公开(公告)号：US20230296513A1

公开(公告)日：2023-09-21

申请号：US18006551

申请日：2021-07-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jacob FREUDENTHAL ,  Scott BENSON

IPC: G01N21/64 ,  G01N21/27 ,  C12Q1/6851 ,  C12Q1/686

CPC classification number: G01N21/6428 ,  G01N21/645 ,  G01N21/274 ,  C12Q1/6851 ,  C12Q1/686 ,  G01N2021/6421 ,  G01N2021/6439

Abstract: Energy transfer dye pairs including a donor dye covalently attached to an acceptor dye through a linker, uses of the energy transfer dye pairs, for example, in conjugates of an energy transfer dye pair covalently attached to a quencher and an analyte (e.g., an oligonucleotide), for biological applications including, for example, amplification assays such as quantitative polymerase chain reaction (qPCR) and digital polymerase chain reaction (dPCR). Systems and methods include those in which (1) two dyes have the same excitation wavelength range, but different emission wavelength ranges and/or (2) two dyes have the same emission wavelength range, but different excitation wavelength ranges.

公开(公告)号：US20230304932A1

公开(公告)日：2023-09-28

申请号：US18006559

申请日：2021-07-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Scott BENSON ,  Steven MENCHEN ,  Chu-An CHANG ,  Linda LEE ,  Jacob FREUDENTHAL ,  Khairuzzaman MULLAH

IPC: G01N21/64

CPC classification number: G01N21/6428 ,  G01N21/6452 ,  G01N21/6486 ,  G01N2021/6419 ,  G01N2021/6441

Abstract: A system (1000) comprising first and second excitation sources (101a, 101b) with respective excitation wavelengths for exciting first second and third dyes in a sample (110) and further comprising a detector (115), first and second emission spectral elements (121a, 121b) for transmitting respective first and second emission wavelengths as well as a processor (130) for automatically operating the elements of the system. The first dye comprises a first absorption spectrum comprising a first maximum absorption wavelength and the second dye comprises a second absorption spectrum comprising a second maximum absorption wavelength that is equal to or substantially equal to the first maximum absorption wavelength. The second dye comprises a second emission spectrum comprising a second maximum emission wavelength and the third dye comprises a third emission spectrum comprising a third maximum emission wavelength that is equal to or substantially equal to the second maximum emission wavelength.

公开(公告)号：US20190126281A1

公开(公告)日：2019-05-02

申请号：US16116801

申请日：2018-08-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yong CHU ,  Jeffrey MARKS ,  Jacob FREUDENTHAL ,  Mingsong CHEN ,  Tiong Han TOH ,  Mauro AGUANNO ,  Lik Seng LAU ,  Lian Seng LOH ,  Kok Siong TEO ,  Zeng Wei CHU ,  Xin MATHERS ,  Michael UY ,  Huei Steven YEO ,  Kuan Moon BOO ,  Way Xuang LEE ,  Chin Yong KOO ,  Wei Fuh TEO ,  Soo Yong LAU ,  Hon Siu SHIN ,  Zeqi TAN ,  Thomas WESSEL ,  David WOO

IPC: B01L7/00 ,  G01N21/27 ,  G01N21/64 ,  C12Q1/6806 ,  G01N35/02

Abstract: A biological analysis system is provided. The system comprises a sample block assembly. The sample block assembly comprises a sample block configured to accommodate a sample holder, the sample holder configured to receive a plurality of samples. The system also comprises a control system configured to cycle the plurality of samples through a series of temperatures. The system further comprises an automated tray comprising a slide assembly, the tray configured to reversibly slide the sample block assembly from a closed to an open position to allow user access to the plurality of sample holders.

公开(公告)号：US20160168626A1

公开(公告)日：2016-06-16

申请号：US15048324

申请日：2016-02-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ronald DANEHY ,  Gary LIM ,  Jacob FREUDENTHAL

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  B01L3/50851 ,  B01L3/50855 ,  B01L3/50857 ,  B01L2200/026 ,  B01L2200/0642 ,  B01L2200/0684 ,  B01L2200/142 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0858 ,  B01L2300/0893 ,  B01L2300/161 ,  C12M23/12 ,  C12M23/38

Abstract: A case for containing a plurality of biological samples contains a base, a substrate and a cover. The substrate is attached to the base and comprises a plurality of reaction regions configured to receive one or more biological samples. The cover comprises an inner surface and is configured to be attached to the base such that the base and cover together provide a cavity containing the substrate. The inner surface includes a central area and a recessed area disposed about the central area. The central area is configured to cover the reaction regions, while the recessed area is offset from the central area in a direction normal to the inner surface.

Abstract translation: 用于容纳多个生物样品的壳体包含基底，基底和盖。 底物附着到基底上，并且包括被配置成接收一个或多个生物样品的多个反应区域。 盖子包括内表面，并且被构造成附接到基座，使得基座和盖子一起提供容纳基底的空腔。 内表面包括中心区域和围绕中心区域设置的凹陷区域。 中心区域构造成覆盖反应区域，而凹陷区域在垂直于内表面的方向上偏离中心区域。