:US11654238
申请日:2007-01-17
申请人: John G. Atwood , Adrian Fawcett , Keith S. Ferrara , Paul M. Hetherington , Richard W. Noreiks , Douglas E. Olsen , John R. Widomski , Charles M. Wittmer
发明人: John G. Atwood , Adrian Fawcett , Keith S. Ferrara , Paul M. Hetherington , Richard W. Noreiks , Douglas E. Olsen , John R. Widomski , Charles M. Wittmer
IPC分类号: C12M1/34
CPC分类号: B01L7/52 , B01L3/50853 , B01L2300/0829 , B01L2300/1822 , B01L2300/1827 , C12Q1/686 , F25B21/04 , F25B2321/021 , G01R27/205 , G05D23/1919 , H01L35/30 , Y10T137/1516
摘要: An apparatus comprising an assembly and a cover. The assembly comprises a sample block configured to receive a microtiter tray having a plurality of vials, each vial comprising a cap having a surface and a perimeter. The cover comprises a platen, vertically and horizontally displaceable in relationship to the sample block, the platen having a plurality of recesses. Each recess corresponds to a respective vial and is shaped and arranged to clear the surface of its respective cap when the cover is displaced onto the sample block. The cover includes a skirt in contact with and surrounding a perimeter of the platen. The skirt is configured for mating with a perimeter of a microtiter tray when a microtiter tray is received in the sample block, and also configured such that the cover contacts a microtiter tray when a microtiter tray is received in the sample block.
摘要翻译: 一种包括组件和盖的装置。 组件包括被配置为接收具有多个小瓶的微量滴定盘的样品块,每个小瓶包括具有表面和周边的盖。 该盖包括一个与样品块相关的垂直和水平移位的压板,该压板具有多个凹槽。 每个凹槽对应于相应的小瓶,并且当盖子移动到样品块上时,其形状和布置成使其相应盖的表面清除。 所述盖包括与所述压板的周边接触并围绕所述压板的周边的裙部。 当将微量滴定盘收纳在样品块中时,裙部配置成与微量滴定盘的周边配合,并且还构造成使得当微量滴定盘被接收在样品块中时,盖接触微量滴定盘。
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公开(公告)号:US20100173400A1
公开(公告)日:2010-07-08
申请号:US12686100
申请日:2010-01-12
申请人: John G. Atwood , Adrian Fawcett , Keith S. Ferrara , Paul M. Hetherington , Richard W. Noreiks , Douglas E. Olsen , John R. Widomski , Charles M. Wittmer
发明人: John G. Atwood , Adrian Fawcett , Keith S. Ferrara , Paul M. Hetherington , Richard W. Noreiks , Douglas E. Olsen , John R. Widomski , Charles M. Wittmer
CPC分类号: B01L7/52 , B01L3/50853 , B01L2300/0829 , B01L2300/1822 , B01L2300/1827 , C12Q1/686 , F25B21/04 , F25B2321/021 , G01R27/205 , G05D23/1919 , H01L35/30 , Y10T137/1516
摘要: An instrument for performing highly accurate PCR employing an assembly, a heated cover, and an internal computer, is provided. The assembly is made up of a sample block, a number of Peltier thermal electric devices, and a heat sink, clamped together. A control algorithm manipulates the current supplied to thermoelectric coolers such that the dynamic thermal performance of a block can be controlled so that pre-defined thermal profiles of sample temperature can be executed. The sample temperature is calculated instead of measured using a design specific model and equations. The control software includes calibration diagnostics which permit variation in the performance of thermoelectric coolers from instrument to instrument to be compensated for such that all instruments perform identically. The block/heat sink assembly can be changed to another of the same or different design. The assembly carries the necessary information required to characterize its own performance in an on-board memory device, allowing the assembly to be interchangeable among instruments while retaining its precision operating characteristics.
摘要翻译: 提供了一种使用组件,加热盖和内部计算机执行高精度PCR的仪器。 组件由样品块,多个珀耳帖热电器件和散热器组成,夹在一起。 控制算法控制提供给热电冷却器的电流,使得可以控制块的动态热性能,从而可以执行样本温度的预定义热分布。 计算样品温度,而不是使用设计特定的模型和方程来测量。 控制软件包括校准诊断,其允许从仪器到仪器的热电冷却器的性能的变化被补偿,使得所有仪器执行相同的操作。 块/散热器组件可以改变为相同或不同设计的另一个。 该组件携带在板载存储器件中表征其自身性能所需的必要信息,允许组件在仪器之间可互换,同时保持其精密操作特性。
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公开(公告)号:US07645070B2
公开(公告)日:2010-01-12
申请号:US11804298
申请日:2007-05-17
申请人: John G. Atwood , Adrian Fawcett , Keith S. Ferrara , Paul M. Hetherington , Richard W. Noreiks , Douglas E. Olsen , John R. Widomski , Charles M. Wittmer
发明人: John G. Atwood , Adrian Fawcett , Keith S. Ferrara , Paul M. Hetherington , Richard W. Noreiks , Douglas E. Olsen , John R. Widomski , Charles M. Wittmer
CPC分类号: B01L7/52 , B01L3/50853 , B01L2300/0829 , B01L2300/1822 , B01L2300/1827 , C12Q1/686 , F25B21/04 , F25B2321/021 , G01R27/205 , G05D23/1919 , H01L35/30 , Y10T137/1516
摘要: An instrument for performing highly accurate PCR employing an assembly, a heated cover, and an internal computer, is provided. The assembly is made up of a sample block, a number of Peltier thermal electric devices, and a heat sink, clamped together. A control algorithm manipulates the current supplied to thermoelectric coolers such that the dynamic thermal performance of a block can be controlled so that pre-defined thermal profiles of sample temperature can be executed. The sample temperature is calculated instead of measured using a design specific model and equations. The control software includes calibration diagnostics which permit variation in the performance of thermoelectric coolers from instrument to instrument to be compensated for such that all instruments perform identically. The block/heat sink assembly can be changed to another of the same or different design. The assembly carries the necessary information required to characterize its own performance in an on-board memory device, allowing the assembly to be interchangeable among instruments while retaining its precision operating characteristics.
摘要翻译: 提供了一种使用组件,加热盖和内部计算机执行高精度PCR的仪器。 组件由样品块,多个珀耳帖热电器件和散热器组成,夹在一起。 控制算法控制提供给热电冷却器的电流,使得可以控制块的动态热性能,从而可以执行样本温度的预定义热分布。 计算样品温度,而不是使用设计特定的模型和方程来测量。 控制软件包括校准诊断,其允许从仪器到仪器的热电冷却器的性能的变化被补偿,使得所有仪器执行相同的操作。 块/散热器组件可以改变为相同或不同设计的另一个。 该组件携带在板载存储器件中表征其自身性能所需的必要信息,允许组件在仪器之间可互换,同时保持其精密操作特性。
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公开(公告)号:US20080314431A1
公开(公告)日:2008-12-25
申请号:US12229404
申请日:2008-08-22
申请人: John G. Atwood , Adrian Fawcett , Keith S. Ferrara , Paul M. Hetherington , Richard W. Noreiks , Douglas E. Olsen , John R. Widomski , Charles M. Wittmer
发明人: John G. Atwood , Adrian Fawcett , Keith S. Ferrara , Paul M. Hetherington , Richard W. Noreiks , Douglas E. Olsen , John R. Widomski , Charles M. Wittmer
IPC分类号: H01L35/28
CPC分类号: B01L7/52 , B01L3/50853 , B01L2300/0829 , B01L2300/1822 , B01L2300/1827 , C12Q1/686 , F25B21/04 , F25B2321/021 , G01R27/205 , G05D23/1919 , H01L35/30 , Y10T137/1516
摘要: An instrument for performing highly accurate PCR employing an assembly, a heated cover, and an internal computer, is provided. The assembly is made up of a sample block, a number of Peltier thermal electric devices, and a heat sink, clamped together. A control algorithm manipulates the current supplied to thermoelectric coolers such that the dynamic thermal performance of a block can be controlled so that pre-defined thermal profiles of sample temperature can be executed. The sample temperature is calculated instead of measured using a design specific model and equations. The control software includes calibration diagnostics which permit variation in the performance of thermoelectric coolers from instrument to instrument to be compensated for such that all instruments perform identically. The block/heat sink assembly can be changed to another of the same or different design. The assembly carries the necessary information required to characterize its own performance in an on-board memory device, allowing the assembly to be interchangeable among instruments while retaining its precision operating characteristics.
摘要翻译: 提供了一种使用组件,加热盖和内部计算机执行高精度PCR的仪器。 组件由样品块,多个珀耳帖热电器件和散热器组成,夹在一起。 控制算法控制提供给热电冷却器的电流,使得可以控制块的动态热性能,从而可以执行样本温度的预定义热分布。 计算样品温度,而不是使用设计特定的模型和方程来测量。 控制软件包括校准诊断,其允许从仪器到仪器的热电冷却器的性能的变化被补偿,使得所有仪器执行相同的操作。 块/散热器组件可以改变为相同或不同设计的另一个。 该组件携带必要的信息,以在车载存储设备中表征自己的性能,允许组件在仪器之间可互换,同时保持其精确的操作特性。
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公开(公告)号:US20090213382A1
公开(公告)日:2009-08-27
申请号:US12330930
申请日:2008-12-09
申请人: David H. Tracy , Jennifer M. Brockman , Keith S. Ferrara , Martin Shenker , Robert Kersten , Steven E. Cohen , Gary Bodley , Patrick Tuxbury , Paul Hetherington , Enrico Picozza
发明人: David H. Tracy , Jennifer M. Brockman , Keith S. Ferrara , Martin Shenker , Robert Kersten , Steven E. Cohen , Gary Bodley , Patrick Tuxbury , Paul Hetherington , Enrico Picozza
IPC分类号: G01N21/55
CPC分类号: G01N21/553 , G01N21/253 , G01N21/7743 , Y10T137/86035
摘要: An array surface plasmon resonance (SPR) analysis instrument comprising a reflective SPR sensor array, a light source assembly arranged to project a collimated beam of light onto the reflective SPR sensor array to provide a reflected array image of the sensor array, and to scan the incident angle of the collimated beam of light over an angular range, and a detector assembly oriented to receive the reflected array image of the sensor array over the angular range, the detector assembly comprises a two-dimensional detector sensing element that is tilted with respect to the optical axis of the lens assembly in accordance with the Scheimpflug condition, and a lens assembly for focusing the reflected array image of said SPR sensor array onto said tilted detector sensing element. The lens assembly comprises an objective section which is arranged to produce a virtual image of the tilted reflected array at infinity, followed by an imaging section arranged to transform the virtual image of the tilted reflected array into a real tilted image on the tilted detector sensing element.
摘要翻译: 阵列表面等离子体共振(SPR)分析仪器,其包括反射SPR传感器阵列,光源组件,被布置成将准直光束投射到反射SPR传感器阵列上,以提供传感器阵列的反射阵列图像,并扫描 在角度范围内的准直光束的入射角以及定向成在角度范围内接收传感器阵列的反射阵列图像的检测器组件,检测器组件包括二维检测器感测元件,其相对于 根据Scheimpflug条件的透镜组件的光轴,以及用于将所述SPR传感器阵列的反射阵列图像聚焦到所述倾斜检测器感测元件上的透镜组件。 透镜组件包括物镜部分,该物镜部分布置成产生无限远的倾斜反射阵列的虚像,随后是成像部分,被配置为将倾斜的反射阵列的虚像转换成倾斜检测器感测元件上的实际倾斜图像 。
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