公开(公告)号：US20180251835A1

公开(公告)日：2018-09-06

申请号：US15905497

申请日：2018-02-26

申请人： Kaneka Corporation

发明人： Takashi Nishizono ,  Sotaro Sano ,  Shigehiko Miyamoto ,  Hozumi Tanaka

IPC分类号： C12Q1/6876

CPC分类号： C12Q1/6876 ,  C12M1/34 ,  C12N15/09 ,  C12Q1/68 ,  C12Q1/6834 ,  G01N33/53 ,  G01N33/543 ,  C12Q2531/113 ,  C12Q2545/101 ,  C12Q2565/625

摘要： A nucleic acid detection device includes at least one target detection portion and a control detection portion, wherein a first probe that captures a target nucleic acid is immobilized on the target detection portion, and wherein a second probe that captures a control nucleic acid and a third probe that captures the target nucleic acid are immobilized on the control detection portion.

公开(公告)号：US20180201922A1

公开(公告)日：2018-07-19

申请号：US15917010

申请日：2018-03-09

申请人： Kaneka Corporation

发明人： Motonori Okajima ,  Takashi Nishizono ,  Shigehiko Miyamoto ,  Hozumi Tanaka

IPC分类号： C12N15/10

CPC分类号： C12N15/1006 ,  C12M1/00 ,  C12M1/26 ,  C12N15/00 ,  C12Q1/24 ,  G01N2333/35

摘要： A method for separating a nucleic acid from a specimen includes accommodating a specimen in an accommodation space provided in a nucleic acid-collecting member, transferring a treatment reagent to the nucleic acid-collecting member by connecting a treatment reagent-accommodating container to a treatment reagent-supplying opening of the nucleic acid-collecting member, obtaining a mixture by mixing the specimen and the treatment reagent in a mixing space formed by combining an inner container space of the treatment reagent-accommodating container and the accommodation space of the nucleic acid-collecting member, and pressure-feeding the mixture by reducing a volume of the inner container space of the treatment reagent-accommodating container and discharging the mixture from an mixture-discharging opening of the nucleic acid-collecting member via a carrier that adsorbs the nucleic acid released in the mixture while allowing the mixture to permeate therethrough.

公开(公告)号：US11274339B2

公开(公告)日：2022-03-15

申请号：US16238309

申请日：2019-01-02

申请人： KANEKA CORPORATION

发明人： Shigehiko Miyamoto ,  Takashi Nishizono

IPC分类号： C12Q1/686 ,  C12N15/09 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6853 ,  C12Q1/6876 ,  C12Q1/68

摘要： A primer set includes a terminal primer A including, in its 3′-terminal part, a nucleotide sequence that hybridizes to a 3′-terminal part of a complementary sequence of a first target nucleic acid sequence, a k-th double-headed primer including two polynucleotides linked at their 5′ terminal sides, wherein one of the two polynucleotides includes, in its 3′-terminal part, a nucleotide sequence that hybridizes to a 3′-terminal part of a nucleotide sequence of a k-th target nucleic acid, and the other polynucleotide includes, in its 3′-terminal part, a nucleotide sequence that hybridizes to a 3′-terminal part of a complementary sequence of a (k+1)th target nucleic acid, and a terminal primer B including, in its 3′-terminal part, a nucleotide sequence that hybridizes to a 3′-terminal part of a nucleotide sequence of a N-th target nucleic acid.

公开(公告)号：US10934541B2

公开(公告)日：2021-03-02

申请号：US15917010

申请日：2018-03-09

申请人： Kaneka Corporation

发明人： Motonori Okajima ,  Takashi Nishizono ,  Shigehiko Miyamoto ,  Hozumi Tanaka

IPC分类号： C12N15/10 ,  C12M1/26 ,  C12M1/00 ,  C12N15/00 ,  C12Q1/24

摘要： A method for separating a nucleic acid from a specimen includes accommodating a specimen in an accommodation space provided in a nucleic acid-collecting member, transferring a treatment reagent to the nucleic acid-collecting member by connecting a treatment reagent-accommodating container to a treatment reagent-supplying opening of the nucleic acid-collecting member, obtaining a mixture by mixing the specimen and the treatment reagent in a mixing space formed by combining an inner container space of the treatment reagent-accommodating container and the accommodation space of the nucleic acid-collecting member, and pressure-feeding the mixture by reducing a volume of the inner container space of the treatment reagent-accommodating container and discharging the mixture from an mixture-discharging opening of the nucleic acid-collecting member via a carrier that adsorbs the nucleic acid released in the mixture while allowing the mixture to permeate therethrough.

公开(公告)号：US20190119727A1

公开(公告)日：2019-04-25

申请号：US16238309

申请日：2019-01-02

申请人： KANEKA CORPORATION

发明人： Shigehiko Miyamoto ,  Takashi Nishizono

IPC分类号： C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6853 ,  C12Q1/6876

摘要： A primer set includes a terminal primer A including, in its 3′-terminal part, a nucleotide sequence that hybridizes to a 3′-terminal part of a complementary sequence of a first target nucleic acid sequence, a k-th double-headed primer including two polynucleotides linked at their 5′ terminal sides, wherein one of the two polynucleotides includes, in its 3′-terminal part, a nucleotide sequence that hybridizes to a 3′-terminal part of a nucleotide sequence of a k-th target nucleic acid, and the other polynucleotide includes, in its 3′-terminal part, a nucleotide sequence that hybridizes to a 3′-terminal part of a complementary sequence of a (k+1)th target nucleic acid, and a terminal primer B including, in its 3′-terminal part, a nucleotide sequence that hybridizes to a 3′-terminal part of a nucleotide sequence of a N-th target nucleic acid.