公开(公告)号：US20130273544A1

公开(公告)日：2013-10-17

申请号：US13765677

申请日：2013-02-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Alexander VLASSOV ,  Mu Li ,  Emily Zeringer ,  Richard Conrad

IPC: G01N1/34

CPC classification number: G01N1/34 ,  C12Q1/6806 ,  G01N33/5005 ,  G01N33/5076 ,  G01N33/92

Abstract: Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.

公开(公告)号：US20210190809A1

公开(公告)日：2021-06-24

申请号：US17122324

申请日：2020-12-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Alexander VLASSOV ,  Timothy BARTA ,  Justin INSLEE ,  Ethan GENTERT ,  Robert SMITH ,  Sara THIELE ,  Carolyn ADAMS ,  Kraig KRUGER ,  Robert Schneider ,  Wade LUKIANOW ,  Jay GRECO ,  Jason JONES ,  David CERRONE ,  Sumonto GHOSH ,  Brian VAJDA ,  Andrew BASTA ,  Allan EVANS ,  Christopher CROWLEY ,  Robert SETTERQUIST ,  Anne Cox ,  Mark MAYERNICK

IPC: G01N35/10 ,  G01N1/40 ,  G01N21/59 ,  G01N35/00 ,  B01D61/58 ,  B01D69/02

Abstract: Purifying target biomolecules, such as nucleic acids or proteins, from a biological source is a time intensive process and is typically performed by a skilled technician or scientist owing to the highly technical nature of the work. Systems, devices, and methods disclosed herein enable the automated bioprocessing and purification of target biomolecules from a biological source. For example, an instrument and disposable cartridge are provided for automatedly isolating and purifying nucleic acids (such as plasmid DNA from a bacterial culture) or for isolating protein from any biological sample. Such an exemplary instrument and cartridge can work in concert to timely release, mix, and move the target biomolecule and various reagents and buffers through a target biomolecule purification process, resulting in a purified target biomolecule with less manual oversight than traditional approaches.

公开(公告)号：US20140178888A1

公开(公告)日：2014-06-26

申请号：US14191109

申请日：2014-02-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Alexander VLASSOV ,  Mu LI ,  Emily ZERINGER ,  Richard CONRAD

IPC: C12Q1/68

CPC classification number: G01N1/34 ,  C12Q1/6806 ,  G01N33/5005 ,  G01N33/5076 ,  G01N33/92

Abstract: Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.

Abstract translation: 公开了用于从生物流体和组织分离外来体的方法，组合物和试剂盒。 体积排除聚合物用于从生物样品中沉淀外来体，从而通过低速（台式）离心或过滤来进行外来体分离。 还描述了沉淀后进一步分离外来体。

公开(公告)号：US20160320273A1

公开(公告)日：2016-11-03

申请号：US15142143

申请日：2016-04-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Alexander VLASSOV ,  Mu LI ,  Emily ZERINGER ,  Richard CONRAD

IPC: G01N1/34 ,  G01N33/50 ,  C12Q1/68

CPC classification number: G01N1/34 ,  C12Q1/6806 ,  G01N33/5005 ,  G01N33/5076 ,  G01N33/92

Abstract: Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.

公开(公告)号：US20150104801A1

公开(公告)日：2015-04-16

申请号：US14523764

申请日：2014-10-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Alexander VLASSOV ,  Mu LI ,  Emily ZERINGER ,  Richard CONRAD

IPC: C12Q1/68 ,  G01N1/34

CPC classification number: G01N1/34 ,  C12Q1/6806 ,  G01N33/5005 ,  G01N33/5076 ,  G01N33/92

Abstract: Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.

Abstract translation: 公开了用于从生物流体和组织分离外来体的方法，组合物和试剂盒。 体积排除聚合物用于从生物样品中沉淀外来体，从而通过低速（台式）离心或过滤来进行外来体分离。 还描述了沉淀后进一步分离外来体。

公开(公告)号：US20180195057A1

公开(公告)日：2018-07-12

申请号：US15909789

申请日：2018-03-01

Applicant: Life Technologies Corporation

Inventor： Alexander VLASSOV ,  Sarah LAROCCA ,  Mu LI

IPC: C12N15/10 ,  C12Q1/6806 ,  G01N33/574 ,  G01N33/569

Abstract: Compositions and methods for nucleic acid isolation from an environmental or biological sample comprising nucleic acid-analysis interferents, particularly from microbiome-containing samples, are provided.