公开(公告)号：US20200074624A1

公开(公告)日：2020-03-05

申请号：US16557376

申请日：2019-08-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jeffrey MARKS ,  David WOO ,  Wallace GEORGE

IPC: G06T7/00 ,  H04N5/372 ,  H04N5/347 ,  G01N27/447

Abstract: A biological analysis system and an associated method are provided. The method is used for recovering off scale data in an image produced by a camera in a capillary electrophoresis instrument. The method comprises the steps of identifying bins of the image where electron counts exceed a maximum number of counts; setting an off-scale flag for the identified bins; and processing the image to obtain a recovered dye signal, based on the flag set for each bin, and using a dye matrix.

公开(公告)号：US20160275687A1

公开(公告)日：2016-09-22

申请号：US14778415

申请日：2014-03-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yong CHU ,  Jeffrey MARKS

IPC: G06T7/00 ,  C12Q1/68 ,  G06T5/00

CPC classification number: G06T7/74 ,  C12Q1/686 ,  G06K9/00 ,  G06K2209/07 ,  G06T5/002 ,  G06T7/0012 ,  G06T7/11 ,  G06T7/136 ,  G06T7/194 ,  G06T2207/10064 ,  G06T2207/20024 ,  G06T2207/20208 ,  G06T2207/30072

Abstract: A method for analyzing biological reaction systems is provided. The method includes receiving an image of a substrate including a plurality of reaction sites after a biological reaction has taken place. Next, the method includes removing a noise background from the first image. The method includes determining an initial position of each reaction site based on an intensity threshold to generate a initial position set, then refining the initial position set of each reaction site based on an expected pattern of locations of the plurality of reaction sites to generate a first refined position set. The method further includes determining a presence or absence of a fluorescent emission from each reaction site based on the first refined position set and the first image.

Abstract translation: 提供了分析生物反应体系的方法。 该方法包括在发生生物反应之后接收包括多个反应位点的底物的图像。 接下来，该方法包括从第一图像中去除噪声背景。 该方法包括基于强度阈值确定每个反应位点的初始位置以产生初始位置集合，然后基于多个反应位点的预期位置模式来精化每个反应位点的初始位置集合，以产生第一 精致的位置集。 该方法还包括基于第一精制位置集和第一图像来确定来自每个反应位点的荧光发射的存在或不存在。

公开(公告)号：US20190126281A1

公开(公告)日：2019-05-02

申请号：US16116801

申请日：2018-08-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yong CHU ,  Jeffrey MARKS ,  Jacob FREUDENTHAL ,  Mingsong CHEN ,  Tiong Han TOH ,  Mauro AGUANNO ,  Lik Seng LAU ,  Lian Seng LOH ,  Kok Siong TEO ,  Zeng Wei CHU ,  Xin MATHERS ,  Michael UY ,  Huei Steven YEO ,  Kuan Moon BOO ,  Way Xuang LEE ,  Chin Yong KOO ,  Wei Fuh TEO ,  Soo Yong LAU ,  Hon Siu SHIN ,  Zeqi TAN ,  Thomas WESSEL ,  David WOO

IPC: B01L7/00 ,  G01N21/27 ,  G01N21/64 ,  C12Q1/6806 ,  G01N35/02

Abstract: A biological analysis system is provided. The system comprises a sample block assembly. The sample block assembly comprises a sample block configured to accommodate a sample holder, the sample holder configured to receive a plurality of samples. The system also comprises a control system configured to cycle the plurality of samples through a series of temperatures. The system further comprises an automated tray comprising a slide assembly, the tray configured to reversibly slide the sample block assembly from a closed to an open position to allow user access to the plurality of sample holders.