公开(公告)号：US09476093B2

公开(公告)日：2016-10-25

申请号：US14593858

申请日：2015-01-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Lei Xi ,  Paul Kenney ,  Zhaochun Ma ,  Dennis Prosen ,  Stephen Hendricks ,  Roland Nagel

IPC: C12Q1/68 ,  C12P19/34 ,  C07H21/00 ,  C12N15/10

CPC classification number: C12Q1/686 ,  C07H21/00 ,  C07H21/02 ,  C12N9/1252 ,  C12N15/10 ,  C12Q1/6869 ,  C12Y207/07007

Abstract: This disclosure relates to methods of performing activation by polyphosphorolysis (APP) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or μ-monothiopyrophosphate), and related compounds.

公开(公告)号：US20160032262A1

公开(公告)日：2016-02-04

申请号：US14882343

申请日：2015-10-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Lei (Larry) XI ,  Dennis Prosen ,  Alexander Novikov

IPC: C12N9/12 ,  C12P19/34 ,  C12N9/88 ,  C12N9/96

CPC classification number: C12N9/1252 ,  C12N9/88 ,  C12N9/96 ,  C12P19/34 ,  C12Y207/07007 ,  C12Y402/99

Abstract: A modified thermostable Pol B DNA polymerase, produced by a reaction, under essentially aqueous conditions, of a thermostable Pol B DNA polymerase and a modifier reagent of Formula I wherein the reaction results in a thermally reversible inactivation of the thermostable Pol B DNA polymerase activity and the 3′-5′ exonuclease activity, which polymerase is suitable for hot-start PCR. Also disclosed are the method for the modification, a polynucleic acid amplification method and PCR reaction mixture and kit comprising the modified thermostable Pol B DNA polymerase.

Abstract translation: 通过在基本水溶液条件下通过热稳定的Pol B DNA聚合酶和式I的改性剂通过反应产生的经修饰的热稳定的Pol B DNA聚合酶，其中所述反应导致热可逆的Pol B DNA聚合酶活性的热可逆失活， 3'-5'核酸外切酶活性，该聚合酶适用于​​热启动PCR。 还公开了修饰方法，多核酸扩增方法和PCR反应混合物和试剂盒，其包含修饰的热稳定性Pol B DNA聚合酶。

公开(公告)号：US20150191775A1

公开(公告)日：2015-07-09

申请号：US14593858

申请日：2015-01-09

Applicant: Life Technologies Corporation

Inventor： Lei XI ,  Paul Kenney ,  Zhaochun Ma ,  Dennis Prosen ,  Stephen Hendricks ,  Roland Nagel

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C07H21/00 ,  C07H21/02 ,  C12N9/1252 ,  C12N15/10 ,  C12Q1/6869 ,  C12Y207/07007

Abstract: This disclosure relates to methods of performing activation by polyphosphorolysis (APP) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or μ-monothiopyrophosphate), and related compounds.

Abstract translation: 本公开涉及使用多磷酸盐，多磷酸盐，亚氨基二磷酸盐，硫代磷酸盐（或μ-一硫代焦磷酸盐）和相关化合物中的至少一种进行多磷酸解（APP）反应进行活化的方法。